Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   CRH11_RS17450 Genome accession   NZ_CP023859
Coordinates   3510256..3511326 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain L-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3505256..3516326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRH11_RS17400 (CRH11_17395) sipW 3505316..3505900 (-) 585 WP_015240205.1 signal peptidase I SipW -
  CRH11_RS17405 (CRH11_17400) tapA 3505872..3506543 (-) 672 WP_020954230.1 amyloid fiber anchoring/assembly protein TapA -
  CRH11_RS17410 (CRH11_17405) - 3506802..3507131 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  CRH11_RS17415 (CRH11_17410) - 3507171..3507350 (-) 180 WP_003153093.1 YqzE family protein -
  CRH11_RS17420 (CRH11_17415) comGG 3507407..3507784 (-) 378 WP_012117980.1 competence type IV pilus minor pilin ComGG Machinery gene
  CRH11_RS17425 (CRH11_17420) comGF 3507785..3508285 (-) 501 WP_257899725.1 competence type IV pilus minor pilin ComGF -
  CRH11_RS17430 (CRH11_17425) comGE 3508194..3508508 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  CRH11_RS17435 (CRH11_17430) comGD 3508492..3508929 (-) 438 WP_043020787.1 competence type IV pilus minor pilin ComGD Machinery gene
  CRH11_RS17440 (CRH11_17435) comGC 3508919..3509227 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  CRH11_RS17445 (CRH11_17440) comGB 3509232..3510269 (-) 1038 WP_094031834.1 competence type IV pilus assembly protein ComGB Machinery gene
  CRH11_RS17450 (CRH11_17445) comGA 3510256..3511326 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  CRH11_RS17455 (CRH11_17450) - 3511520..3512470 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  CRH11_RS17460 (CRH11_17455) - 3512616..3513917 (+) 1302 WP_021494315.1 hemolysin family protein -
  CRH11_RS17465 (CRH11_17460) - 3513955..3514785 (-) 831 WP_007408317.1 STAS domain-containing protein -
  CRH11_RS17475 (CRH11_17470) - 3514992..3515372 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  CRH11_RS17480 (CRH11_17475) - 3515610..3515855 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=250583 CRH11_RS17450 WP_003153083.1 3510256..3511326(-) (comGA) [Bacillus velezensis strain L-1]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=250583 CRH11_RS17450 WP_003153083.1 3510256..3511326(-) (comGA) [Bacillus velezensis strain L-1]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTAAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment