Detailed information NTG0251153

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: BS636_RS12495
• Genome accession: NZ_CP024011
• Coordinates: 2569965..2571167 (+)
• Length: 400 a.a.
• NCBI ID: WP_099339062.1
• Uniprot ID: A0A2D1IRY4
• Organism: Acinetobacter sp. LoGeW2-3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2564965..2576167

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BS636_RS12480 (BS636_12475)	-	2566052..2566582 (+)	531	WP_099339059.1	glycine zipper domain-containing protein	-
BS636_RS12485 (BS636_12480)	-	2567104..2568153 (+)	1050	WP_099339060.1	3-deoxy-7-phosphoheptulonate synthase	-
BS636_RS12490 (BS636_12485)	pilU	2568397..2569575 (+)	1179	WP_099339061.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
BS636_RS12495 (BS636_12490)	pilU	2569965..2571167 (+)	1203	WP_099339062.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
BS636_RS12500 (BS636_12495)	-	2571230..2572408 (-)	1179	WP_099339063.1	MFS transporter	-
BS636_RS12505 (BS636_12500)	gcvH	2572639..2573013 (-)	375	WP_099339064.1	glycine cleavage system protein GcvH	-
BS636_RS12510 (BS636_12505)	-	2573086..2574114 (-)	1029	WP_099339065.1	AraC family transcriptional regulator	-
BS636_RS12515 (BS636_12510)	-	2574267..2574716 (-)	450	WP_099339066.1	MaoC family dehydratase	-

Sequence

Protein

• Length: 400 a.a.
• Molecular weight: 45058.39 Da
• Isoelectric Point: 6.3949

>NTDB_id=251153 BS636_RS12495 WP_099339062.1 2569965..2571167(+) (pilU) [Acinetobacter sp. LoGeW2-3]
MFTAELLEEARKFMHHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQELTPEKTKLFAYALMNEKQRKEYETELEC
NFAITVPGVSRFRVNVFQQQLNVGMVIRTITAEIPNFQKLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAALIDYRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDSHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
SNNANQTLDRIINFFPEERRNQLLMDLSSNMKAIVSQRLIRTEDGKGRRAAVEIMLNTPLVSDLILKGDFHELKAIMGKS
RELGMQTFDQALFDLYNEGAISYEEALRNADSTNELRLQIKLKSSRANAAANNTATAFSMVEEKKPDDETDEDKSTAEQA

Nucleotide

• Length: 1203 bp

>NTDB_id=251153 BS636_RS12495 WP_099339062.1 2569965..2571167(+) (pilU) [Acinetobacter sp. LoGeW2-3]
ATGTTCACGGCAGAGTTATTAGAAGAAGCACGTAAATTTATGCACCATATGTTGACCAAAGTGGTTGAATATGGTGGTTC
AGACTTGTTCATTACCGCGGACTTTCCACCTAGTATTAAACATCAGGGCTTAATGAAGCCTTTTGGGCAACAGGAACTTA
CCCCAGAGAAAACCAAACTGTTTGCTTATGCGTTAATGAATGAAAAGCAGCGTAAGGAATATGAAACCGAACTGGAATGT
AACTTTGCCATTACCGTACCGGGAGTTTCACGTTTCCGTGTCAACGTGTTCCAGCAGCAGCTGAATGTTGGCATGGTCAT
TCGTACCATTACCGCAGAAATTCCAAATTTCCAGAAACTAAAATTACCAGAATCGCTCAAGCATGTGATTATGGAAAAAC
GCGGTTTGGTACTGGTGGTAGGCGGTACAGGTTCAGGTAAGTCAACCTCACTGGCTGCGTTGATCGATTATCGTAATGAA
AACTCTGCTGGGCATATCATTACTGTAGAAGACCCGGTGGAATATGTACATAAACATAAGAAATCGATGATCACGCATCG
CGAAGTCGGTGTAGATAGCCATTCCTGGCACAATGCTTTAAAAAATACCTTGCGTCAGGCACCAGATGTAATTCTGATTG
GTGAGATTCGTGATACCGAAACCATGGAACATGCGATTGCTTTTGCTGAAACTGGGCATTTATGTTTGGGTACGCTGCAT
TCCAATAATGCCAACCAGACACTGGACCGCATTATTAACTTTTTCCCGGAAGAACGCCGTAACCAGTTGCTCATGGATCT
GTCTTCCAATATGAAAGCGATTGTCTCTCAACGATTGATCCGAACGGAAGATGGCAAGGGACGTCGTGCCGCAGTCGAGA
TCATGCTGAATACGCCGTTGGTATCTGACCTGATTTTGAAAGGCGATTTCCATGAGCTGAAAGCGATTATGGGCAAATCA
CGTGAACTCGGAATGCAGACTTTTGACCAGGCCTTATTTGATCTGTATAACGAAGGTGCGATTTCTTATGAAGAAGCATT
GCGTAATGCTGACTCGACCAATGAATTACGTCTGCAAATCAAACTGAAAAGTTCTCGTGCCAATGCAGCCGCGAATAATA
CAGCAACTGCATTTAGCATGGTTGAAGAGAAAAAGCCGGATGATGAAACTGACGAAGATAAAAGTACAGCAGAACAAGCT
TAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (117-282)

3D structure

• AlphaFold DB: A0A2D1IRY4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.632	87	0.527
pilU	Acinetobacter baylyi ADP1	54.19	89.5	0.485
pilU	Vibrio cholerae strain A1552	50.704	88.75	0.45