Detailed information NTG0244153

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Aba9201_RS18245
• Genome accession: NZ_CP023020
• Coordinates: 3675139..3676257 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347041.1
• Uniprot ID: A0A3R9E3W9
• Organism: Acinetobacter baumannii strain 9201
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3670139..3681257

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Aba9201_RS18210 (Aba9201_18190)	-	3670901..3671869 (+)	969	WP_014538385.1	IS30 family transposase	-
Aba9201_RS18215 (Aba9201_18195)	-	3672069..3672524 (+)	456	WP_000782976.1	bacteriohemerythrin	-
Aba9201_RS18225 (Aba9201_18205)	-	3672677..3673744 (+)	1068	WP_029642282.1	membrane protein	-
Aba9201_RS18230 (Aba9201_18210)	-	3673744..3674067 (+)	324	WP_000442585.1	RnfH family protein	-
Aba9201_RS18235 (Aba9201_18215)	-	3674122..3674520 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
Aba9201_RS18240 (Aba9201_18220)	fur	3674632..3675069 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
Aba9201_RS18245 (Aba9201_18225)	pilU	3675139..3676257 (-)	1119	WP_000347041.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
Aba9201_RS18250 (Aba9201_18230)	pilT	3676285..3677322 (-)	1038	WP_000355487.1	type IV pilus twitching motility protein PilT	Machinery gene
Aba9201_RS18255 (Aba9201_18235)	-	3677449..3678141 (+)	693	WP_118896106.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41922.14 Da
• Isoelectric Point: 6.5789

>NTDB_id=244153 Aba9201_RS18245 WP_000347041.1 3675139..3676257(-) (pilU) [Acinetobacter baumannii strain 9201]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQYLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=244153 Aba9201_RS18245 WP_000347041.1 3675139..3676257(-) (pilU) [Acinetobacter baumannii strain 9201]
ATGGATTTTAATGACTTGCTCAATCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGTATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (29-270)

3D structure

• AlphaFold DB: A0A3R9E3W9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	68.194	99.731	0.68
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392