Detailed information NTG0270232

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BKM19_RS03570
• Genome accession: NZ_CP026558
• Coordinates: 589139..590203 (+)
• Length: 354 a.a.
• NCBI ID: WP_002551652.1
• Uniprot ID: A0A7Z6Y2Y4
• Organism: Pseudomonas amygdali pv. morsprunorum strain R15244
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 584139..595203

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BKM19_RS03550 (BKM19_003545)	-	584337..585605 (+)	1269	WP_002551650.1	malic enzyme-like NAD(P)-binding protein	-
BKM19_RS03565 (BKM19_003560)	-	586488..588929 (-)	2442	WP_375233967.1	penicillin-binding protein 1A	-
BKM19_RS03570 (BKM19_003565)	pilM	589139..590203 (+)	1065	WP_002551652.1	pilus assembly protein PilM	Machinery gene
BKM19_RS03575 (BKM19_003570)	-	590203..590802 (+)	600	WP_060402732.1	PilN domain-containing protein	-
BKM19_RS03580 (BKM19_003575)	pilO	590799..591422 (+)	624	WP_002551654.1	type 4a pilus biogenesis protein PilO	-
BKM19_RS03585 (BKM19_003580)	pilP	591419..591946 (+)	528	WP_002551655.1	type 4a pilus biogenesis lipoprotein PilP	-
BKM19_RS03590 (BKM19_003585)	pilQ	591994..594099 (+)	2106	WP_060402733.1	type IV pilus secretin PilQ	Machinery gene
BKM19_RS03595 (BKM19_003590)	aroK	594104..594622 (+)	519	WP_002551657.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38078.57 Da
• Isoelectric Point: 4.7008

>NTDB_id=270232 BKM19_RS03570 WP_002551652.1 589139..590203(+) (pilM) [Pseudomonas amygdali pv. morsprunorum strain R15244]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=270232 BKM19_RS03570 WP_002551652.1 589139..590203(+) (pilM) [Pseudomonas amygdali pv. morsprunorum strain R15244]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCGTTGCCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAGCTCGAAGGGGTTGGTCAGGCGCTGTCTCGCGTGCTTGCCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTC
GCGGTAGCCGGTTCCGCCGTGATCACCAAGACGATTGAAATGGACGCCGGTCTTTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAGGCTGACCAGTACATCCCTTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAATCCCGAACGCGTCGAAGTATTGCTGGCTGCCTGCCGCAAAGAGAATGTCGAAGTGCGTGAGGCGGCGCTGGCTCTG
GCCGGGCTGACCGCGCGCGTGGTGGACGTCGAGGCCTATGCCCTGGAGCGCTCGTTCGGCCTGCTGGCTGCGCAACTGGG
TAACGGTCATGACGAGCTTACCGTGGCGGTGGTCGATATCGGTGCCACAATGACCACCCTCAGCGTGCTGCATCATGGCC
GGATCATCTATACCCGCGAGCAACTGTTTGGTGGCCGCCAGCTGACTGACGAAATCCAGCGCCGCTATGGGCTTTCCATG
GAGGAGGCCGGGCTTGCCAAGAAGCAGGGCGGGTTGCCCGACGACTATGTCAGCGAGGTGCTGGACCCCTTCAAGGATGC
GTTGGTGCAGCAGGTCTCTCGTTCTTTGCAGTTCTTCTTTGCGGCCGGTCAATACAACTCCGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAACGACGCATTGGCACCCCGACGATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGCTCCAAGGTCAATGCCGGGGCGCTGGCCAGTGACGCTCCCGCGTTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A7Z6Y2Y4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469