Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   CP943_RS14305 Genome accession   NZ_CP023665
Coordinates   2809948..2810577 (-) Length   209 a.a.
NCBI ID   WP_026579269.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain Bac84     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2804948..2815577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CP943_RS14285 holA 2805507..2806532 (-) 1026 WP_188016690.1 DNA polymerase III subunit delta -
  CP943_RS14290 - 2806762..2806893 (+) 132 WP_003183686.1 YqzM family protein -
  CP943_RS14295 - 2806918..2809305 (-) 2388 WP_105981093.1 DNA internalization-related competence protein ComEC/Rec2 -
  CP943_RS14300 - 2809310..2809879 (-) 570 WP_026579270.1 ComE operon protein 2 -
  CP943_RS14305 comEA 2809948..2810577 (-) 630 WP_026579269.1 helix-hairpin-helix domain-containing protein Machinery gene
  CP943_RS14310 comER 2810660..2811481 (+) 822 WP_025811127.1 late competence protein ComER -
  CP943_RS14315 - 2811552..2812307 (-) 756 WP_105981094.1 class I SAM-dependent methyltransferase -
  CP943_RS14320 rsfS 2812304..2812660 (-) 357 WP_023855256.1 ribosome silencing factor -
  CP943_RS14325 yqeK 2812674..2813237 (-) 564 WP_026579268.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  CP943_RS14330 - 2813227..2813796 (-) 570 WP_025811125.1 nicotinate-nucleotide adenylyltransferase -
  CP943_RS14335 yhbY 2813815..2814105 (-) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  CP943_RS14340 aroE 2814099..2814935 (-) 837 WP_020452280.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22554.40 Da        Isoelectric Point: 4.7140

>NTDB_id=249047 CP943_RS14305 WP_026579269.1 2809948..2810577(-) (comEA) [Bacillus paralicheniformis strain Bac84]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSGFSIPEEASAQTFDKKEEVKREKNAGEEEVIIDLKGAVKNPGV
YQMKEGDRVHDALKKAGGTEKKADQKQINLAAVLRDGMVLYIPFEGEEAAGSLSEADSRADGSSGDIVNINTASSAELQT
IPGIGPSKAEAVIEYREENGMFQTIEDITNVSGIGEKSFERIKSSITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=249047 CP943_RS14305 WP_026579269.1 2809948..2810577(-) (comEA) [Bacillus paralicheniformis strain Bac84]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCGGCGGGAGGTGTGGCACTCGTCTTGATCATCAGCGCTGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCCGGCTTCTCCATTCCTGAAGAGGCTTCTGCACAGACTTTTGACAAGAAAG
AGGAAGTGAAGCGGGAAAAAAACGCGGGGGAAGAGGAGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAGGAGGGAGACAGGGTGCACGATGCATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGACCAAAAGCA
AATTAATCTGGCAGCCGTTTTGCGGGACGGTATGGTTCTTTACATTCCATTTGAAGGGGAAGAGGCCGCTGGCTCTCTTT
CGGAAGCGGACTCAAGAGCAGACGGCAGCTCAGGTGATATCGTCAATATCAATACGGCTTCCTCTGCGGAGCTTCAAACG
ATTCCCGGGATCGGTCCTTCAAAAGCGGAAGCGGTAATCGAATACCGCGAGGAGAACGGAATGTTTCAGACGATTGAAGA
CATAACAAACGTTTCGGGAATTGGAGAGAAGTCTTTTGAAAGAATAAAATCTTCCATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(146-207)

SLBB domain (SLBB)

(69-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

52.804

100

0.541
  comEA Staphylococcus aureus MW2

35.909

100

0.378
  comEA Staphylococcus aureus N315

35.455

100

0.373
  comEA Lactococcus lactis subsp. cremoris KW2

35.945

100

0.373

Multiple sequence alignment