Detailed information NTG0257531

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: DLM82_RS01145
• Genome accession: NZ_CP029749
• Coordinates: 190177..190638 (+)
• Length: 153 a.a.
• NCBI ID: WP_001878820.1
• Uniprot ID: -
• Organism: Streptococcus agalactiae strain PLGBS13
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 185177..195638

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DLM82_RS01110 (DLM82_01110)	-	186792..187157 (+)	366	WP_000285373.1	DUF1033 family protein	-
DLM82_RS01120 (DLM82_01120)	comGA	187330..188301 (+)	972	WP_000250805.1	competence type IV pilus ATPase ComGA	Machinery gene
DLM82_RS01125 (DLM82_01125)	comGB	188195..189238 (+)	1044	WP_223295889.1	competence type IV pilus assembly protein ComGB	Machinery gene
DLM82_RS01130 (DLM82_01130)	comGC/cglC	189235..189564 (+)	330	WP_000793380.1	competence type IV pilus major pilin ComGC	Machinery gene
DLM82_RS01135 (DLM82_01135)	comGD	189539..189952 (+)	414	WP_000244261.1	competence type IV pilus minor pilin ComGD	Machinery gene
DLM82_RS01140 (DLM82_01140)	comGE	189924..190223 (+)	300	WP_000234804.1	competence type IV pilus minor pilin ComGE	Machinery gene
DLM82_RS01145 (DLM82_01145)	comGF	190177..190638 (+)	462	WP_001878820.1	competence type IV pilus minor pilin ComGF	Machinery gene
DLM82_RS01150 (DLM82_01150)	comGG	190616..190987 (+)	372	WP_000601104.1	competence type IV pilus minor pilin ComGG	Machinery gene
DLM82_RS01155 (DLM82_01155)	comGH	191102..192076 (+)	975	WP_001008574.1	class I SAM-dependent methyltransferase	Machinery gene
DLM82_RS01160 (DLM82_01160)	-	192108..193301 (+)	1194	WP_000047534.1	acetate kinase	-
DLM82_RS01165 (DLM82_01165)	-	193453..193659 (+)	207	WP_000798241.1	helix-turn-helix transcriptional regulator	-
DLM82_RS01170 (DLM82_01170)	-	193718..193855 (+)	138	WP_001865900.1	hypothetical protein	-
DLM82_RS01175 (DLM82_01175)	-	193896..194351 (+)	456	WP_000905673.1	hypothetical protein	-
DLM82_RS01180 (DLM82_01180)	-	194420..195085 (+)	666	WP_000008113.1	CPBP family intramembrane glutamic endopeptidase	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17874.95 Da
• Isoelectric Point: 10.0299

>NTDB_id=257531 DLM82_RS01145 WP_001878820.1 190177..190638(+) (comGF) [Streptococcus agalactiae strain PLGBS13]
MKIIRKFCTYQKLRVKAFTLLKCLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQ
NKLYLRKQDKIVTFGKSNKDDFRKTGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET

Nucleotide

• Length: 462 bp

>NTDB_id=257531 DLM82_RS01145 WP_001878820.1 190177..190638(+) (comGF) [Streptococcus agalactiae strain PLGBS13]
TTGAAGATAATAAGGAAATTTTGCACGTATCAAAAGTTAAGGGTTAAAGCATTTACGCTTTTAAAATGTTTGGTAGCATT
GGTTACAATCACAGGAGCTTTACTAGTTTATCAAGGACTGACAAAATTGTTGGCTCAACAGATAGTAGTGATGTCTTCTT
CCAGTCAGTCTGAATGGGTGTTATTAACTCAGCAACTAAATGCAGAATTTGAAGGCGCTCATCTGGAATATTTAAGACAG
AACAAACTTTATTTACGTAAGCAAGATAAGATTGTAACCTTTGGCAAATCTAATAAAGATGATTTCCGTAAGACAGGTTA
TGATGGTCGAGGTTATCAACCAATGGTTTATGGGTTAGACAATTGTCAAATGAGTCAGACCAAAAGTATGGTAAAACTTG
TTTTTTATTTTAAGGACGGGTTAAAAAGGACATTTTACTATGATTTTAAAGAAGAAACTTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (49-142)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Streptococcus mutans UA159	47.183	92.81	0.438
comGF	Streptococcus mutans UA140	47.183	92.81	0.438
comGF/cglF	Streptococcus pneumoniae Rx1	42.446	90.85	0.386
comGF/cglF	Streptococcus pneumoniae D39	42.446	90.85	0.386
comGF/cglF	Streptococcus pneumoniae R6	42.446	90.85	0.386
comGF/cglF	Streptococcus pneumoniae TIGR4	42.446	90.85	0.386
comGF/cglF	Streptococcus mitis SK321	43.284	87.582	0.379
comGF/cglF	Streptococcus mitis NCTC 12261	42.537	87.582	0.373
comGF	Lactococcus lactis subsp. cremoris KW2	40	91.503	0.366