Detailed information NTG0254262

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CTZ20_RS14775
• Genome accession: NZ_CP024611
• Coordinates: 3033700..3034818 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain Ab4977
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3028700..3039818

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CTZ20_RS14765 (CTZ20_14735)	-	3031816..3032508 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
CTZ20_RS14770 (CTZ20_14740)	pilT	3032635..3033672 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
CTZ20_RS14775 (CTZ20_14745)	pilU	3033700..3034818 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CTZ20_RS14780 (CTZ20_14750)	fur	3034888..3035325 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
CTZ20_RS14785 (CTZ20_14755)	-	3035437..3035835 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
CTZ20_RS14790 (CTZ20_14760)	-	3035890..3036213 (-)	324	WP_000442587.1	RnfH family protein	-
CTZ20_RS14795 (CTZ20_14765)	-	3036213..3037280 (-)	1068	WP_002019572.1	hypothetical protein	-
CTZ20_RS14805 (CTZ20_14775)	-	3037433..3037888 (-)	456	WP_000782976.1	bacteriohemerythrin	-
CTZ20_RS14810 (CTZ20_14780)	-	3038141..3038989 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=254262 CTZ20_RS14775 WP_000347039.1 3033700..3034818(+) (pilU) [Acinetobacter baumannii strain Ab4977]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=254262 CTZ20_RS14775 WP_000347039.1 3033700..3034818(+) (pilU) [Acinetobacter baumannii strain Ab4977]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395