Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   CMR26_RS04530 Genome accession   NZ_CP023414
Coordinates   866709..867779 (+) Length   356 a.a.
NCBI ID   WP_012117985.1    Uniprot ID   A0A411A7V3
Organism   Bacillus velezensis strain BS-37     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 861709..872779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CMR26_RS04500 (CMR26_04495) - 862183..862428 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  CMR26_RS04505 (CMR26_04500) - 862665..863045 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  CMR26_RS04515 (CMR26_04510) - 863252..864082 (+) 831 WP_015240214.1 STAS domain-containing protein -
  CMR26_RS04520 (CMR26_04515) - 864120..865421 (-) 1302 WP_108724822.1 hemolysin family protein -
  CMR26_RS04525 (CMR26_04520) - 865567..866517 (+) 951 WP_032870601.1 magnesium transporter CorA family protein -
  CMR26_RS04530 (CMR26_04525) comGA 866709..867779 (+) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  CMR26_RS04535 (CMR26_04530) comGB 867766..868803 (+) 1038 WP_032866436.1 competence type IV pilus assembly protein ComGB Machinery gene
  CMR26_RS04540 (CMR26_04535) comGC 868850..869116 (+) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  CMR26_RS04545 (CMR26_04540) comGD 869106..869543 (+) 438 WP_012117983.1 competence type IV pilus minor pilin ComGD Machinery gene
  CMR26_RS04550 (CMR26_04545) comGE 869527..869841 (+) 315 WP_007408323.1 competence type IV pilus minor pilin ComGE -
  CMR26_RS04555 (CMR26_04550) comGF 869750..870250 (+) 501 WP_258566475.1 competence type IV pilus minor pilin ComGF -
  CMR26_RS04560 (CMR26_04555) comGG 870251..870628 (+) 378 WP_108724823.1 competence type IV pilus minor pilin ComGG Machinery gene
  CMR26_RS04565 (CMR26_04560) - 870685..870864 (+) 180 WP_003153093.1 YqzE family protein -
  CMR26_RS04570 (CMR26_04565) - 870904..871233 (-) 330 WP_012117979.1 DUF3889 domain-containing protein -
  CMR26_RS04575 (CMR26_04570) tapA 871492..872163 (+) 672 WP_085342189.1 amyloid fiber anchoring/assembly protein TapA -
  CMR26_RS04580 (CMR26_04575) sipW 872135..872704 (+) 570 WP_085342188.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.86 Da        Isoelectric Point: 9.4784

>NTDB_id=246868 CMR26_RS04530 WP_012117985.1 866709..867779(+) (comGA) [Bacillus velezensis strain BS-37]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=246868 CMR26_RS04530 WP_012117985.1 866709..867779(+) (comGA) [Bacillus velezensis strain BS-37]
GTGAAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment