Detailed information NTG0275117

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: C7R87_RS12890
• Genome accession: NZ_CP027123
• Coordinates: 2383133..2384251 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain AR_0056
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2378133..2389251

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C7R87_RS12880 (C7R87_2366)	-	2381249..2381941 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
C7R87_RS12885 (C7R87_2367)	pilT	2382068..2383105 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
C7R87_RS12890 (C7R87_2368)	pilU	2383133..2384251 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
C7R87_RS12895 (C7R87_2369)	fur	2384321..2384758 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
C7R87_RS12900 (C7R87_2370)	-	2384870..2385268 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
C7R87_RS12905 (C7R87_2371)	-	2385323..2385646 (-)	324	WP_000442587.1	RnfH family protein	-
C7R87_RS12910 (C7R87_2372)	-	2385646..2386713 (-)	1068	WP_002019572.1	hypothetical protein	-
C7R87_RS12920 (C7R87_2373)	-	2386866..2387321 (-)	456	WP_000782976.1	bacteriohemerythrin	-
C7R87_RS12925 (C7R87_2374)	-	2387574..2388422 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=275117 C7R87_RS12890 WP_000347039.1 2383133..2384251(+) (pilU) [Acinetobacter baumannii strain AR_0056]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=275117 C7R87_RS12890 WP_000347039.1 2383133..2384251(+) (pilU) [Acinetobacter baumannii strain AR_0056]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395