Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   M28_RS00685 Genome accession   NC_007296
Coordinates   104441..104875 (+) Length   144 a.a.
NCBI ID   WP_002992738.1    Uniprot ID   -
Organism   Streptococcus pyogenes MGAS6180     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99441..109875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M28_RS00655 (M28_Spy0083) - 101110..101475 (+) 366 WP_002986560.1 DUF1033 family protein -
  M28_RS00660 (M28_Spy0084) comYA 101568..102506 (+) 939 WP_011284420.1 competence type IV pilus ATPase ComGA Machinery gene
  M28_RS00665 (M28_Spy0085) comYB 102442..103476 (+) 1035 WP_227868694.1 competence type IV pilus assembly protein ComGB Machinery gene
  M28_RS00670 (M28_Spy0086) comYC 103478..103804 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  M28_RS00675 (M28_Spy0087) comGD 103779..104207 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  M28_RS00680 (M28_Spy0088) comGE 104164..104448 (+) 285 WP_011284422.1 competence type IV pilus minor pilin ComGE -
  M28_RS00685 (M28_Spy0089) comYF 104441..104875 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  M28_RS00690 (M28_Spy0090) comGG 104859..105185 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  M28_RS00695 (M28_Spy0091) comYH 105283..106236 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  M28_RS00700 (M28_Spy0092) - 106295..107491 (+) 1197 WP_011284424.1 acetate kinase -
  M28_RS00705 (M28_Spy0093) - 107678..107986 (+) 309 WP_011284425.1 hypothetical protein -
  M28_RS00710 (M28_Spy0094) proC 108069..108839 (-) 771 WP_011284426.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16645.27 Da        Isoelectric Point: 10.2526

>NTDB_id=24464 M28_RS00685 WP_002992738.1 104441..104875(+) (comYF) [Streptococcus pyogenes MGAS6180]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=24464 M28_RS00685 WP_002992738.1 104441..104875(+) (comYF) [Streptococcus pyogenes MGAS6180]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

44.776

93.056

0.417
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396

Multiple sequence alignment