Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PSHA_RS12810 Genome accession   NC_007481
Coordinates   2747002..2748138 (-) Length   378 a.a.
NCBI ID   WP_011329250.1    Uniprot ID   A0A1R4ISF5
Organism   Pseudoalteromonas translucida     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2742002..2753138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSHA_RS12780 (PSHAa2596) - 2742523..2743416 (+) 894 WP_011329244.1 hypothetical protein -
  PSHA_RS12785 (PSHAa2597) - 2743484..2743963 (+) 480 WP_011329245.1 hypothetical protein -
  PSHA_RS12790 (PSHAa2598) rsmE 2744036..2744770 (+) 735 WP_011329246.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PSHA_RS12795 (PSHAa2599) gshB 2744825..2745775 (+) 951 WP_011329247.1 glutathione synthase -
  PSHA_RS12800 (PSHAa2600) - 2745916..2746473 (+) 558 WP_011329248.1 YqgE/AlgH family protein -
  PSHA_RS12805 (PSHAa2601) ruvX 2746486..2746935 (+) 450 WP_011329249.1 Holliday junction resolvase RuvX -
  PSHA_RS12810 (PSHAa2602) pilU 2747002..2748138 (-) 1137 WP_011329250.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PSHA_RS12815 (PSHAa2603) pilT 2748148..2749188 (-) 1041 WP_041454575.1 type IV pilus twitching motility protein PilT Machinery gene
  PSHA_RS12820 (PSHAa2604) - 2749191..2749904 (+) 714 WP_011329252.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSHA_RS12825 (PSHAa2605) proC 2749922..2750743 (+) 822 WP_011329253.1 pyrroline-5-carboxylate reductase -
  PSHA_RS12830 (PSHAa2606) - 2750756..2751289 (+) 534 WP_011329254.1 YggT family protein -
  PSHA_RS12835 (PSHAa2607) - 2751348..2751791 (+) 444 WP_011329255.1 DUF4426 domain-containing protein -
  PSHA_RS12840 (PSHAa2608) rdgB 2751799..2752398 (+) 600 WP_041454577.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41839.84 Da        Isoelectric Point: 5.8295

>NTDB_id=24798 PSHA_RS12810 WP_011329250.1 2747002..2748138(-) (pilU) [Pseudoalteromonas translucida]
MALSLNNFLQIMIDKKGSDLFVSSQLPISAKINGELTPLDDEPLSDEQSLELVESAMSEKQKAEFHTTKECNFAIATDEG
RFRVSAFWQRDKAGMVIRRIVTKIPEIGELGLPSTLTDVIMSKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHRKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIIAQQLIPTSDGQGRVAAIEILLNSPMVAELIKNGDIGGIKETMAKSKEMGMQTFDQ
ALFELYKQQRINYADALHHADSANDLRLMIKLRNNEQQGAGFLQGVTIDGLDDKGNIS

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=24798 PSHA_RS12810 WP_011329250.1 2747002..2748138(-) (pilU) [Pseudoalteromonas translucida]
ATGGCTTTATCTTTAAATAACTTTTTACAAATAATGATAGATAAAAAAGGCTCTGATTTATTTGTTTCAAGCCAACTGCC
TATCAGCGCTAAAATTAATGGTGAATTAACCCCGCTTGATGATGAGCCGCTCAGCGATGAGCAATCACTTGAGTTGGTTG
AATCGGCAATGAGTGAAAAACAAAAAGCCGAGTTTCATACAACGAAAGAATGCAACTTTGCGATTGCCACTGACGAAGGA
CGTTTTCGTGTTTCGGCATTTTGGCAGCGCGATAAAGCGGGCATGGTTATTCGTCGTATTGTTACTAAAATACCTGAAAT
TGGCGAACTTGGGCTGCCGTCTACACTTACCGATGTGATTATGTCGAAACGTGGTTTAGTGCTATTTGTGGGGGGAACCG
GTACGGGTAAGTCAACCTCACTTGCTGCACTTATTGGCTATAGAAACCGTAATCAGCGCGGACATATATTAACCATTGAA
GACCCGATTGAGTTTGTACACGAACACCGTAAAAGTATTATTACTCAACGCGAAGTAGGGCTAGATACCGAAAGTTTTGA
GTCAGCGCTTAAAAGCTCGTTACGCCAAGCACCCGACGTAATATTAATAGGCGAAATACGCTCACAAGAAACCATGGAGT
ATGCATTAAGCTTTGCAGAAACAGGGCACTTATGTGTGGCTACCTTGCATGCAAACAACGCTAACCAGGCGATAGATCGG
ATCATGCATTTAGTGCCAAAAGAAAAGCACGATAAGCTTAAGTACGATCTAGCATTAAATTTACGCGCTATTATTGCCCA
GCAGCTGATCCCCACATCTGACGGGCAAGGTCGAGTTGCTGCTATTGAAATATTGCTTAATTCACCTATGGTTGCAGAGC
TAATTAAAAATGGTGATATAGGCGGTATTAAAGAAACCATGGCTAAATCGAAAGAAATGGGCATGCAGACCTTTGATCAG
GCCTTATTTGAGTTGTATAAACAGCAACGAATCAATTATGCAGATGCATTGCACCATGCCGATTCGGCGAACGATTTGCG
CTTAATGATAAAGCTGCGCAACAATGAGCAGCAAGGGGCGGGCTTTTTACAAGGGGTCACTATTGATGGCCTTGATGATA
AAGGCAATATTAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R4ISF5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.6

99.206

0.571
  pilU Acinetobacter baylyi ADP1

56.764

99.735

0.566
  pilU Vibrio cholerae strain A1552

57.766

97.09

0.561
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.044

95.503

0.421

Multiple sequence alignment