Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   CMV05_RS13840 Genome accession   NZ_CP023310
Coordinates   2933705..2934307 (-) Length   200 a.a.
NCBI ID   WP_081245065.1    Uniprot ID   -
Organism   Vibrio anguillarum strain VIB12 isolate Sea bass     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2928705..2939307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CMV05_RS13815 (CMV05_13815) aroB 2929051..2930139 (-) 1089 WP_081245062.1 3-dehydroquinate synthase -
  CMV05_RS13820 (CMV05_13820) aroK 2930164..2930685 (-) 522 WP_010317691.1 shikimate kinase AroK -
  CMV05_RS13825 (CMV05_13825) pilQ 2930872..2932587 (-) 1716 WP_096036693.1 type IV pilus secretin PilQ family protein Machinery gene
  CMV05_RS13830 (CMV05_13830) pilP 2932611..2933132 (-) 522 WP_013855888.1 pilus assembly protein PilP Machinery gene
  CMV05_RS13835 (CMV05_13835) pilO 2933119..2933712 (-) 594 WP_013855887.1 type 4a pilus biogenesis protein PilO Machinery gene
  CMV05_RS13840 (CMV05_13840) pilN 2933705..2934307 (-) 603 WP_081245065.1 PilN domain-containing protein Machinery gene
  CMV05_RS13845 (CMV05_13845) pilM 2934297..2935307 (-) 1011 WP_081245066.1 type IV pilus assembly protein PilM Machinery gene
  CMV05_RS13850 (CMV05_13850) - 2935457..2937955 (+) 2499 WP_096036694.1 PBP1A family penicillin-binding protein -
  CMV05_RS13855 (CMV05_13855) oxyR 2937992..2938882 (-) 891 WP_026027425.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 200 a.a.        Molecular weight: 22649.12 Da        Isoelectric Point: 9.6928

>NTDB_id=245880 CMV05_RS13840 WP_081245065.1 2933705..2934307(-) (pilN) [Vibrio anguillarum strain VIB12 isolate Sea bass]
MLHSINLLPWREEQRAAHKRRFMGLVVLGVMSAVGIQWGIGLYFSGQAQQQESRLAYLNQYIQQLDKQINALKMTEQEHK
ALLTRLSVVESLQSQRNKTTELMNLMPQLMPEGVYVDKIKMNGEEIEITGISDSTARLATMLDNFEKSALLSDVNMHSIV
HGNKRFGKEFQTFKVSFMFHSPTPQGAALTKIRSTGGVHD

Nucleotide


Download         Length: 603 bp        

>NTDB_id=245880 CMV05_RS13840 WP_081245065.1 2933705..2934307(-) (pilN) [Vibrio anguillarum strain VIB12 isolate Sea bass]
ATGTTGCATAGCATTAACTTACTGCCTTGGCGAGAGGAACAAAGAGCGGCGCATAAGCGTCGTTTTATGGGCTTGGTCGT
CTTAGGTGTGATGAGCGCTGTTGGTATTCAATGGGGTATCGGTTTGTATTTCTCTGGGCAAGCACAGCAGCAAGAGTCTC
GTCTGGCCTATTTGAATCAATACATTCAGCAGCTTGATAAACAGATCAATGCATTAAAAATGACGGAACAAGAACACAAG
GCACTATTAACTCGCTTATCGGTTGTTGAGTCATTGCAAAGCCAACGCAATAAAACCACCGAGTTGATGAACCTGATGCC
ACAGCTTATGCCTGAAGGAGTCTACGTCGATAAGATCAAAATGAATGGTGAAGAAATTGAAATTACGGGCATCAGTGACA
GCACTGCGCGTCTTGCGACCATGCTCGATAATTTTGAAAAATCAGCCTTACTTTCCGATGTCAACATGCACTCTATCGTG
CACGGTAATAAGCGATTTGGTAAGGAGTTTCAGACGTTTAAAGTGTCATTTATGTTTCATTCGCCGACACCACAGGGCGC
TGCACTAACCAAGATTCGGTCTACAGGAGGTGTCCATGACTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

64

100

0.64
  pilN Vibrio campbellii strain DS40M4

65.775

93.5

0.615

Multiple sequence alignment