Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   CJZ71_RS03165 Genome accession   NZ_CP022891
Coordinates   544782..545852 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis strain DKU_NT_03     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 539782..550852
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJZ71_RS03120 (CJZ71_03120) tapA 540261..541022 (-) 762 WP_014480251.1 amyloid fiber anchoring/assembly protein TapA -
  CJZ71_RS03125 (CJZ71_03125) yqzG 541294..541620 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  CJZ71_RS03130 (CJZ71_03130) spoIITA 541662..541841 (-) 180 WP_014480252.1 YqzE family protein -
  CJZ71_RS03135 (CJZ71_03135) comGG 541912..542286 (-) 375 WP_069837632.1 ComG operon protein ComGG Machinery gene
  CJZ71_RS03140 (CJZ71_03140) comGF 542287..542670 (-) 384 WP_029317913.1 ComG operon protein ComGF Machinery gene
  CJZ71_RS03145 (CJZ71_03145) comGE 542696..543043 (-) 348 WP_014480255.1 ComG operon protein 5 Machinery gene
  CJZ71_RS03150 (CJZ71_03150) comGD 543027..543458 (-) 432 WP_014480256.1 comG operon protein ComGD Machinery gene
  CJZ71_RS03155 (CJZ71_03155) comGC 543448..543744 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  CJZ71_RS03160 (CJZ71_03160) comGB 543758..544795 (-) 1038 WP_031600685.1 comG operon protein ComGB Machinery gene
  CJZ71_RS03165 (CJZ71_03165) comGA 544782..545852 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  CJZ71_RS03170 (CJZ71_03170) - 546064..546261 (-) 198 WP_014480259.1 CBS domain-containing protein -
  CJZ71_RS03175 (CJZ71_03175) corA 546263..547216 (-) 954 WP_014480260.1 magnesium transporter CorA -
  CJZ71_RS03180 (CJZ71_03180) yqhB 547358..548686 (+) 1329 WP_014480261.1 hemolysin family protein -
  CJZ71_RS03185 (CJZ71_03185) rsbRD 548739..549575 (-) 837 WP_014480262.1 RsbT co-antagonist protein RsbRD -
  CJZ71_RS03195 (CJZ71_03195) mgsR 549799..550179 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  CJZ71_RS03200 (CJZ71_03200) yqgY 550411..550656 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -
  CJZ71_RS22300 - 550657..550797 (+) 141 WP_155118966.1 hypothetical protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=242610 CJZ71_RS03165 WP_004399124.1 544782..545852(-) (comGA) [Bacillus subtilis strain DKU_NT_03]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=242610 CJZ71_RS03165 WP_004399124.1 544782..545852(-) (comGA) [Bacillus subtilis strain DKU_NT_03]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAAGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATTGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGTCGCCAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGGAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment