Detailed information NTG0244895

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CK824_RS14270
• Genome accession: NZ_CP023140
• Coordinates: 2956171..2957289 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain XH906
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2951171..2962289

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CK824_RS14260 (CK824_14225)	-	2954287..2954979 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
CK824_RS14265 (CK824_14230)	pilT	2955106..2956143 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
CK824_RS14270 (CK824_14235)	pilU	2956171..2957289 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CK824_RS14275 (CK824_14240)	fur	2957359..2957796 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
CK824_RS14280 (CK824_14245)	-	2957908..2958306 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
CK824_RS14285 (CK824_14250)	-	2958361..2958684 (-)	324	WP_000442587.1	RnfH family protein	-
CK824_RS14290 (CK824_14255)	-	2958684..2959751 (-)	1068	WP_002019572.1	hypothetical protein	-
CK824_RS14300 (CK824_14265)	-	2959904..2960359 (-)	456	WP_000782976.1	bacteriohemerythrin	-
CK824_RS14305 (CK824_14270)	-	2960612..2961460 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=244895 CK824_RS14270 WP_000347039.1 2956171..2957289(+) (pilU) [Acinetobacter baumannii strain XH906]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=244895 CK824_RS14270 WP_000347039.1 2956171..2957289(+) (pilU) [Acinetobacter baumannii strain XH906]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395