Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CNO08_RS05900 Genome accession   NZ_CP023465
Coordinates   1492958..1494124 (+) Length   388 a.a.
NCBI ID   WP_046655750.1    Uniprot ID   -
Organism   Lysobacter capsici strain KNU-14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1487958..1499124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CNO08_RS05875 (CNO08_05875) - 1488822..1489295 (-) 474 WP_096413805.1 DUF4426 domain-containing protein -
  CNO08_RS05880 (CNO08_05880) proC 1489292..1490128 (-) 837 WP_057920929.1 pyrroline-5-carboxylate reductase -
  CNO08_RS05885 (CNO08_05885) - 1490174..1490869 (+) 696 WP_157753843.1 hypothetical protein -
  CNO08_RS05890 (CNO08_05890) - 1490938..1491624 (-) 687 WP_269769285.1 YggS family pyridoxal phosphate-dependent enzyme -
  CNO08_RS05895 (CNO08_05895) pilT 1491815..1492852 (+) 1038 WP_036109068.1 type IV pilus twitching motility protein PilT Machinery gene
  CNO08_RS05900 (CNO08_05900) pilU 1492958..1494124 (+) 1167 WP_046655750.1 PilT/PilU family type 4a pilus ATPase Machinery gene

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43133.57 Da        Isoelectric Point: 6.8286

>NTDB_id=247232 CNO08_RS05900 WP_046655750.1 1492958..1494124(+) (pilU) [Lysobacter capsici strain KNU-14]
MNTPAPTNPTTASGAIDFTSFLKLMAHQRASDLFITSGMPPSMKVHGKISPITQNPLTPQQSRDLVLNVMNPQQREEFEK
THECNFAIGVTGIGRFRVSCFYQRNQVGMVLRRIETKIPTVEELNLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIG
YRNLNSTGHIITIEDPIEFVHRHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVL
CTLHANNANQAMDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLAQDYIRDGEIHKLKEL
MKDSVQLGMKTFDQALFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDARTLSQGLDGVEVAEVR

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=247232 CNO08_RS05900 WP_046655750.1 1492958..1494124(+) (pilU) [Lysobacter capsici strain KNU-14]
ATGAATACCCCGGCCCCGACCAACCCGACCACCGCCTCCGGCGCGATCGATTTCACCTCCTTCCTCAAGCTGATGGCGCA
CCAGCGCGCCTCGGACTTGTTCATCACCTCGGGCATGCCGCCGTCGATGAAGGTCCACGGCAAGATCAGCCCGATCACCC
AGAACCCGTTGACGCCGCAGCAAAGCCGCGACCTCGTGCTCAACGTGATGAACCCGCAGCAGCGCGAAGAGTTCGAAAAG
ACCCACGAGTGCAACTTCGCCATCGGCGTGACCGGCATCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGT
CGGCATGGTGCTGCGCCGGATCGAGACCAAGATCCCGACCGTCGAAGAACTGAACCTGCCGCCGATCATCAAGACGCTGG
CGATGACCAAGCGCGGCATCATCCTGTTCGTCGGCGCGACCGGCACCGGCAAGTCGACCTCGCTCGCGGCGATGATCGGC
TACCGCAATCTGAACTCGACCGGCCACATCATCACCATCGAAGATCCGATCGAATTCGTGCATCGCCACGAAGGCTGCAT
CATCACCCAGCGCGAAGTCGGCATCGACACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACG
TGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCATGGACCACGCCATCGCCTTCGCCGAAACCGGTCACCTGGTGCTG
TGCACGCTGCACGCCAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCGGAAGACCGTCGCAACCAGTT
GCTGATGGATTTGTCGTTGAACCTCAAGGGCGTGGTCGCGCAGCAGTTGATCCCGACCCCCGACGGCAAGGGCCGCCGCG
TCGCCATGGAAATCCTGCTCGGCACCCCGCTGGCCCAGGACTACATCCGCGACGGCGAAATCCACAAGCTCAAAGAGCTG
ATGAAGGACTCCGTGCAGCTCGGCATGAAGACCTTCGACCAGGCCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTA
CGAAGACGCCCTGCGCTACGCCGACTCGCAAAACGAAGTGCGCCTGCGCATCAAGCTGGCGCAAGGCGGCGATGCGCGGA
CTTTGTCGCAGGGGTTGGATGGGGTTGAGGTGGCTGAGGTCAGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(130-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.755

94.588

0.66
  pilU Acinetobacter baylyi ADP1

63.866

92.01

0.588
  pilU Vibrio cholerae strain A1552

53.652

91.753

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.165

90.464

0.381

Multiple sequence alignment