Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   SmaCSM2_RS17290 Genome accession   NZ_CP025298
Coordinates   3762356..3763414 (-) Length   352 a.a.
NCBI ID   WP_014038321.1    Uniprot ID   A0AAP7J1E4
Organism   Stenotrophomonas maltophilia strain CSM2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3757356..3768414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SmaCSM2_RS17270 (SmaCSM2_17270) - 3758285..3760255 (-) 1971 WP_049442715.1 type IV pilus secretin PilQ family protein -
  SmaCSM2_RS17275 (SmaCSM2_17275) - 3760277..3760810 (-) 534 WP_005418914.1 pilus assembly protein PilP -
  SmaCSM2_RS17280 (SmaCSM2_17280) pilO 3760810..3761466 (-) 657 WP_005418916.1 type 4a pilus biogenesis protein PilO -
  SmaCSM2_RS17285 (SmaCSM2_17285) - 3761463..3762356 (-) 894 WP_101766009.1 PilN domain-containing protein -
  SmaCSM2_RS17290 (SmaCSM2_17290) pilM 3762356..3763414 (-) 1059 WP_014038321.1 pilus assembly protein PilM Machinery gene
  SmaCSM2_RS17295 (SmaCSM2_17295) - 3763634..3766057 (+) 2424 WP_101766010.1 penicillin-binding protein 1A -
  SmaCSM2_RS17300 (SmaCSM2_17300) - 3766313..3766957 (+) 645 WP_012511978.1 hypothetical protein -
  SmaCSM2_RS17305 (SmaCSM2_17305) - 3767302..3768045 (-) 744 WP_101766011.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37741.35 Da        Isoelectric Point: 4.4006

>NTDB_id=259896 SmaCSM2_RS17290 WP_014038321.1 3762356..3763414(-) (pilM) [Stenotrophomonas maltophilia strain CSM2]
MGLIPKSQSPLVGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAMNRSGSKAKLAAAA
VAGSAVITKVIPMPADLDENDMEAQIELEAVNYIPYPIEEVNLDFEVIGSIPNNPEMVQVLLAASRSENVELRQSALELG
GLQAKVMDVEAFAVENAYALVASELPVSIDGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEIMRRYGLSYEE
AGLAKRQGGLPESYEMEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAVLGGLPEMVEEQLGVPTVVANPLA
QMTLGPKVNAHALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=259896 SmaCSM2_RS17290 WP_014038321.1 3762356..3763414(-) (pilM) [Stenotrophomonas maltophilia strain CSM2]
GTGGGGCTCATCCCAAAAAGTCAGTCGCCGCTTGTAGGCGTCGACATCAGTTCGACTGCGGTAAAGCTTTTGCAGCTGTC
CCGCAGCGGCAATCGTTTCCGCGTGGAACATTACGCTGTGGAACCTCTTCCGCCGAATGCGGTGGTGGAGAAGAACATCG
TCGAAGTGGAGGCCGTCGGTGAGGCCATCCGCCGGGCGATGAACCGTTCAGGCAGCAAGGCCAAGTTGGCCGCTGCCGCC
GTCGCGGGGTCGGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGATCTCGACGAAAACGACATGGAAGCCCAGATCGA
GCTGGAAGCGGTCAACTACATTCCGTATCCGATCGAGGAAGTGAACCTGGACTTCGAGGTGATCGGGTCGATCCCGAACA
ACCCGGAGATGGTCCAGGTGCTGCTGGCCGCGTCGCGTTCGGAGAATGTCGAGCTTCGCCAGTCCGCACTGGAACTCGGT
GGCCTGCAGGCCAAGGTGATGGACGTGGAGGCCTTCGCGGTCGAGAACGCCTACGCTCTGGTCGCCAGCGAGCTGCCGGT
GTCCATCGACGGCGTGGTCGCGCTGGTCGACATCGGCGCCACCATGACCACCCTCAATGTCCTGCGCGGCGGCCGTAGCC
TCTACAGCCGTGAGCAGGTGTTCGGTGGCAAGCAGCTGACCGACGAGATCATGCGCCGCTACGGCCTGAGCTACGAGGAA
GCCGGGCTGGCCAAGCGCCAGGGCGGCCTGCCGGAAAGCTACGAGATGGAAGTGCTGGAGCCGTTCAAGGAAGCCACGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTATGCAGGCAGCGAATTCAACCGCGTCGATCACATCGTGCTGGCCGGCG
GTTGCGCTGTGCTGGGCGGCCTGCCGGAGATGGTCGAGGAGCAGCTGGGTGTGCCGACCGTGGTCGCCAACCCGCTGGCG
CAGATGACCCTGGGGCCGAAGGTGAACGCGCATGCGTTGGCCCAGGATGCCCCCGCGCTGATGATCGCGACCGGTCTGGC
GCTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.42

100

0.514
  comM Acinetobacter nosocomialis M2

51.42

100

0.514
  comM Acinetobacter baylyi ADP1

50.284

100

0.503
  pilM Legionella pneumophila strain ERS1305867

47.863

99.716

0.477

Multiple sequence alignment