Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   VF_RS11915 Genome accession   NC_006840
Coordinates   2577937..2578515 (-) Length   192 a.a.
NCBI ID   WP_011262702.1    Uniprot ID   Q5E2F6
Organism   Aliivibrio fischeri ES114     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2572937..2583515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VF_RS11895 (VF2291) aroB 2573783..2574871 (-) 1089 WP_011262699.1 3-dehydroquinate synthase -
  VF_RS11900 (VF2292) aroK 2574908..2575426 (-) 519 WP_005421091.1 shikimate kinase AroK -
  VF_RS11905 (VF2293) pilQ 2575651..2577339 (-) 1689 WP_047863557.1 type IV pilus secretin PilQ Machinery gene
  VF_RS11910 (VF2294) pilP 2577426..2577944 (-) 519 WP_011262701.1 pilus assembly protein PilP Machinery gene
  VF_RS11915 (VF2295) pilO 2577937..2578515 (-) 579 WP_011262702.1 type 4a pilus biogenesis protein PilO Machinery gene
  VF_RS11920 (VF2296) - 2578515..2579081 (-) 567 WP_011262703.1 PilN domain-containing protein -
  VF_RS11925 (VF2297) pilM 2579066..2580067 (-) 1002 WP_011262704.1 type IV pilus biogenesis protein PilM -
  VF_RS11930 (VF2298) - 2580202..2582700 (+) 2499 WP_011262705.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 22477.89 Da        Isoelectric Point: 4.8892

>NTDB_id=24270 VF_RS11915 WP_011262702.1 2577937..2578515(-) (pilO) [Aliivibrio fischeri ES114]
MDWRELDLDEITEWPIIPQLLVILLLCLCIQGAGYWFWLQPIQESIEQSKLDEISLKTTVKYKYNQVAAMPKMKDQLNEL
NLRYEFLSQQLPAQKELASMLSGINQVGIQNKLTFTRIDWGEKQNKTFLYKLPLNIELTGQYHDIGSFSEAMADLPRIVS
LENIDIQRVSKESSTLHFRVMAYTYQFRGDHE

Nucleotide


Download         Length: 579 bp        

>NTDB_id=24270 VF_RS11915 WP_011262702.1 2577937..2578515(-) (pilO) [Aliivibrio fischeri ES114]
ATGGATTGGCGTGAATTAGATTTAGATGAAATTACGGAATGGCCGATTATTCCGCAGCTGTTAGTGATCTTGTTGTTGTG
TTTATGTATTCAAGGGGCTGGATACTGGTTTTGGTTACAACCAATACAAGAAAGTATCGAGCAGTCAAAATTGGATGAAA
TAAGTTTAAAAACAACGGTGAAATATAAATATAACCAAGTTGCTGCTATGCCTAAAATGAAAGATCAATTGAATGAATTG
AATCTACGATATGAGTTTTTATCGCAGCAGCTACCCGCTCAAAAAGAGTTAGCAAGCATGCTTTCGGGTATCAACCAAGT
TGGTATCCAAAATAAGCTCACTTTTACTCGGATTGACTGGGGGGAGAAGCAAAATAAAACCTTTTTATATAAGTTACCTC
TGAATATTGAGCTGACAGGGCAATACCACGACATCGGCTCGTTTTCTGAAGCCATGGCTGATTTACCGCGTATTGTTAGT
TTGGAAAATATAGATATTCAAAGGGTAAGTAAGGAGAGTAGTACATTGCACTTTCGTGTAATGGCTTATACCTACCAGTT
TAGGGGGGACCATGAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(46-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5E2F6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

62.162

96.354

0.599
  pilO Vibrio campbellii strain DS40M4

57.447

97.917

0.563

Multiple sequence alignment