Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CW740_RS10480 Genome accession   NZ_CP025120
Coordinates   2246655..2247722 (-) Length   355 a.a.
NCBI ID   WP_106647448.1    Uniprot ID   -
Organism   Kangiella profundi strain FT102     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2241655..2252722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CW740_RS10455 (CW740_10455) aroK 2242156..2242680 (-) 525 WP_106647443.1 shikimate kinase AroK -
  CW740_RS10460 (CW740_10460) - 2242777..2244882 (-) 2106 WP_106647444.1 type IV pilus secretin PilQ -
  CW740_RS10465 (CW740_10465) - 2244894..2245454 (-) 561 WP_227523848.1 pilus assembly protein PilP -
  CW740_RS10470 (CW740_10470) - 2245451..2246080 (-) 630 WP_106647446.1 type 4a pilus biogenesis protein PilO -
  CW740_RS10475 (CW740_10475) - 2246080..2246655 (-) 576 WP_106647447.1 PilN domain-containing protein -
  CW740_RS10480 (CW740_10480) pilM 2246655..2247722 (-) 1068 WP_106647448.1 pilus assembly protein PilM Machinery gene
  CW740_RS10485 (CW740_10485) - 2248112..2250607 (+) 2496 WP_227523849.1 penicillin-binding protein 1A -
  CW740_RS10490 (CW740_10490) rpmE 2250700..2250909 (-) 210 WP_018624021.1 50S ribosomal protein L31 -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 38659.01 Da        Isoelectric Point: 4.4952

>NTDB_id=258470 CW740_RS10480 WP_106647448.1 2246655..2247722(-) (pilM) [Kangiella profundi strain FT102]
MVLGLFKKKQVPVIGIDISSTAVKVLQLGKVGNRYRVDRYAVEPLPQGVVVERDIRDTEAVGNAIKKAVDKARTTTKYAA
VAVSGSAVITRSIQMEKGLTDQEMEDVIKVEADQYIPYPLEEVNLDFQVLGDSEVNDNQVDVLLAASRSVNVDSRVDALE
IAGLIPKVVDIEAYAIERAYQLTKKKTVEHIENSVVAIVDIGATMTSLNVLRNGQVIYNREQLFGGNQLTEEIQSRYGLS
YQEAGLAKKQGSLPDDYETEVLIPFAEIITQQVGRALQFFFSASEFNSVNEIILAGGCASIDGLDGMIQEHLGTPTRVAN
PFAEMSISSHVNTQALSADAPAMMISCGLALRSFD

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=258470 CW740_RS10480 WP_106647448.1 2246655..2247722(-) (pilM) [Kangiella profundi strain FT102]
ATGGTTCTGGGGTTATTCAAAAAGAAACAAGTACCAGTCATCGGTATCGATATCAGCTCGACAGCTGTTAAGGTACTCCA
GCTTGGCAAGGTGGGGAATCGTTACAGAGTTGATCGTTATGCGGTAGAACCACTACCACAAGGTGTGGTGGTTGAGCGCG
ATATTCGTGATACTGAAGCCGTCGGCAATGCAATCAAGAAAGCTGTCGATAAAGCTCGTACAACAACAAAATATGCAGCC
GTGGCCGTTTCTGGTTCCGCGGTTATCACTCGTTCTATTCAGATGGAAAAAGGACTGACCGACCAAGAGATGGAAGACGT
CATCAAGGTCGAAGCAGATCAATATATTCCTTATCCATTGGAAGAAGTTAATCTGGACTTCCAGGTTTTGGGTGATTCAG
AAGTTAATGATAATCAGGTTGACGTACTATTAGCAGCGTCTCGAAGCGTGAACGTAGATTCGCGTGTTGATGCACTGGAA
ATTGCGGGCCTGATTCCAAAAGTGGTGGATATTGAAGCTTACGCCATTGAGCGTGCTTACCAATTAACCAAGAAGAAAAC
TGTTGAACATATCGAAAATTCGGTAGTGGCCATTGTCGATATCGGTGCGACCATGACCAGTTTGAATGTTTTGCGTAATG
GACAGGTTATTTATAACCGCGAGCAGTTATTTGGTGGTAACCAGCTAACTGAAGAAATTCAAAGCCGTTACGGTTTGTCA
TATCAAGAAGCTGGCTTAGCGAAAAAGCAGGGTAGCTTGCCTGATGATTATGAAACAGAAGTTCTAATTCCATTCGCAGA
AATTATCACCCAGCAGGTTGGACGCGCGTTGCAGTTCTTCTTCTCTGCGAGCGAATTTAATTCTGTTAATGAAATCATTT
TGGCGGGTGGTTGTGCCTCAATTGATGGCCTTGATGGCATGATTCAGGAGCACCTGGGAACCCCTACACGTGTTGCTAAC
CCATTTGCTGAAATGTCGATTTCTTCTCATGTGAATACTCAGGCTTTGAGTGCGGACGCTCCAGCAATGATGATTAGCTG
TGGACTAGCACTAAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(15-354)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50

99.718

0.499
  comM Acinetobacter nosocomialis M2

49.718

99.718

0.496
  comM Acinetobacter baylyi ADP1

47.175

99.718

0.47
  pilM Legionella pneumophila strain ERS1305867

46.328

99.718

0.462

Multiple sequence alignment