Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CLM74_RS17205 Genome accession   NZ_CP023271
Coordinates   3760395..3761453 (-) Length   352 a.a.
NCBI ID   WP_032951624.1    Uniprot ID   A0AA46AYB7
Organism   Stenotrophomonas sp. MYb57     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3755395..3766453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLM74_RS17185 (CLM74_17115) - 3756312..3758291 (-) 1980 WP_105164580.1 type IV pilus secretin PilQ family protein -
  CLM74_RS17190 (CLM74_17120) - 3758313..3758819 (-) 507 WP_051501657.1 pilus assembly protein PilP -
  CLM74_RS17195 (CLM74_17125) pilO 3758846..3759502 (-) 657 WP_025874231.1 type 4a pilus biogenesis protein PilO -
  CLM74_RS17200 (CLM74_17130) - 3759499..3760395 (-) 897 WP_105164581.1 PilN domain-containing protein -
  CLM74_RS17205 (CLM74_17135) pilM 3760395..3761453 (-) 1059 WP_032951624.1 pilus assembly protein PilM Machinery gene
  CLM74_RS17210 (CLM74_17140) - 3761671..3764097 (+) 2427 WP_105164582.1 penicillin-binding protein 1A -
  CLM74_RS17215 (CLM74_17145) - 3764409..3765053 (+) 645 WP_059064965.1 hypothetical protein -
  CLM74_RS17220 (CLM74_17150) - 3765115..3765852 (-) 738 WP_032977004.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37695.33 Da        Isoelectric Point: 4.4047

>NTDB_id=245658 CLM74_RS17205 WP_032951624.1 3760395..3761453(-) (pilM) [Stenotrophomonas sp. MYb57]
MGLIPKSQSPLIGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAMNRSGSKAKLAAAA
VAGSAVITKVIPMPADLDENDMEAQIELEAVNYIPYPIEEVNLDFEVIGAIPNNPEMVQVLLAASRSENVELRQSALELG
GLQAKVMDVEAFAVENAFALVASELPVSAEGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEIMRRYGLSYEE
AGLAKRQGGLPESYEMEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAALAGLPEMVEEQLGVPTVVANPLA
QMTLGPKVQAHALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=245658 CLM74_RS17205 WP_032951624.1 3760395..3761453(-) (pilM) [Stenotrophomonas sp. MYb57]
GTGGGGCTCATCCCAAAAAGTCAGTCGCCGCTCATTGGCGTCGACATCAGTTCGACTGCGGTAAAGCTGTTGCAGCTTTC
CCGCAGCGGCAACCGTTTCCGTGTGGAACATTATGCCGTGGAACCGCTGCCGCCGAATGCGGTGGTGGAGAAGAACATCG
TTGAAGTGGAGGCCGTGGGAGAGGCCATCCGCCGGGCGATGAACCGTTCCGGCAGCAAGGCCAAGCTGGCCGCTGCGGCC
GTGGCCGGTTCGGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGACCTGGACGAGAACGACATGGAAGCCCAGATCGA
GCTGGAAGCGGTCAACTACATTCCGTATCCGATCGAGGAAGTGAACCTGGACTTCGAGGTGATCGGGGCGATCCCGAACA
ACCCGGAAATGGTCCAGGTGCTGCTGGCTGCCTCGCGTTCGGAAAATGTGGAACTGCGCCAGTCCGCACTGGAACTGGGT
GGCCTGCAGGCCAAGGTGATGGACGTGGAGGCCTTCGCGGTCGAGAACGCCTTCGCCCTGGTGGCCAGCGAGCTGCCGGT
TTCGGCCGAGGGCGTGGTTGCGCTGGTCGATATCGGCGCCACCATGACCACCCTCAACGTACTGCGCGGTGGCCGTAGCC
TGTACAGCCGCGAACAGGTGTTCGGTGGCAAGCAGCTGACCGACGAGATCATGCGCCGTTATGGCCTGAGCTACGAGGAA
GCCGGTCTGGCCAAGCGCCAGGGCGGGTTGCCGGAAAGCTACGAGATGGAAGTGCTGGAGCCGTTCAAGGAAGCCACGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTACGCCGGCAGCGAGTTCAACCGCGTCGATCACATCGTGCTGGCCGGCG
GCTGTGCCGCCCTTGCAGGCCTGCCGGAGATGGTCGAGGAACAGCTGGGTGTGCCGACCGTGGTCGCCAACCCGCTGGCA
CAGATGACCCTGGGGCCGAAGGTGCAGGCGCACGCGCTGGCCCAGGACGCCCCCGCGCTGATGATCGCGACCGGTCTGGC
GCTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.989

100

0.52
  comM Acinetobacter nosocomialis M2

51.989

100

0.52
  comM Acinetobacter baylyi ADP1

50.284

100

0.503
  pilM Legionella pneumophila strain ERS1305867

47.293

99.716

0.472

Multiple sequence alignment