Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   CHY23_RS10460 Genome accession   NZ_CP022715
Coordinates   2186127..2186573 (-) Length   148 a.a.
NCBI ID   WP_005597397.1    Uniprot ID   B0BPG5
Organism   Actinobacillus pleuropneumoniae strain KL 16     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2181127..2191573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHY23_RS10435 (CHY23_02113) yacG 2182034..2182222 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  CHY23_RS10440 (CHY23_02114) coaE 2182204..2182842 (-) 639 WP_005597390.1 dephospho-CoA kinase -
  CHY23_RS10445 (CHY23_02115) - 2182864..2183502 (-) 639 WP_005597392.1 prepilin peptidase -
  CHY23_RS10450 (CHY23_02116) pilC 2183529..2184731 (-) 1203 WP_005597394.1 type II secretion system F family protein Machinery gene
  CHY23_RS10455 (CHY23_02117) pilB 2184715..2186109 (-) 1395 WP_117374128.1 GspE/PulE family protein Machinery gene
  CHY23_RS10460 (CHY23_02118) pilA 2186127..2186573 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CHY23_RS10465 (CHY23_02119) radA 2186755..2188134 (+) 1380 WP_005597398.1 DNA repair protein RadA -
  CHY23_RS10470 (CHY23_02120) - 2188178..2188411 (-) 234 WP_005597399.1 YgjV family protein -
  CHY23_RS10475 (CHY23_02121) folA 2188538..2189026 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  CHY23_RS10480 (CHY23_02122) proB 2189113..2190204 (+) 1092 WP_005597401.1 glutamate 5-kinase -
  CHY23_RS10485 (CHY23_02123) - 2190217..2190993 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence


Protein


Download         Length: 148 a.a.        Molecular weight: 15980.38 Da        Isoelectric Point: 7.6257

>NTDB_id=241841 CHY23_RS10460 WP_005597397.1 2186127..2186573(-) (pilA) [Actinobacillus pleuropneumoniae strain KL 16]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=241841 CHY23_RS10460 WP_005597397.1 2186127..2186573(-) (pilA) [Actinobacillus pleuropneumoniae strain KL 16]
ATGCAAAAATTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACAGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB B0BPG5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689

  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547

  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527


Multiple sequence alignment