Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CK207_RS13605 Genome accession   NZ_CP023054
Coordinates   2904487..2905497 (-) Length   336 a.a.
NCBI ID   WP_019282438.1    Uniprot ID   A0A289GF81
Organism   Vibrio anguillarum strain VIB43     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2899487..2910497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CK207_RS13580 (CK207_13580) aroK 2900354..2900875 (-) 522 WP_010317691.1 shikimate kinase AroK -
  CK207_RS13585 (CK207_13585) pilQ 2901062..2902777 (-) 1716 WP_019282437.1 type IV pilus secretin PilQ family protein Machinery gene
  CK207_RS13590 (CK207_13590) pilP 2902801..2903322 (-) 522 WP_013855888.1 pilus assembly protein PilP Machinery gene
  CK207_RS13595 (CK207_13595) pilO 2903309..2903902 (-) 594 WP_013855887.1 type 4a pilus biogenesis protein PilO Machinery gene
  CK207_RS13600 (CK207_13600) pilN 2903895..2904497 (-) 603 WP_017047271.1 PilN domain-containing protein Machinery gene
  CK207_RS13605 (CK207_13605) pilM 2904487..2905497 (-) 1011 WP_019282438.1 type IV pilus assembly protein PilM Machinery gene
  CK207_RS13610 (CK207_13610) - 2905647..2908145 (+) 2499 WP_019282439.1 PBP1A family penicillin-binding protein -
  CK207_RS13615 (CK207_13615) oxyR 2908182..2909072 (-) 891 WP_026027425.1 DNA-binding transcriptional regulator OxyR -
  CK207_RS13620 (CK207_13620) - 2909219..2909947 (+) 729 WP_013855882.1 redoxin family protein -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37427.96 Da        Isoelectric Point: 7.5395

>NTDB_id=244621 CK207_RS13605 WP_019282438.1 2904487..2905497(-) (pilM) [Vibrio anguillarum strain VIB43]
MGKSLVTGIDIGHHSIKAVVLKLAGDSYALVGYKELFITEDIFSDNHTLDYQKIVKKLKELRKGLPLFSRKVAISVPDNA
VISKVLQIDSDLQAREKEFAIYQAFSHQSPFPIEELSLDFVKVMEKSVSRSSTVSYQVYATRKDVVESRALAAQKAGFLP
IAVDVQIHSLLSIWQLASRAQHRSDWMLMDVGHTQTALCIDFTDKAPFHKEIPLGCQWLSQDSAMSGTNAELADKNERFV
HELIERLQRQLQLFTSVNGAGSLAGIWLSGGGADTPMLAQEISRRLKLECEILDPFLLFNNKLAKRQRDKQSGQSFSTAA
GLALRGIEWLESSHVA

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=244621 CK207_RS13605 WP_019282438.1 2904487..2905497(-) (pilM) [Vibrio anguillarum strain VIB43]
ATGGGTAAATCATTGGTTACGGGTATAGATATTGGCCACCATAGTATTAAAGCGGTGGTGTTAAAGCTAGCGGGTGATTC
ATATGCACTCGTCGGTTATAAAGAATTATTTATTACTGAGGACATATTCTCCGATAACCACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAGAAAGGGTTTACCACTATTTAGTCGCAAAGTCGCCATATCGGTACCGGACAATGCG
GTAATAAGTAAGGTATTACAGATAGATAGTGACCTGCAGGCGCGTGAAAAAGAGTTTGCTATTTATCAGGCATTTTCTCA
TCAGTCACCGTTCCCGATAGAAGAACTGAGTCTCGATTTCGTCAAAGTGATGGAAAAAAGCGTAAGTCGCAGTAGCACCG
TAAGTTATCAAGTTTACGCCACTCGTAAAGACGTCGTGGAAAGCCGTGCGTTAGCGGCTCAAAAAGCGGGCTTTTTACCG
ATCGCGGTTGATGTGCAAATACACAGTTTGTTAAGCATCTGGCAGTTGGCGAGCAGGGCACAACATCGCAGCGATTGGAT
GCTCATGGATGTGGGCCATACCCAAACGGCGCTCTGTATTGATTTTACTGATAAAGCCCCTTTTCATAAAGAGATCCCTC
TCGGTTGTCAGTGGTTGTCCCAAGACAGCGCGATGTCGGGCACCAATGCGGAATTAGCCGATAAAAACGAGCGCTTTGTG
CATGAGTTGATCGAGCGACTTCAACGTCAGTTGCAGCTTTTCACCTCCGTCAATGGCGCGGGCAGTTTAGCAGGCATTTG
GCTCTCAGGTGGTGGCGCAGATACTCCGATGTTGGCGCAAGAGATCTCGCGGCGTCTCAAGCTTGAGTGCGAGATTCTCG
ATCCCTTCCTTCTGTTTAACAATAAGTTAGCGAAACGCCAGCGAGATAAGCAGTCTGGGCAGAGTTTTAGCACCGCGGCT
GGCCTTGCACTGCGTGGTATCGAGTGGTTGGAGTCTTCTCATGTTGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A289GF81

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

67.456

100

0.679
  pilM Vibrio campbellii strain DS40M4

52.976

100

0.53

Multiple sequence alignment