Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   CKG20_RS03740 Genome accession   NZ_CP023047
Coordinates   730312..731625 (-) Length   437 a.a.
NCBI ID   WP_006247937.1    Uniprot ID   A0A248ZXM0
Organism   Mannheimia haemolytica strain USDA-ARS-USMARC-186     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 725312..736625
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKG20_RS03710 (CKG20_03710) ruvB 725809..726813 (-) 1005 WP_006247944.1 Holliday junction branch migration DNA helicase RuvB -
  CKG20_RS03715 (CKG20_03715) ruvA 726950..727555 (-) 606 WP_006247943.1 Holliday junction branch migration protein RuvA -
  CKG20_RS03720 (CKG20_03720) - 727667..728290 (-) 624 WP_006247941.1 LysE family transporter -
  CKG20_RS03725 (CKG20_03725) - 728297..728770 (-) 474 WP_006247940.1 phosphatidylglycerophosphatase A -
  CKG20_RS03730 (CKG20_03730) thiL 728775..729755 (-) 981 WP_006247939.1 thiamine-phosphate kinase -
  CKG20_RS03735 (CKG20_03735) nusB 729818..730231 (-) 414 WP_006247938.1 transcription antitermination factor NusB -
  CKG20_RS03740 (CKG20_03740) comE 730312..731625 (-) 1314 WP_006247937.1 type IV pilus secretin PilQ Machinery gene
  CKG20_RS03745 (CKG20_03745) - 731634..732047 (-) 414 WP_006247936.1 hypothetical protein -
  CKG20_RS03750 (CKG20_03750) - 732050..732586 (-) 537 WP_006247935.1 hypothetical protein -
  CKG20_RS03755 (CKG20_03755) - 732588..733109 (-) 522 WP_006247934.1 hypothetical protein -
  CKG20_RS03760 (CKG20_03760) - 733085..733783 (-) 699 WP_006247933.1 hypothetical protein -
  CKG20_RS03765 (CKG20_03765) - 733907..736471 (+) 2565 WP_006247932.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48502.53 Da        Isoelectric Point: 8.6222

>NTDB_id=244473 CKG20_RS03740 WP_006247937.1 730312..731625(-) (comE) [Mannheimia haemolytica strain USDA-ARS-USMARC-186]
MWRIFIYFLCGFSLAYANTISIVLKNAPTSLIFTYLAEETGKNMVLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQ
EDDIVYIHKKEEKAADLTPLPIMNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGHYKFGGRLEGNGLNNVTNNLNVN
FPITGGASAVLQVSSINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKSSNRKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=244473 CKG20_RS03740 WP_006247937.1 730312..731625(-) (comE) [Mannheimia haemolytica strain USDA-ARS-USMARC-186]
ATGTGGCGAATTTTTATATATTTCTTATGCGGTTTTTCATTAGCCTATGCGAATACCATTTCCATAGTTTTAAAAAATGC
TCCAACTTCGCTTATTTTTACTTATTTAGCGGAAGAAACCGGTAAAAATATGGTGCTTGATGATAATATTGAAACCAAAT
CAACACTACGATTAGAAAATAAATCGGTTGACGAGATTTTTAAAACGCTTGGCAAAGTCAATAAATTATCCTTAACGCAA
GAAGATGATATTGTTTATATTCACAAAAAAGAGGAAAAAGCGGCTGACTTAACGCCTCTGCCAATAATGAATTTGCAAAA
TAATGGGCAAAATACACCGCTTGTCACCGCCCCTAAGTTAATCACAAAAACCTTGAAACTACATTATGCAAAGGCTTCAG
AGGTGATTGAGTCGTTAACCAAAGGCAGCGGAACGTTTTTATCGGAAAACGGTTATATTCACTTTGATGAAAGAAGCAAT
AGCCTGATTGTGAAAGACAGTGCAAAATCGCTTAAAAATATCGAGAATTTAGTAAAACAGCTCGACCAACCCACCGAGCA
AATTGCGATTGAGGCTAGAATTGTCACTATCAGTAGCGAGCATTTGCAAGAGCTTGGCGTTCGCTGGGGAATTTTTTCTC
GTGGGGCTGGGCACTATAAATTTGGCGGACGGCTGGAAGGCAATGGACTCAACAATGTCACCAATAACTTAAATGTAAAT
TTTCCGATTACTGGTGGAGCATCTGCCGTATTACAGGTTTCGTCCATTAATAGCAGAGTGCTTGATTTAGAATTAAGTGC
ATTAGAACAAGAAAATAGTGTGGAAATTATTGCCAGCCCACGCCTGTTAACCACCAATAAAAAGCCGGCAAGTATCAAGC
AAGGCACGGAAATTCCTTATGTGATGTACAACACCAAATCGGAAGCCACCGATGTCGAATTTAAAGAAGCGGTATTAGGG
CTTGAAGTCGTTCCACACCTTTCAACCCAAAATCAAATTCTACTCGATCTGATCGTTACCCAAAATTCGCCAAATTCCCA
ATCCGGCAGTAGTGGCTTAATTACGATTGATAAACAAGAGCTAAATACGCAAGTATTTGCCAAACACGGCGAAACCATTG
TACTTGGTGGTATTTTTCAACATTTAACCCAAAAAGGCGAAGATAAAGTGCCGATTTTAGGCTCAATTCCCTTCATCAAG
CGGTTATTTAGTCAAACTCGAGATAAAATCAGCAAAAGAGAGCTAGTCATTTTTGTGACGCCTTATATCCTTCAATCTAA
TGAAAAGAGCAGCAACCGCAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)

Bacterial type II and III secretion system protein (Secretin)

(267-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A248ZXM0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

62.844

99.771

0.627
  comE Haemophilus influenzae Rd KW20

51.034

99.542

0.508
  comE Haemophilus influenzae 86-028NP

50.345

99.542

0.501
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.717

96.339

0.373
  pilQ Vibrio cholerae strain A1552

38.717

96.339

0.373
  pilQ Vibrio campbellii strain DS40M4

37.383

97.941

0.366

Multiple sequence alignment