Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   CWI26_RS02680 Genome accession   NZ_CP025043
Coordinates   530077..531369 (+) Length   430 a.a.
NCBI ID   WP_024377223.1    Uniprot ID   -
Organism   Streptococcus suis strain AH681     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 511866..531369 530077..531369 within 0


Gene organization within MGE regions


Location: 511866..531369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI26_RS02600 (CWI26_02600) - 511866..513194 (+) 1329 WP_100881184.1 ISLre2 family transposase -
  CWI26_RS02605 (CWI26_02605) gmk 513623..514243 (+) 621 WP_024377213.1 guanylate kinase -
  CWI26_RS02610 (CWI26_02610) rpoZ 514276..514590 (+) 315 WP_004195201.1 DNA-directed RNA polymerase subunit omega -
  CWI26_RS02615 (CWI26_02615) - 514674..517064 (+) 2391 WP_100881185.1 primosomal protein N' -
  CWI26_RS02620 (CWI26_02620) fmt 517195..518133 (+) 939 WP_029179613.1 methionyl-tRNA formyltransferase -
  CWI26_RS02625 (CWI26_02625) rsmB 518120..519433 (+) 1314 WP_100881186.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  CWI26_RS02630 (CWI26_02630) - 519471..520208 (+) 738 WP_004194145.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  CWI26_RS02635 (CWI26_02635) pknB 520208..521737 (+) 1530 Protein_464 Stk1 family PASTA domain-containing Ser/Thr kinase -
  CWI26_RS12445 - 521738..522202 (+) 465 Protein_465 PASTA domain-containing protein -
  CWI26_RS02640 (CWI26_02640) - 522536..523657 (+) 1122 WP_100881187.1 IS110 family transposase -
  CWI26_RS02645 (CWI26_02645) liaF 524202..524897 (+) 696 WP_024377219.1 cell wall-active antibiotics response protein LiaF -
  CWI26_RS02650 (CWI26_02650) - 524894..525910 (+) 1017 WP_024419556.1 sensor histidine kinase -
  CWI26_RS02655 (CWI26_02655) - 525882..526523 (+) 642 WP_002937025.1 response regulator transcription factor -
  CWI26_RS02660 (CWI26_02660) - 526573..527973 (+) 1401 WP_029173958.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  CWI26_RS02665 (CWI26_02665) - 527975..528346 (+) 372 WP_002937017.1 S1 RNA-binding domain-containing protein -
  CWI26_RS02670 (CWI26_02670) cysK 528372..529298 (-) 927 WP_029185780.1 cysteine synthase A -
  CWI26_RS02675 (CWI26_02675) - 529388..530020 (-) 633 WP_024419558.1 YigZ family protein -
  CWI26_RS02680 (CWI26_02680) comFA/cflA 530077..531369 (+) 1293 WP_024377223.1 DEAD/DEAH box helicase Machinery gene

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 48994.55 Da        Isoelectric Point: 9.1584

>NTDB_id=257602 CWI26_RS02680 WP_024377223.1 530077..531369(+) (comFA/cflA) [Streptococcus suis strain AH681]
MKELENYYGRLFTKYQLTAKEREKAEKVPSITKKNNCFRCGTTFEEENKLPNDAYYCRACLLLGRVRSDEKLYHFPQKDF
SITKCLKWKGQLTDWQQRISDGLVANVENNRATLVHAVTGAGKTEMIYHTVASVIDKGGAVCLASPRIDVCIELYKRLQN
DFSVPISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLVLIDEVDAFPYADNPMLYRAADNAVKEDGVQVFLTATSTDEL
DKKVRMGKLSRLSLPRRFHGNPLVVPQKVWFSKFDDTLKKNRLVPKLKKAIEEQRKSGFPLLIFVPEISKGQEFTKIMKK
TFPEETIGFVSSQTENRLEIVEGFRKREITVLISTTILERGVTFPCVDVFVVQANHYLYTASSLVQIAGRVGRSMERPTG
LLQFYHEGSTGAIEKAIAEIKQMNKEAGYV

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=257602 CWI26_RS02680 WP_024377223.1 530077..531369(+) (comFA/cflA) [Streptococcus suis strain AH681]
ATGAAAGAATTAGAAAATTATTATGGAAGATTATTTACCAAATACCAATTGACAGCAAAAGAAAGAGAAAAAGCAGAAAA
AGTGCCAAGTATTACAAAAAAGAATAACTGCTTTCGCTGTGGAACAACTTTTGAAGAAGAAAACAAATTGCCAAACGATG
CTTATTACTGTCGAGCCTGCTTGCTTCTAGGCAGAGTACGGTCAGACGAAAAACTCTATCATTTTCCTCAGAAAGATTTT
TCAATCACTAAGTGTTTAAAGTGGAAAGGTCAATTAACTGATTGGCAACAAAGAATTTCAGATGGACTAGTTGCAAACGT
GGAAAATAATCGTGCGACATTGGTTCATGCAGTAACAGGAGCAGGTAAGACAGAAATGATCTACCACACCGTTGCCTCAG
TGATTGATAAAGGCGGAGCGGTTTGCCTAGCCAGTCCTCGAATTGATGTTTGTATCGAACTCTATAAACGTCTGCAAAAT
GACTTTTCAGTTCCAATTAGTTTACTGCATGGAGAGTCTGAACCCTATTTCCGAACTCCATTAGTTGTAGCAACCACACA
TCAGTTATTAAAATTTTATCAGGCCTTTGATTTGGTTTTGATTGATGAAGTAGACGCCTTTCCCTATGCAGATAATCCTA
TGCTCTATCGAGCAGCAGATAATGCGGTCAAGGAAGACGGAGTTCAAGTTTTTCTGACGGCGACTTCAACAGATGAATTG
GATAAAAAAGTCAGAATGGGTAAATTAAGCCGTCTTAGTTTGCCAAGGCGCTTTCATGGCAACCCACTTGTTGTCCCGCA
AAAAGTCTGGTTTAGTAAATTCGATGATACCCTAAAGAAAAATAGACTAGTTCCAAAGTTGAAAAAAGCGATTGAAGAAC
AGAGAAAGTCGGGCTTCCCCTTACTCATTTTTGTCCCAGAAATCTCCAAAGGTCAAGAATTTACCAAGATAATGAAAAAA
ACATTCCCAGAAGAAACAATTGGCTTTGTATCTAGTCAAACAGAAAATCGCCTTGAAATAGTTGAAGGGTTTCGCAAGAG
AGAAATCACAGTCTTAATCTCGACTACTATTCTTGAACGTGGGGTGACCTTCCCATGTGTAGACGTCTTTGTTGTTCAAG
CCAATCATTACCTCTACACAGCGTCAAGTCTCGTTCAGATTGCAGGTCGGGTTGGTAGGAGTATGGAACGTCCGACTGGT
TTACTCCAGTTTTATCATGAGGGAAGTACAGGAGCCATTGAAAAGGCAATCGCTGAAATTAAACAGATGAACAAGGAGGC
TGGTTATGTCTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(91-235)

Helicase conserved C-terminal domain (Helicase_C)

(289-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

67.053

100

0.672
  comFA/cflA Streptococcus pneumoniae Rx1

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae D39

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae R6

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae TIGR4

66.357

100

0.665
  comFA/cflA Streptococcus mitis SK321

66.125

100

0.663
  comFA Lactococcus lactis subsp. cremoris KW2

51.537

98.372

0.507
  comFA Latilactobacillus sakei subsp. sakei 23K

38.051

100

0.381
  comFA Bacillus subtilis subsp. subtilis str. 168

37.831

96.512

0.365

Multiple sequence alignment