Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CRH16_RS09685 Genome accession   NZ_CP024412
Coordinates   1900888..1901340 (-) Length   150 a.a.
NCBI ID   WP_010787008.1    Uniprot ID   B8F829
Organism   Glaesserella parasuis strain SH0104     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1895888..1906340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRH16_RS09665 - 1896658..1897326 (-) 669 WP_015940025.1 prepilin peptidase -
  CRH16_RS09670 pilC 1897323..1898519 (-) 1197 WP_005711777.1 type II secretion system F family protein Machinery gene
  CRH16_RS09675 pilB 1898512..1899897 (-) 1386 WP_015940026.1 GspE/PulE family protein Machinery gene
  CRH16_RS09680 - 1900250..1900615 (-) 366 WP_015940027.1 endonuclease domain-containing protein -
  CRH16_RS09685 pilA 1900888..1901340 (-) 453 WP_010787008.1 pilin Machinery gene
  CRH16_RS09690 radA 1901528..1902904 (+) 1377 WP_021114521.1 DNA repair protein RadA -
  CRH16_RS09695 gmk 1902957..1903583 (-) 627 WP_021113158.1 guanylate kinase -
  CRH16_RS09700 gpt 1903709..1904188 (-) 480 WP_112063125.1 xanthine phosphoribosyltransferase -
  CRH16_RS09705 - 1904369..1905823 (+) 1455 WP_112063126.1 aminoacyl-histidine dipeptidase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 16138.74 Da        Isoelectric Point: 9.0811

>NTDB_id=253424 CRH16_RS09685 WP_010787008.1 1900888..1901340(-) (pilA) [Glaesserella parasuis strain SH0104]
MKKIRLIRPLAKAFTLIELMIVIAIIAILATIAIPSYTSYTQKAALSELLSASASYKSDVEICIYNVGDKKISECSSGKN
GVRADKSDVPDAKYLKSISVASGVITVAGKGNIDGYGYTMTPKFANNNITWATSCQGADISLFPENFCRQ

Nucleotide


Download         Length: 453 bp        

>NTDB_id=253424 CRH16_RS09685 WP_010787008.1 1900888..1901340(-) (pilA) [Glaesserella parasuis strain SH0104]
ATGAAAAAAATCAGGTTAATTCGACCGCTTGCAAAAGCCTTTACCTTAATTGAGTTAATGATCGTGATCGCCATCATTGC
CATTTTAGCAACAATAGCAATCCCGTCTTACACCAGTTACACCCAAAAGGCAGCTCTCTCTGAGTTATTAAGTGCTTCCG
CATCTTATAAATCCGATGTGGAAATTTGTATTTATAACGTAGGGGATAAAAAAATCTCGGAATGTAGTTCAGGTAAAAAT
GGCGTTCGAGCAGATAAATCCGATGTCCCTGACGCAAAATATCTTAAATCTATTTCCGTCGCTTCGGGGGTAATCACCGT
GGCAGGTAAAGGCAATATTGATGGCTACGGCTACACAATGACTCCGAAATTCGCCAATAACAACATCACTTGGGCAACAA
GCTGTCAAGGGGCTGACATTAGTTTGTTCCCTGAGAATTTCTGTAGGCAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B8F829

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

98.667

100

0.987
  pilA Haemophilus influenzae Rd KW20

54.054

98.667

0.533
  pilA Haemophilus influenzae 86-028NP

53.378

98.667

0.527

Multiple sequence alignment