Detailed information NTG0024817

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NOC_RS07385
• Genome accession: NC_007484
• Coordinates: 1507325..1508497 (-)
• Length: 390 a.a.
• NCBI ID: WP_002811372.1
• Uniprot ID: Q3JBE1
• Organism: Nitrosococcus oceani ATCC 19707
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1502325..1513497

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NOC_RS07365 (Noc_1357)	-	1503452..1504654 (+)	1203	WP_002808544.1	ChaN family lipoprotein	-
NOC_RS07370 (Noc_1358)	-	1504974..1505417 (+)	444	WP_002808880.1	YcgN family cysteine cluster protein	-
NOC_RS07375 (Noc_1359)	-	1505551..1506345 (-)	795	WP_002811172.1	hypothetical protein	-
NOC_RS07380 (Noc_1360)	-	1506444..1506905 (-)	462	WP_002808853.1	hypothetical protein	-
NOC_RS07385 (Noc_1361)	pilU	1507325..1508497 (-)	1173	WP_002811372.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NOC_RS07390 (Noc_1362)	-	1508623..1509540 (+)	918	WP_002810593.1	AEC family transporter	-
NOC_RS07395 (Noc_1363)	-	1509533..1510279 (-)	747	WP_002811494.1	competence/damage-inducible protein A	-
NOC_RS07400 (Noc_1364)	-	1510384..1512306 (+)	1923	WP_002810216.1	ATP-dependent DNA helicase	-
NOC_RS07405 (Noc_1365)	-	1512219..1513253 (-)	1035	WP_011330636.1	bile acid:sodium symporter family protein	-

Sequence

Protein

• Length: 390 a.a.
• Molecular weight: 43171.42 Da
• Isoelectric Point: 6.5079

>NTDB_id=24817 NOC_RS07385 WP_002811372.1 1507325..1508497(-) (pilU) [Nitrosococcus oceani ATCC 19707]
MEFKDYLKILVAHDGSDIYLSAGAPPCAKFHGLLKPLEKTRLSSSDIKKIAYDIMSEEQTAEFEQKPEMNLALSESGIGR
FRVNIFKQRSAISMVIRNIKTTIPNVDNLGLPPILKNVVMDKHGLILFVGATGSGKSTSLAALIDHRNNSSAGHIITIED
PIEYIHQHKRSIVNQREVGVDTESYGDALKNTLRQAPDVILIGEVRDRNTMEHAIAFAETGHLCLSTLHANNANQALDRI
INFFPEERRPQLLLDLSLNLRAFISQRLVPTIDGKRTAAIEILLGTPLVRDLIHRGEIHAIKDVMEKSENLGMQTFDSAL
YKLYATGLISVEEALRNADSPNNLRLKINLSKKAMGSTTASSATEKISLDEKPNKDELGGLSFSLVEEED

Nucleotide

• Length: 1173 bp

>NTDB_id=24817 NOC_RS07385 WP_002811372.1 1507325..1508497(-) (pilU) [Nitrosococcus oceani ATCC 19707]
ATGGAATTTAAGGATTACCTAAAAATACTTGTTGCCCACGATGGCTCAGATATTTATCTGAGCGCTGGAGCCCCCCCCTG
CGCCAAATTCCACGGTTTGCTGAAACCTCTCGAAAAAACGAGATTATCTTCCAGCGATATAAAGAAAATCGCCTACGACA
TCATGAGCGAAGAACAAACAGCCGAGTTTGAACAAAAACCGGAAATGAACTTGGCTCTTTCCGAATCCGGCATTGGACGC
TTTCGGGTCAATATTTTCAAACAGCGCAGTGCAATTTCCATGGTCATTAGGAATATCAAAACTACTATCCCTAACGTAGA
TAACCTCGGCCTGCCTCCTATATTAAAAAACGTTGTGATGGATAAGCATGGCCTCATTTTATTTGTAGGGGCAACCGGTT
CTGGTAAATCGACCTCTCTTGCCGCCCTCATTGATCATCGCAATAATAGCAGCGCGGGTCATATCATCACGATTGAAGAC
CCTATCGAGTATATACACCAGCATAAAAGATCCATCGTCAATCAGCGCGAAGTAGGCGTCGACACGGAGTCTTACGGAGA
TGCGCTTAAAAATACCCTACGCCAGGCACCGGATGTCATTCTTATTGGAGAAGTTCGCGACCGGAATACTATGGAACATG
CCATCGCTTTTGCGGAAACAGGTCACTTATGCCTTTCTACCCTTCATGCTAACAATGCTAACCAGGCGCTTGACCGAATT
ATCAACTTCTTCCCGGAAGAGCGGCGCCCACAGCTACTCTTGGATCTCTCTTTAAACCTGCGCGCTTTTATTTCCCAGCG
TCTGGTTCCCACCATTGACGGTAAGCGCACTGCGGCTATTGAGATTTTATTGGGGACGCCTCTGGTGCGCGACCTCATCC
ACCGGGGTGAAATCCACGCTATTAAAGACGTCATGGAAAAATCGGAGAATCTCGGTATGCAAACCTTCGATAGCGCCCTT
TATAAATTATATGCGACGGGCCTTATCAGTGTCGAAGAGGCGCTGCGCAACGCCGATTCCCCTAATAATCTCCGCCTCAA
AATTAACTTATCAAAAAAAGCAATGGGATCGACAACCGCTTCCTCCGCAACGGAAAAAATATCCCTGGATGAGAAACCTA
ACAAGGATGAATTGGGAGGGCTATCCTTCAGCCTGGTGGAGGAAGAAGACTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (44-270)

3D structure

• AlphaFold DB: Q3JBE1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.201	97.179	0.546
pilU	Acinetobacter baylyi ADP1	55.618	91.282	0.508
pilU	Vibrio cholerae strain A1552	50.135	95.128	0.477
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.12	94.359	0.397