Detailed information NTG0244253

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Aba9102_RS06080
• Genome accession: NZ_CP023029
• Coordinates: 1223716..1224834 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain 9102
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1218716..1229834

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Aba9102_RS06070 (Aba9102_06060)	-	1221832..1222524 (-)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-
Aba9102_RS06075 (Aba9102_06065)	pilT	1222651..1223688 (+)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
Aba9102_RS06080 (Aba9102_06070)	pilU	1223716..1224834 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
Aba9102_RS06085 (Aba9102_06075)	fur	1224904..1225341 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
Aba9102_RS06090 (Aba9102_06080)	-	1225453..1225851 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
Aba9102_RS06095 (Aba9102_06085)	-	1225906..1226229 (-)	324	WP_000442585.1	RnfH family protein	-
Aba9102_RS06100 (Aba9102_06090)	-	1226229..1227296 (-)	1068	WP_000092403.1	hypothetical protein	-
Aba9102_RS06110 (Aba9102_06100)	-	1227449..1227904 (-)	456	WP_000782976.1	bacteriohemerythrin	-
Aba9102_RS06115 (Aba9102_06105)	-	1228157..1229005 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=244253 Aba9102_RS06080 WP_000347036.1 1223716..1224834(+) (pilU) [Acinetobacter baumannii strain 9102]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=244253 Aba9102_RS06080 WP_000347036.1 1223716..1224834(+) (pilU) [Acinetobacter baumannii strain 9102]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392