Detailed information NTG0272172

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: C5B74_RS14385
• Genome accession: NZ_CP026750
• Coordinates: 2932026..2933144 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain WCHAB005133
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2927026..2938144

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C5B74_RS14375 (C5B74_14380)	-	2930142..2930834 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
C5B74_RS14380 (C5B74_14385)	pilT	2930961..2931998 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
C5B74_RS14385 (C5B74_14390)	pilU	2932026..2933144 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
C5B74_RS14390 (C5B74_14395)	fur	2933214..2933651 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
C5B74_RS14395 (C5B74_14400)	-	2933763..2934161 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
C5B74_RS14400 (C5B74_14405)	-	2934216..2934539 (-)	324	WP_000442587.1	RnfH family protein	-
C5B74_RS14405 (C5B74_14410)	-	2934539..2935606 (-)	1068	WP_002019572.1	hypothetical protein	-
C5B74_RS14415 (C5B74_14420)	-	2935759..2936214 (-)	456	WP_000782976.1	bacteriohemerythrin	-
C5B74_RS14420 (C5B74_14425)	-	2936467..2937315 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=272172 C5B74_RS14385 WP_000347039.1 2932026..2933144(+) (pilU) [Acinetobacter baumannii strain WCHAB005133]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=272172 C5B74_RS14385 WP_000347039.1 2932026..2933144(+) (pilU) [Acinetobacter baumannii strain WCHAB005133]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395