Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   CU118_RS05285 Genome accession   NZ_CP024998
Coordinates   1063860..1064834 (-) Length   324 a.a.
NCBI ID   WP_054191886.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 55-99-44     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1058860..1069834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CU118_RS05245 (CU118_05245) gcvT 1059361..1060452 (-) 1092 WP_054190022.1 glycine cleavage system aminomethyltransferase GcvT -
  CU118_RS05250 (CU118_05250) - 1060611..1061135 (-) 525 WP_054190023.1 shikimate kinase -
  CU118_RS05255 (CU118_05255) - 1061125..1061271 (-) 147 WP_001789879.1 hypothetical protein -
  CU118_RS05260 (CU118_05260) comGF 1061368..1061865 (-) 498 WP_077446653.1 competence type IV pilus minor pilin ComGF Machinery gene
  CU118_RS05265 (CU118_05265) comGE 1061783..1062082 (-) 300 WP_054190024.1 hypothetical protein Machinery gene
  CU118_RS05270 (CU118_05270) comGD 1062069..1062515 (-) 447 WP_077446655.1 competence type IV pilus minor pilin ComGD Machinery gene
  CU118_RS05275 (CU118_05275) comGC 1062493..1062804 (-) 312 WP_054190026.1 competence type IV pilus major pilin ComGC Machinery gene
  CU118_RS05280 (CU118_05280) comGB 1062818..1063888 (-) 1071 WP_054190027.1 competence type IV pilus assembly protein ComGB Machinery gene
  CU118_RS05285 (CU118_05285) comGA 1063860..1064834 (-) 975 WP_054191886.1 competence type IV pilus ATPase ComGA Machinery gene
  CU118_RS05290 (CU118_05290) - 1064886..1065509 (-) 624 WP_054190028.1 MBL fold metallo-hydrolase -
  CU118_RS05295 (CU118_05295) - 1065506..1065835 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  CU118_RS05300 (CU118_05300) - 1065835..1066821 (-) 987 WP_000161315.1 ROK family glucokinase -
  CU118_RS05305 (CU118_05305) - 1066818..1067021 (-) 204 WP_000087561.1 YqgQ family protein -
  CU118_RS05310 (CU118_05310) - 1067002..1068465 (-) 1464 WP_054190029.1 rhomboid family protein -
  CU118_RS05315 (CU118_05315) - 1068477..1069016 (-) 540 WP_077446656.1 5-formyltetrahydrofolate cyclo-ligase -
  CU118_RS05320 (CU118_05320) rpmG 1069225..1069374 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36912.97 Da        Isoelectric Point: 8.6529

>NTDB_id=257016 CU118_RS05285 WP_054191886.1 1063860..1064834(-) (comGA) [Staphylococcus aureus strain 55-99-44]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLIYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHDHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=257016 CU118_RS05285 WP_054191886.1 1063860..1064834(-) (comGA) [Staphylococcus aureus strain 55-99-44]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAATTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAAAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGTCCAACTGGTTCAGGTAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAGGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAGGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATGATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.383

100

0.994
  comGA Staphylococcus aureus N315

99.383

100

0.994

Multiple sequence alignment