Detailed information NTG0249176

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: MKLM6_RS20415
• Genome accession: NZ_CP023669
• Coordinates: 4543236..4544297 (-)
• Length: 353 a.a.
• NCBI ID: WP_064023024.1
• Uniprot ID: -
• Organism: Methylomonas koyamae strain LM6
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4538236..4549297

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MKLM6_RS24135	lptM	4538965..4539132 (-)	168	WP_197495914.1	LPS translocon maturation chaperone LptM	-
MKLM6_RS20395 (MKLM6_4003)	pilQ	4539243..4541492 (-)	2250	WP_064027554.1	type IV pilus secretin family protein	-
MKLM6_RS20400 (MKLM6_4004)	-	4541496..4542044 (-)	549	WP_064027552.1	pilus assembly protein PilP	-
MKLM6_RS20405 (MKLM6_4005)	-	4542044..4542667 (-)	624	WP_064023022.1	type 4a pilus biogenesis protein PilO	-
MKLM6_RS20410 (MKLM6_4006)	-	4542664..4543236 (-)	573	WP_054758945.1	PilN domain-containing protein	-
MKLM6_RS20415 (MKLM6_4007)	pilM	4543236..4544297 (-)	1062	WP_064023024.1	pilus assembly protein PilM	Machinery gene
MKLM6_RS20420 (MKLM6_4008)	-	4544603..4546855 (+)	2253	WP_232529276.1	penicillin-binding protein 1A	-
MKLM6_RS20425 (MKLM6_4009)	rpmE	4546916..4547116 (-)	201	WP_054758943.1	50S ribosomal protein L31	-
MKLM6_RS20430 (MKLM6_4010)	-	4547226..4548497 (-)	1272	WP_064027548.1	AmpG family muropeptide MFS transporter	-

Sequence

Protein

• Length: 353 a.a.
• Molecular weight: 37727.18 Da
• Isoelectric Point: 4.7083

>NTDB_id=249176 MKLM6_RS20415 WP_064023024.1 4543236..4544297(-) (pilM) [Methylomonas koyamae strain LM6]
MSWFSRNPSAVLGVDISTAAVKLLELSRAGARYKVESYAVVPLPQDAIVDKNITNVEQIGGAIKAAVKQSGTKAKHASVA
VAGSSVMTKIISMPASLSDAEMEEQIMVEADQYIPYSLDEVNLDFEVQGVTKSNPDMVDVLLAASRRENIEDRVAALAYA
GLKTVVVDVEAFAMENAFSLLVDQLPEGTGDMTVAIADIGATMTSLNILHAGKTVYTREQGFGGKQLTEEIQRRYGLSYE
EAGLAKKHGGLPDNYAADVLEPFKKAMLQQIARSLQFFVSSSANRGVDALILAGGCASIAGLDKLVERDLGIPSYIANPF
INMALSNRVKPQSLSNDTPAMMIACGLALRSFD

Nucleotide

• Length: 1062 bp

>NTDB_id=249176 MKLM6_RS20415 WP_064023024.1 4543236..4544297(-) (pilM) [Methylomonas koyamae strain LM6]
ATGAGCTGGTTCAGCAGAAATCCGTCGGCCGTGTTGGGTGTGGATATCAGTACTGCGGCGGTAAAGTTGTTGGAGCTAAG
TCGGGCCGGGGCGCGATACAAAGTCGAGAGTTACGCCGTAGTGCCGCTACCCCAGGATGCCATCGTCGACAAGAATATCA
CTAATGTCGAGCAAATTGGCGGAGCGATCAAGGCCGCAGTCAAACAGTCCGGCACTAAAGCCAAGCACGCGAGCGTCGCC
GTCGCCGGCTCTTCGGTGATGACGAAGATCATATCGATGCCGGCGTCGTTGTCCGATGCCGAGATGGAAGAACAGATTAT
GGTCGAAGCGGACCAATATATTCCGTATTCGCTGGACGAGGTAAATTTGGATTTCGAAGTCCAAGGCGTTACCAAAAGCA
ATCCGGACATGGTCGACGTATTGCTCGCCGCTTCCCGGCGGGAAAATATCGAAGACCGTGTTGCCGCGTTGGCCTATGCC
GGACTGAAGACCGTGGTGGTCGATGTCGAGGCATTCGCCATGGAAAACGCATTTTCGTTGCTGGTGGACCAGCTACCGGA
GGGGACCGGCGACATGACCGTGGCGATTGCCGATATCGGCGCCACGATGACCTCATTGAATATTTTGCATGCCGGAAAGA
CGGTTTACACTCGCGAGCAGGGGTTCGGCGGCAAACAATTAACCGAGGAAATCCAGCGCCGCTACGGACTTTCCTACGAA
GAGGCGGGATTAGCCAAGAAACATGGCGGCTTGCCCGACAACTATGCGGCCGATGTGTTGGAGCCGTTTAAAAAAGCCAT
GTTGCAGCAAATTGCCCGGTCATTGCAGTTTTTCGTTTCGTCCAGTGCCAACCGCGGCGTGGATGCGTTAATTCTCGCCG
GCGGCTGCGCATCGATAGCCGGTTTGGACAAATTGGTCGAACGCGATTTGGGCATACCGTCTTATATCGCCAATCCGTTT
ATCAATATGGCGCTGTCCAATCGGGTTAAGCCGCAAAGTCTCAGTAACGATACGCCGGCGATGATGATCGCTTGCGGCTT
GGCGTTAAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	51.977	100	0.521
comM	Acinetobacter nosocomialis M2	51.695	100	0.518
comM	Acinetobacter baylyi ADP1	49.575	100	0.496
pilM	Legionella pneumophila strain ERS1305867	42.151	97.45	0.411