Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   DBX32_RS27995 Genome accession   NZ_CP028848
Coordinates   5881648..5882271 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain IMP67     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5876648..5887271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBX32_RS27970 (DBX32_27895) - 5876979..5877272 (-) 294 Protein_5409 type IV pilus secretin PilQ -
  DBX32_RS27975 (DBX32_27900) - 5877371..5878717 (+) 1347 WP_079263478.1 ISNCY family transposase -
  DBX32_RS27980 (DBX32_27905) - 5878728..5879081 (-) 354 WP_079263477.1 SH3 domain-containing protein -
  DBX32_RS27985 (DBX32_27910) pilQ 5879205..5881073 (-) 1869 Protein_5412 type 4a pilus secretin PilQ -
  DBX32_RS27990 (DBX32_27915) comL/pilP 5881127..5881651 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  DBX32_RS27995 (DBX32_27920) pilO 5881648..5882271 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  DBX32_RS28000 (DBX32_27925) pilN 5882268..5882864 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  DBX32_RS28005 (DBX32_27930) pilM 5882864..5883928 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  DBX32_RS28010 (DBX32_27935) - 5884113..5886584 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=248712 DBX32_RS27995 WP_003103268.1 5881648..5882271(-) (pilO) [Pseudomonas aeruginosa strain IMP67]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=248712 DBX32_RS27995 WP_003103268.1 5881648..5882271(-) (pilO) [Pseudomonas aeruginosa strain IMP67]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401