Detailed information    

insolico Bioinformatically predicted

Overview


Name   comEA   Type   Machinery gene
Locus tag   CJZ70_RS07405 Genome accession   NZ_CP022890
Coordinates   1440044..1440661 (+) Length   205 a.a.
NCBI ID   WP_046160608.1    Uniprot ID   -
Organism   Bacillus subtilis strain DKU_NT_02     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1404861..1443631 1440044..1440661 within 0


Gene organization within MGE regions


Location: 1404861..1443631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJZ70_RS07205 (CJZ70_07205) czcR 1404861..1405727 (-) 867 WP_014480355.1 LysR family transcriptional regulator CzcR -
  CJZ70_RS07210 (CJZ70_07210) yrdR 1405853..1406818 (+) 966 WP_014480354.1 DMT family transporter -
  CJZ70_RS07215 (CJZ70_07215) yrzO 1406836..1406979 (+) 144 WP_014477437.1 YrzO family protein -
  CJZ70_RS07220 (CJZ70_07220) yrkA 1407269..1408573 (+) 1305 WP_019712547.1 hemolysin family protein -
  CJZ70_RS07225 (CJZ70_07225) - 1408819..1410066 (+) 1248 WP_031600262.1 IS256-like element ISBsu2 family transposase -
  CJZ70_RS07230 (CJZ70_07230) - 1410501..1410791 (-) 291 WP_014480337.1 contact-dependent growth inhibition system immunity protein -
  CJZ70_RS07235 (CJZ70_07235) istA 1410951..1412498 (+) 1548 WP_014480339.1 IS21 family transposase -
  CJZ70_RS07240 (CJZ70_07240) istB 1412495..1413253 (+) 759 WP_014479891.1 IS21-like element helper ATPase IstB -
  CJZ70_RS07245 (CJZ70_07245) atxG 1413387..1413963 (-) 577 Protein_1425 suppressor of fused domain protein -
  CJZ70_RS07250 (CJZ70_07250) - 1414231..1414464 (-) 234 WP_224588641.1 hypothetical protein -
  CJZ70_RS21155 - 1414553..1414756 (+) 204 WP_123772462.1 hypothetical protein -
  CJZ70_RS07255 (CJZ70_07255) - 1415064..1415576 (-) 513 WP_014477426.1 hypothetical protein -
  CJZ70_RS07260 (CJZ70_07260) cdiI 1415648..1416007 (-) 360 WP_014480334.1 ribonuclease toxin immunity protein CdiI -
  CJZ70_RS07265 (CJZ70_07265) - 1416104..1416556 (-) 453 WP_014480333.1 SMI1/KNR4 family protein -
  CJZ70_RS07270 (CJZ70_07270) - 1416655..1417095 (-) 441 WP_014480332.1 SMI1/KNR4 family protein -
  CJZ70_RS07275 (CJZ70_07275) - 1417498..1417785 (-) 288 WP_014480331.1 hypothetical protein -
  CJZ70_RS21735 (CJZ70_07280) - 1417799..1419725 (-) 1927 Protein_1433 T7SS effector LXG polymorphic toxin -
  CJZ70_RS07285 (CJZ70_07285) - 1419907..1421034 (+) 1128 WP_014480328.1 Rap family tetratricopeptide repeat protein -
  CJZ70_RS07295 (CJZ70_07295) - 1421773..1422168 (-) 396 WP_046160622.1 VOC family protein -
  CJZ70_RS07300 (CJZ70_07300) - 1423109..1423999 (-) 891 WP_014480326.1 LysR family transcriptional regulator -
  CJZ70_RS07305 (CJZ70_07305) fumC 1424166..1425554 (+) 1389 WP_014480325.1 class II fumarate hydratase -
  CJZ70_RS07310 (CJZ70_07310) - 1425773..1425912 (+) 140 Protein_1438 recombinase family protein -
  CJZ70_RS07315 (CJZ70_07315) - 1426035..1427282 (+) 1248 WP_031600262.1 IS256-like element ISBsu2 family transposase -
  CJZ70_RS21160 - 1427359..1427457 (-) 99 WP_031600702.1 hypothetical protein -
  CJZ70_RS07320 (CJZ70_07320) sigK 1427457..1428185 (-) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  CJZ70_RS07325 (CJZ70_07325) nucA/comI 1428380..1428790 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  CJZ70_RS07330 (CJZ70_07330) yqeB 1428823..1429545 (-) 723 WP_014480321.1 hypothetical protein -
  CJZ70_RS07335 (CJZ70_07335) gnd 1429796..1430689 (+) 894 WP_069837643.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  CJZ70_RS07340 (CJZ70_07340) yqeD 1430708..1431334 (-) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  CJZ70_RS07345 (CJZ70_07345) cwlH 1431521..1432273 (+) 753 WP_069837642.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  CJZ70_RS07350 (CJZ70_07350) yqeF 1432525..1433256 (+) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  CJZ70_RS07355 (CJZ70_07355) - 1433562..1433702 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  CJZ70_RS07360 (CJZ70_07360) yqeG 1434064..1434581 (+) 518 Protein_1449 YqeG family HAD IIIA-type phosphatase -
  CJZ70_RS07365 (CJZ70_07365) yqeH 1434585..1435684 (+) 1100 Protein_1450 ribosome biogenesis GTPase YqeH -
  CJZ70_RS07370 (CJZ70_07370) aroE 1435702..1436544 (+) 843 WP_014480317.1 shikimate dehydrogenase -
  CJZ70_RS07375 (CJZ70_07375) yhbY 1436538..1436828 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  CJZ70_RS07380 (CJZ70_07380) nadD 1436840..1437409 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  CJZ70_RS07385 (CJZ70_07385) yqeK 1437399..1437959 (+) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  CJZ70_RS07390 (CJZ70_07390) rsfS 1437977..1438333 (+) 357 WP_014480315.1 ribosome silencing factor -
  CJZ70_RS07395 (CJZ70_07395) yqeM 1438330..1439073 (+) 744 WP_046160609.1 class I SAM-dependent methyltransferase -
  CJZ70_RS07400 (CJZ70_07400) comER 1439139..1439960 (-) 822 WP_032726195.1 late competence protein ComER -
  CJZ70_RS07405 (CJZ70_07405) comEA 1440044..1440661 (+) 618 WP_046160608.1 competence protein ComEA Machinery gene
  CJZ70_RS07410 (CJZ70_07410) comEB 1440728..1441297 (+) 570 WP_003229978.1 ComE operon protein 2 -
  CJZ70_RS07415 (CJZ70_07415) comEC 1441301..1443631 (+) 2331 WP_046160607.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene

Sequence


Protein


Download         Length: 205 a.a.        Molecular weight: 21738.48 Da        Isoelectric Point: 4.7284

>NTDB_id=242543 CJZ70_RS07405 WP_046160608.1 1440044..1440661(+) (comEA) [Bacillus subtilis strain DKU_NT_02]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=242543 CJZ70_RS07405 WP_046160608.1 1440044..1440661(+) (comEA) [Bacillus subtilis strain DKU_NT_02]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains


Predicted by InterproScan.

(63-118)

(142-203)


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976

  comEA Staphylococcus aureus MW2

35.909

100

0.385

  comEA Staphylococcus aureus N315

35

100

0.376


Multiple sequence alignment