Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   CS058_RS25635 Genome accession   NZ_CP024477
Coordinates   5356483..5357820 (+) Length   445 a.a.
NCBI ID   WP_031638292.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 12939     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5351483..5362820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CS058_RS25610 pgeF 5351626..5352354 (-) 729 WP_031756966.1 peptidoglycan editing factor PgeF -
  CS058_RS25615 rluD 5352351..5353313 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  CS058_RS25620 - 5353459..5354484 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  CS058_RS25625 - 5354641..5354886 (+) 246 WP_003102591.1 PP0621 family protein -
  CS058_RS25630 pilS 5354876..5356468 (+) 1593 WP_034024700.1 two-component system sensor histidine kinase PilS Regulator
  CS058_RS25635 pilR 5356483..5357820 (+) 1338 WP_031638292.1 two-component system response regulator PilR Regulator
  CS058_RS25640 thiO 5357866..5358960 (-) 1095 WP_023114840.1 glycine oxidase ThiO -
  CS058_RS25645 - 5359095..5359604 (+) 510 WP_034024702.1 GspH/FimT family pseudopilin -
  CS058_RS25650 - 5359710..5360216 (+) 507 WP_003110423.1 GspH/FimT family pseudopilin -
  CS058_RS25655 pilV 5360207..5360764 (+) 558 WP_003094697.1 type 4a pilus minor pilin PilV Machinery gene
  CS058_RS25660 pilW 5360761..5361585 (+) 825 WP_003119422.1 type 4a pilus minor pilin PilW -
  CS058_RS25665 pilX 5361582..5362169 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49737.06 Da        Isoelectric Point: 5.8638

>NTDB_id=253677 CS058_RS25635 WP_031638292.1 5356483..5357820(+) (pilR) [Pseudomonas aeruginosa strain 12939]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEIAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLADRI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=253677 CS058_RS25635 WP_031638292.1 5356483..5357820(+) (pilR) [Pseudomonas aeruginosa strain 12939]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGCAGCGGCAAGGAACTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAGCTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGTGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGATCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGATCGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTATACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCGGCGAGCCTGAGCGAAATCGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTATCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-115)

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

99.551

100

0.996
  pilR Acinetobacter baumannii strain A118

51.416

100

0.53
  luxO Vibrio cholerae strain A1552

38.17

100

0.384

Multiple sequence alignment