Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   C2124_RS14615 Genome accession   NZ_CP026307
Coordinates   3122942..3124441 (+) Length   499 a.a.
NCBI ID   WP_174265467.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain IBSBF 2571     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3117942..3129441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2124_RS14590 (C2124_14595) - 3119121..3120104 (+) 984 WP_014618022.1 KpsF/GutQ family sugar-phosphate isomerase -
  C2124_RS14595 (C2124_14600) - 3120137..3120724 (+) 588 WP_014618023.1 KdsC family phosphatase -
  C2124_RS14600 (C2124_14605) lptC 3120726..3121358 (+) 633 WP_014618024.1 LPS export ABC transporter periplasmic protein LptC -
  C2124_RS14605 (C2124_14610) lptA 3121426..3122022 (+) 597 WP_014618025.1 lipopolysaccharide transport periplasmic protein LptA -
  C2124_RS14610 (C2124_14615) lptB 3122056..3122844 (+) 789 WP_014618026.1 LPS export ABC transporter ATP-binding protein -
  C2124_RS14615 (C2124_14620) rpoN/rpoN1 3122942..3124441 (+) 1500 WP_174265467.1 RNA polymerase factor sigma-54 Machinery gene
  C2124_RS14620 (C2124_14625) hpf 3124493..3124846 (+) 354 WP_003262275.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  C2124_RS14625 (C2124_14630) ptsN 3125196..3125651 (+) 456 WP_003262274.1 PTS IIA-like nitrogen regulatory protein PtsN -
  C2124_RS14630 (C2124_14635) hprK 3125722..3126696 (+) 975 WP_003262273.1 HPr(Ser) kinase/phosphatase -
  C2124_RS14635 (C2124_14640) - 3126887..3127219 (+) 333 WP_039550978.1 PsiF family protein -
  C2124_RS14640 (C2124_14645) rapZ 3127314..3128204 (+) 891 WP_014618030.1 RNase adapter RapZ -
  C2124_RS14645 (C2124_14650) - 3128282..3128935 (+) 654 WP_174265468.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55170.61 Da        Isoelectric Point: 4.8496

>NTDB_id=268059 C2124_RS14615 WP_174265467.1 3122942..3124441(+) (rpoN/rpoN1) [Ralstonia solanacearum strain IBSBF 2571]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVSALQSNSATPLPTE
SPPPAANGSDHADTAEAPYENGSDFDSDYGSDRSDWSLDDFARKPQSDEDDRAPMQLREAEPTLREFLMEQLAPLKLSMR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLESPAKPLAL
AIVNQHLELLAARDYTRLKKALSVDEGALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQDARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=268059 C2124_RS14615 WP_174265467.1 3122942..3124441(+) (rpoN/rpoN1) [Ralstonia solanacearum strain IBSBF 2571]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
RYTGTCGACCATCGAACTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTGGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGATGGYTCGGTCAGCGCRCTGCAGAGCAACAGCGCCACCCCGCTGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGCTCGGACCACGCCGACACGGCCGAGGCACCGTACGAAAACGGCAGCGACTTCGACAG
CGACTACGGCTCCGATCGCTCCGACTGGAGCCTCGACGACTTCGCACGCAAACCCCAGTCCGACGAAGACGATCGCGCTC
CGATGCAGTTGCGCGAGGCCGAGCCCACGCTGCGCGAATTCCTGATGGAACAGCTGGCGCCGCTCAAATTGTCCATGCGT
GACAAGGGCCTCGTCATCTTCCTGATCGAGTCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGACGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAACTCGACGAACTGCAAGCCGCGCTGCGCATGCTGCAGAGCTTCGACCCGGCCG
GCATCGGTGCACGCTCGGCTGCGGAATGCCTGACGCTGCAGTTGCAGCGCCTGGAGTCGCCAGCCAAGCCGCTCGCGCTG
GCCATCGTCAACCAGCATCTCGAGCTGCTCGCCGCGCGCGATTACACGCGCCTGAAGAAAGCGCTGTCCGTCGACGAAGG
CGCGCTGAAGGCCGCGCATGAACTGATCCGCTCGCTGGCGCCGTTCCCGGGCCACGCCTTCGGGCGCGCCGAGGCCGACT
TCGTCGTGCCCGATGTCGTGGTGCGTAAAACCGCCGCAGGCTGGATGGCGCAACTGAACCCGGACGTGATGCCGCGCCTG
CGCATCAACGACATGTACGCCCAGATCCTGCGCAGCAGCCGCGGCGAGTCGGGCGCCGCCAACCTGCAGCAGAAGCTGCA
AGACGCGCGCTGGCTGATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCGATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAAATCCCAGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

97.395

100

0.974

Multiple sequence alignment