Detailed information NTG0246581

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: CMV25_RS01510
• Genome accession: NZ_CP023392
• Coordinates: 312714..314966 (-)
• Length: 750 a.a.
• NCBI ID: WP_244165980.1
• Uniprot ID: -
• Organism: Lactococcus raffinolactis strain WiKim0068
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 307714..319966

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CMV25_RS01480 (CMV25_01480)	atpA	307971..309476 (-)	1506	WP_061773979.1	F0F1 ATP synthase subunit alpha	-
CMV25_RS01485 (CMV25_01485)	-	309617..310144 (-)	528	WP_061773978.1	F0F1 ATP synthase subunit delta	-
CMV25_RS01490 (CMV25_01490)	atpF	310147..310656 (-)	510	WP_061773977.1	F0F1 ATP synthase subunit B	-
CMV25_RS01495 (CMV25_01495)	atpB	310669..311379 (-)	711	WP_096039368.1	F0F1 ATP synthase subunit A	-
CMV25_RS01500 (CMV25_01500)	-	311485..311685 (-)	201	WP_003140734.1	F0F1 ATP synthase subunit C	-
CMV25_RS01505 (CMV25_01505)	-	311841..312635 (-)	795	WP_096039369.1	alpha/beta hydrolase family protein	-
CMV25_RS01510 (CMV25_01510)	comEC	312714..314966 (-)	2253	WP_244165980.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CMV25_RS01515 (CMV25_01515)	-	314932..315585 (-)	654	WP_244165981.1	helix-hairpin-helix domain-containing protein	-
CMV25_RS01520 (CMV25_01520)	-	315643..316863 (-)	1221	WP_157738471.1	ABC transporter permease	-
CMV25_RS01525 (CMV25_01525)	-	316856..317749 (-)	894	WP_061773972.1	ABC transporter ATP-binding protein	-
CMV25_RS01530 (CMV25_01530)	-	317924..318358 (-)	435	WP_096039372.1	hypothetical protein	-
CMV25_RS01535 (CMV25_01535)	-	318414..318608 (-)	195	WP_061773970.1	helix-turn-helix transcriptional regulator	-

Sequence

Protein

• Length: 750 a.a.
• Molecular weight: 85562.65 Da
• Isoelectric Point: 10.1062

>NTDB_id=246581 CMV25_RS01510 WP_244165980.1 312714..314966(-) (comEC) [Lactococcus raffinolactis strain WiKim0068]
MKNLKKALRWINLPSIFIAYFCVLIYYTVFCFSYLLLGYLIISLLILIWQNGIRTCLKLSMILIGFATICLALQDREAKD
FAREMTVTRISPILDTIQVDGDQVSFRGKSGKQTYQVYYRVTTEQEQRYFKQLAQPVVLSISGQLEKASQQRNFNGFDYQ
HHLKTQNIYRVLTIEQVTGVTRKQTLTLALLRQKAILFSEQHFPRPMSAYMTGLLFGHLGKDFEDMSSIYTSLGIMHLFA
LSGMQVSFFIDFLRKGMLRLGFRRDVVDWFQIPFSVFYAGLTGFSISVNRSLVQKILANFGIKALDNFSLTLLLLFITAP
KFLLTTGGTLSLLFAFVISIFGDRFENLPKYRKLLAESLTLSLCVLPLLILYFHTFQPVSIILTFVFSFLFDILFLPGLS
VIFLLAMVTGFTLTQVNLIFQWLEDLIKLTDSWWHYPLVLGKPTALVFLVTLVVTGFLIDHWQNRKVSARLAIILMGLFF
ITKNPPTPSITMVDVGQGYSIFLQDKFNRRSILIDTGGRIELGKKKKWQERKIAAPAEKTLIPYLKSRGVGQIDTLVLTH
THEDHMGDLLAVIDQIKVKNIIVSQGSLNNAQFVKLLKQTRAKVQVAKVGQRLKIFDSYLEVLYPLTPGDGKNNDSIVLY
GKLYGTKFLLTGDLEETGERELLTRYPNLSVDVLKVGHHGSKTSSSESFIEAIRPKVGLISCGLDNRYGHPNMETLTTFQ
KYEVQTFRTDYHGAIKFKKNSKFWHISTVK

Nucleotide

• Length: 2253 bp

>NTDB_id=246581 CMV25_RS01510 WP_244165980.1 312714..314966(-) (comEC) [Lactococcus raffinolactis strain WiKim0068]
TTGAAAAACTTAAAGAAAGCATTACGGTGGATTAATCTACCATCGATTTTCATCGCCTATTTTTGTGTGCTGATCTACTA
TACCGTCTTTTGTTTCTCCTATTTACTTTTAGGTTATCTCATCATTTCTTTGCTAATCTTGATCTGGCAAAATGGCATCA
GAACTTGTTTAAAGCTCAGCATGATCTTAATTGGGTTTGCAACCATTTGTCTAGCGCTACAAGATCGTGAAGCTAAAGAT
TTTGCGCGTGAGATGACAGTGACACGGATCAGCCCCATACTGGATACGATTCAGGTGGATGGTGACCAGGTCTCTTTTCG
TGGGAAATCGGGCAAACAAACCTATCAAGTCTATTATAGGGTCACGACGGAACAAGAGCAGCGCTACTTTAAGCAATTAG
CACAACCAGTCGTCCTATCCATTTCAGGTCAACTAGAAAAAGCGAGTCAACAAAGAAATTTCAATGGCTTTGATTATCAA
CACCATCTCAAGACACAAAATATCTATCGAGTTTTGACGATTGAACAGGTGACAGGGGTGACAAGAAAACAAACCTTAAC
CTTAGCGCTCTTACGACAAAAAGCTATTTTGTTTAGTGAGCAACACTTTCCGCGACCTATGTCAGCCTACATGACCGGTT
TACTTTTTGGGCATCTCGGTAAAGATTTTGAAGATATGAGCAGTATCTATACCAGCTTAGGCATCATGCACCTCTTTGCG
CTATCAGGCATGCAAGTGTCATTTTTTATCGACTTTTTAAGAAAGGGTATGCTCAGACTAGGGTTCAGGAGAGATGTTGT
CGATTGGTTTCAAATCCCTTTTTCTGTTTTTTATGCTGGTCTTACAGGATTTTCCATATCTGTCAACAGATCCTTGGTCC
AGAAAATCCTAGCTAATTTTGGAATTAAGGCGCTTGACAATTTCAGTCTCACGTTGTTGCTTCTATTTATCACGGCGCCA
AAATTCCTTTTAACCACAGGCGGCACCTTGTCTTTACTTTTTGCCTTCGTCATTTCAATCTTTGGCGATCGCTTTGAGAA
CCTCCCAAAATATCGTAAACTACTAGCAGAAAGTTTAACCCTTTCTCTATGCGTTTTACCGCTCCTCATCCTTTATTTTC
ACACCTTCCAACCAGTGTCGATCATCTTGACCTTTGTCTTCAGCTTTCTTTTTGATATTCTCTTCCTACCAGGTCTATCC
GTCATTTTCTTGCTAGCAATGGTAACAGGCTTCACGCTAACACAAGTCAATCTCATTTTTCAGTGGCTTGAAGACCTTAT
CAAATTGACAGATAGCTGGTGGCATTATCCGTTAGTACTTGGTAAACCAACAGCCTTGGTCTTTCTGGTGACGCTAGTTG
TGACAGGTTTTTTAATTGACCACTGGCAAAATCGAAAAGTTAGTGCTAGGCTGGCAATCATCTTGATGGGCTTATTCTTC
ATAACTAAAAATCCACCGACTCCTTCGATAACCATGGTGGATGTTGGCCAGGGGTATAGTATTTTCTTACAAGATAAGTT
TAATCGACGCAGTATTTTAATCGATACAGGTGGCAGGATTGAACTAGGTAAGAAGAAAAAGTGGCAGGAGCGTAAAATTG
CAGCACCAGCTGAAAAGACTTTAATTCCTTATTTGAAAAGTCGGGGTGTGGGTCAGATTGATACTTTGGTTCTAACCCAT
ACTCACGAGGACCATATGGGTGACCTACTCGCTGTCATCGACCAAATTAAGGTCAAAAATATCATCGTGAGTCAAGGCAG
TCTCAATAATGCCCAATTTGTCAAACTCTTAAAGCAAACCCGAGCTAAGGTTCAAGTGGCCAAGGTCGGTCAACGACTAA
AGATTTTTGACAGCTATTTGGAAGTTCTGTATCCATTGACCCCAGGAGATGGTAAAAATAATGACTCGATCGTCTTGTAT
GGTAAGCTCTATGGTACAAAATTTTTGTTAACAGGTGATTTGGAGGAGACAGGCGAGCGAGAGTTGTTGACACGTTATCC
CAATCTCTCAGTTGATGTTTTAAAAGTAGGGCATCATGGCTCAAAAACGTCATCTTCTGAGTCTTTTATTGAAGCGATTC
GACCAAAAGTCGGATTGATTTCTTGTGGTTTGGATAATCGGTACGGTCATCCAAATATGGAAACTCTCACAACCTTCCAG
AAATATGAGGTTCAAACTTTTCGAACAGATTATCATGGTGCTATAAAATTTAAGAAAAACAGTAAATTTTGGCACATCTC
GACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Competence protein (Competence) (214-463)

• Domain of unknown function (DUF4131) (DUF4131) (34-178)

• Metallo-beta-lactamase superfamily (Lactamase_B) (494-703)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Lactococcus lactis subsp. cremoris KW2	51.552	98.8	0.509
comEC/celB	Streptococcus mitis NCTC 12261	46.817	100	0.471
comEC/celB	Streptococcus mitis SK321	46.235	100	0.467
comEC/celB	Streptococcus pneumoniae TIGR4	45.358	100	0.456
comEC/celB	Streptococcus pneumoniae Rx1	44.562	100	0.448
comEC/celB	Streptococcus pneumoniae D39	44.562	100	0.448
comEC/celB	Streptococcus pneumoniae R6	44.562	100	0.448