Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   CLI97_RS02945 Genome accession   NZ_CP023320
Coordinates   539194..539631 (-) Length   145 a.a.
NCBI ID   WP_025852922.1    Uniprot ID   -
Organism   Bacillus velezensis strain SCGB     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 534194..544631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLI97_RS02895 (CLI97_00585) sinI 534579..534752 (+) 174 WP_003153105.1 anti-repressor SinI Regulator
  CLI97_RS02900 (CLI97_00586) sinR 534786..535121 (+) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  CLI97_RS02905 (CLI97_00587) tasA 535169..535954 (-) 786 WP_094247735.1 biofilm matrix protein TasA -
  CLI97_RS02910 (CLI97_00588) sipW 536018..536602 (-) 585 WP_012117977.1 signal peptidase I SipW -
  CLI97_RS02915 (CLI97_00589) tapA 536574..537245 (-) 672 WP_070082109.1 amyloid fiber anchoring/assembly protein TapA -
  CLI97_RS02920 (CLI97_00590) - 537504..537833 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  CLI97_RS02925 (CLI97_00591) - 537873..538052 (-) 180 WP_003153093.1 YqzE family protein -
  CLI97_RS02930 (CLI97_00592) comGG 538109..538486 (-) 378 WP_094247734.1 competence type IV pilus minor pilin ComGG Machinery gene
  CLI97_RS02935 (CLI97_00593) comGF 538487..538987 (-) 501 WP_227005764.1 competence type IV pilus minor pilin ComGF -
  CLI97_RS02940 (CLI97_00594) comGE 538896..539210 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  CLI97_RS02945 (CLI97_00595) comGD 539194..539631 (-) 438 WP_025852922.1 competence type IV pilus minor pilin ComGD Machinery gene
  CLI97_RS02950 (CLI97_00596) comGC 539621..539887 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  CLI97_RS02955 (CLI97_00597) comGB 539934..540971 (-) 1038 WP_063174751.1 competence type IV pilus assembly protein ComGB Machinery gene
  CLI97_RS02960 (CLI97_00598) comGA 540958..542028 (-) 1071 WP_070082113.1 competence type IV pilus ATPase ComGA Machinery gene
  CLI97_RS02965 (CLI97_00599) - 542220..543170 (-) 951 WP_071391609.1 magnesium transporter CorA family protein -
  CLI97_RS02970 (CLI97_00600) - 543316..544617 (+) 1302 WP_094247732.1 hemolysin family protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16268.78 Da        Isoelectric Point: 10.1850

>NTDB_id=246012 CLI97_RS02945 WP_025852922.1 539194..539631(-) (comGD) [Bacillus velezensis strain SCGB]
MNNNRRTENGFTLLESLIVLSLASVLLTVLFTTVPPVCTHLAVRQKTEQLQKDIQLAQETAIAEHKRTKITFLPKEHKYK
LQSAGRIVERSFDSLHITLVTLPESLEFNEKGHPNSGGKIQLKSAGFTYEITVYLGSGNVHAKRK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=246012 CLI97_RS02945 WP_025852922.1 539194..539631(-) (comGD) [Bacillus velezensis strain SCGB]
TTGAACAATAACAGGCGGACAGAAAACGGGTTTACCCTTCTTGAAAGCCTGATTGTGTTAAGCCTGGCGTCCGTCCTGCT
GACGGTTTTGTTCACGACGGTTCCGCCGGTCTGCACCCACCTTGCCGTGCGGCAAAAAACAGAACAGCTTCAAAAAGATA
TTCAGCTTGCGCAGGAAACGGCTATCGCAGAACATAAAAGAACAAAAATCACTTTTCTTCCAAAAGAGCATAAATACAAA
CTGCAGTCAGCCGGAAGGATTGTCGAGCGTTCTTTTGATTCGCTTCACATTACACTTGTGACTTTACCGGAGAGCCTTGA
ATTTAACGAAAAAGGCCATCCGAATTCGGGAGGAAAGATTCAATTGAAGAGCGCCGGGTTCACCTATGAGATCACAGTTT
ACTTAGGGAGCGGAAATGTCCATGCTAAACGGAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Bacillus subtilis subsp. subtilis str. 168

54.795

100

0.552

Multiple sequence alignment