Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   CU118_RS05260 Genome accession   NZ_CP024998
Coordinates   1061368..1061865 (-) Length   165 a.a.
NCBI ID   WP_077446653.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 55-99-44     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1056368..1066865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CU118_RS05235 (CU118_05235) gcvPB 1056530..1058002 (-) 1473 WP_000202180.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  CU118_RS05240 (CU118_05240) gcvPA 1057995..1059341 (-) 1347 WP_054190021.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  CU118_RS05245 (CU118_05245) gcvT 1059361..1060452 (-) 1092 WP_054190022.1 glycine cleavage system aminomethyltransferase GcvT -
  CU118_RS05250 (CU118_05250) - 1060611..1061135 (-) 525 WP_054190023.1 shikimate kinase -
  CU118_RS05255 (CU118_05255) - 1061125..1061271 (-) 147 WP_001789879.1 hypothetical protein -
  CU118_RS05260 (CU118_05260) comGF 1061368..1061865 (-) 498 WP_077446653.1 competence type IV pilus minor pilin ComGF Machinery gene
  CU118_RS05265 (CU118_05265) comGE 1061783..1062082 (-) 300 WP_054190024.1 hypothetical protein Machinery gene
  CU118_RS05270 (CU118_05270) comGD 1062069..1062515 (-) 447 WP_077446655.1 competence type IV pilus minor pilin ComGD Machinery gene
  CU118_RS05275 (CU118_05275) comGC 1062493..1062804 (-) 312 WP_054190026.1 competence type IV pilus major pilin ComGC Machinery gene
  CU118_RS05280 (CU118_05280) comGB 1062818..1063888 (-) 1071 WP_054190027.1 competence type IV pilus assembly protein ComGB Machinery gene
  CU118_RS05285 (CU118_05285) comGA 1063860..1064834 (-) 975 WP_054191886.1 competence type IV pilus ATPase ComGA Machinery gene
  CU118_RS05290 (CU118_05290) - 1064886..1065509 (-) 624 WP_054190028.1 MBL fold metallo-hydrolase -
  CU118_RS05295 (CU118_05295) - 1065506..1065835 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  CU118_RS05300 (CU118_05300) - 1065835..1066821 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19129.87 Da        Isoelectric Point: 9.9584

>NTDB_id=257011 CU118_RS05260 WP_077446653.1 1061368..1061865(-) (comGF) [Staphylococcus aureus strain 55-99-44]
MILSKVTNKFVLLQKIPLLIKRHVYSINVKAFSLVEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRHQRIILHKGEEMIEYKLLNNKIIKVVNDEGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=257011 CU118_RS05260 WP_077446653.1 1061368..1061865(-) (comGF) [Staphylococcus aureus strain 55-99-44]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTACTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAGTTGAAATGTTAGTAGCGATGATGGTCATAAGTATAATTTTACTAATTGTTCCAGATT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCATCAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTATTGAATAATAAAATTATTAAAGTTGTAAATGACGAGGGTAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

95.758

100

0.958
  comGF Staphylococcus aureus MW2

94.545

100

0.945

Multiple sequence alignment