Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   C5I45_RS07250 Genome accession   NZ_CP027061
Coordinates   1383290..1383904 (+) Length   204 a.a.
NCBI ID   WP_015387983.1    Uniprot ID   A0A6A8LHQ3
Organism   Bacillus sp. ZY-1-1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1378290..1388904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5I45_RS07215 (C5I45_07205) aroE 1378977..1379819 (+) 843 WP_003152856.1 shikimate dehydrogenase -
  C5I45_RS07220 (C5I45_07210) yhbY 1379813..1380103 (+) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  C5I45_RS07225 (C5I45_07215) - 1380114..1380683 (+) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  C5I45_RS07230 (C5I45_07220) yqeK 1380673..1381254 (+) 582 WP_107994303.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  C5I45_RS07235 (C5I45_07225) rsfS 1381251..1381607 (+) 357 WP_003152864.1 ribosome silencing factor -
  C5I45_RS07240 (C5I45_07230) - 1381604..1382341 (+) 738 WP_107994304.1 class I SAM-dependent methyltransferase -
  C5I45_RS07245 (C5I45_07235) comER 1382410..1383231 (-) 822 WP_014305449.1 late competence protein ComER -
  C5I45_RS07250 (C5I45_07240) comEA 1383290..1383904 (+) 615 WP_015387983.1 helix-hairpin-helix domain-containing protein Machinery gene
  C5I45_RS07255 (C5I45_07245) - 1383971..1384540 (+) 570 WP_003152868.1 ComE operon protein 2 -
  C5I45_RS07260 (C5I45_07250) comEC 1384541..1386892 (+) 2352 WP_107994305.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  C5I45_RS07265 (C5I45_07255) - 1386911..1387045 (-) 135 WP_003152870.1 YqzM family protein -
  C5I45_RS07270 (C5I45_07260) holA 1387280..1388321 (+) 1042 Protein_1401 DNA polymerase III subunit delta -
  C5I45_RS07275 (C5I45_07265) rpsT 1388338..1388604 (-) 267 WP_003152876.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22255.41 Da        Isoelectric Point: 5.5211

>NTDB_id=274802 C5I45_RS07250 WP_015387983.1 1383290..1383904(+) (comEA) [Bacillus sp. ZY-1-1]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=274802 C5I45_RS07250 WP_015387983.1 1383290..1383904(+) (comEA) [Bacillus sp. ZY-1-1]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LHQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363

Multiple sequence alignment