Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   CK945_RS15475 Genome accession   NZ_CP023168
Coordinates   2973718..2974347 (-) Length   209 a.a.
NCBI ID   WP_096748238.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain 14DA11     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2968718..2979347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CK945_RS15455 holA 2969277..2970302 (-) 1026 WP_167511161.1 DNA polymerase III subunit delta -
  CK945_RS15460 - 2970532..2970663 (+) 132 WP_003183686.1 YqzM family protein -
  CK945_RS15465 - 2970688..2973075 (-) 2388 WP_096748237.1 DNA internalization-related competence protein ComEC/Rec2 -
  CK945_RS15470 - 2973080..2973649 (-) 570 WP_020452273.1 ComE operon protein 2 -
  CK945_RS15475 comEA 2973718..2974347 (-) 630 WP_096748238.1 helix-hairpin-helix domain-containing protein Machinery gene
  CK945_RS15480 comER 2974430..2975251 (+) 822 WP_229128976.1 late competence protein ComER -
  CK945_RS15485 - 2975322..2976077 (-) 756 WP_023855255.1 class I SAM-dependent DNA methyltransferase -
  CK945_RS15490 rsfS 2976074..2976430 (-) 357 WP_096748240.1 ribosome silencing factor -
  CK945_RS15495 yqeK 2976444..2977007 (-) 564 WP_096748241.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  CK945_RS15500 - 2976997..2977566 (-) 570 WP_025811125.1 nicotinate-nucleotide adenylyltransferase -
  CK945_RS15505 yhbY 2977585..2977875 (-) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  CK945_RS15510 aroE 2977869..2978705 (-) 837 WP_096748242.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22628.43 Da        Isoelectric Point: 4.6492

>NTDB_id=245121 CK945_RS15475 WP_096748238.1 2973718..2974347(-) (comEA) [Bacillus paralicheniformis strain 14DA11]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSGFSIPEEASAQTFDKKEEVKREKNAGEEEVIIDLKGAVKNPGV
YQMKEGDRVHDALKKAGGTEKKADQKQINLAAVLRDGMVLYIPFEGEEAAGSLSEADSRADGSSSDIVNINTASSEELQT
IPGIGPSKAEAVVEYREENGMFQTIEDITNVSGIGEKSFERIKSSITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=245121 CK945_RS15475 WP_096748238.1 2973718..2974347(-) (comEA) [Bacillus paralicheniformis strain 14DA11]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCGGCGGGAGGTGTGGCACTCGTCTTGATCATCAGCGCTGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCCGGCTTCTCCATTCCTGAAGAGGCTTCTGCACAGACTTTTGACAAGAAAG
AGGAGGTGAAGCGGGAAAAAAACGCGGGGGAAGAGGAGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGTGTT
TATCAAATGAAGGAGGGAGACAGGGTGCACGATGCATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGACCAAAAGCA
AATTAATCTGGCAGCCGTTTTGCGGGACGGTATGGTTCTTTACATTCCATTTGAAGGGGAAGAGGCCGCTGGCTCTCTTT
CGGAAGCGGACTCAAGAGCAGACGGCAGCTCAAGTGATATCGTCAATATCAATACGGCTTCCTCTGAGGAGCTTCAGACG
ATTCCCGGGATCGGTCCTTCAAAAGCGGAAGCGGTAGTCGAATACCGCGAGGAGAACGGAATGTTTCAGACGATTGAAGA
CATAACAAACGTTTCTGGAATTGGAGAGAAGTCTTTTGAAAGAATAAAATCTTCAATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(69-122)

Helix-hairpin-helix motif (HHH_3)

(145-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

52.336

100

0.536
  comEA Staphylococcus aureus MW2

36.364

100

0.383
  comEA Staphylococcus aureus N315

35.909

100

0.378
  comEA Lactococcus lactis subsp. cremoris KW2

35.023

100

0.364

Multiple sequence alignment