Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EBN1_RS03710 Genome accession   NC_006513
Coordinates   781273..782502 (-) Length   409 a.a.
NCBI ID   WP_011236570.1    Uniprot ID   Q5P769
Organism   Aromatoleum aromaticum EbN1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 776273..787502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EBN1_RS03680 (EB_RS03640) - 776761..777756 (+) 996 WP_041645680.1 KpsF/GutQ family sugar-phosphate isomerase -
  EBN1_RS03685 (EB_RS03645) - 777756..778277 (+) 522 WP_011236564.1 HAD family hydrolase -
  EBN1_RS03690 (EB_RS03650) lptC 778286..778867 (+) 582 WP_011236565.1 LPS export ABC transporter periplasmic protein LptC -
  EBN1_RS03695 (EB_RS03655) lptA 778864..779436 (+) 573 WP_011236566.1 lipopolysaccharide transport periplasmic protein LptA -
  EBN1_RS03700 (EB_RS03660) - 779487..780263 (+) 777 WP_011236567.1 enoyl-CoA hydratase -
  EBN1_RS03705 (EB_RS03665) - 780615..781175 (+) 561 WP_011236569.1 phasin family protein -
  EBN1_RS03710 (EB_RS03670) pilU 781273..782502 (-) 1230 WP_011236570.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EBN1_RS03715 (EB_RS03675) - 782611..783402 (-) 792 WP_011236571.1 lysophospholipid acyltransferase family protein -
  EBN1_RS03720 (EB_RS03680) gmhB 783411..783944 (-) 534 WP_011236572.1 D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase -
  EBN1_RS03725 (EB_RS03685) glyS 783960..786047 (-) 2088 WP_041646897.1 glycine--tRNA ligase subunit beta -
  EBN1_RS03730 (EB_RS03690) glyQ 786047..786967 (-) 921 WP_041645683.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 45089.43 Da        Isoelectric Point: 6.4035

>NTDB_id=24103 EBN1_RS03710 WP_011236570.1 781273..782502(-) (pilU) [Aromatoleum aromaticum EbN1]
MASALGPEEARNYMHKLLKAMHQMGGSDLFISADFPPSIKSHGGMKPLSQQRLAAEVTKSLALSLMNERQRREFEAEMEC
NFAVSLPDVCRFRVNVFIQQQQVGMVIRTIASEIPNLDKLDLPGVLKDVIMTKRGLVLVVGGTGSGKSTTLAAMIDHRNT
NSAGHIITVEDPVEYVHKNKGCLVTHREVGVDTHSWEHALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCLGTLH
ANNSNQTLDRIINFFPEERRNQLLMDLSSNLRAIVSQRLVRTEDGAGRKAAIEILLNTPTIAEMIFKGQFHSIKEIMAKS
RELGMCTFDQALFALYDAHHISYEEAIRNADSANELRLQIKLKAGRGQPSSGIGSEFSLSIEESVEEDSGEAEKAELLPA
SGASTAHTS

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=24103 EBN1_RS03710 WP_011236570.1 781273..782502(-) (pilU) [Aromatoleum aromaticum EbN1]
ATGGCATCCGCGCTCGGCCCTGAAGAAGCACGCAATTACATGCACAAGCTGCTGAAGGCGATGCACCAGATGGGAGGCTC
GGACCTTTTCATCTCGGCCGACTTCCCGCCGAGCATCAAGTCGCACGGCGGCATGAAACCGCTGAGCCAGCAGCGGCTCG
CTGCCGAAGTGACCAAGTCGCTCGCGCTGAGCCTGATGAACGAGCGCCAGCGGCGCGAATTCGAGGCCGAGATGGAGTGC
AATTTCGCCGTCTCGCTGCCCGACGTGTGCCGCTTCCGCGTCAACGTGTTCATCCAGCAACAGCAGGTCGGCATGGTGAT
CCGGACGATCGCCTCGGAAATTCCGAATCTCGACAAGCTCGACCTGCCCGGCGTGCTCAAGGACGTCATCATGACGAAGC
GCGGTCTGGTGCTTGTCGTGGGCGGCACGGGATCGGGCAAGTCGACGACACTGGCGGCGATGATCGACCACCGCAACACG
AATTCGGCCGGCCACATCATCACCGTCGAGGATCCGGTCGAGTACGTGCACAAGAACAAGGGCTGCCTCGTCACTCACCG
TGAAGTCGGCGTCGATACGCATTCTTGGGAGCACGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATCG
GCGAGATCCGCGATCGCGAGACGATGGAGCACGCCATTGCATTTGCCGAAACCGGGCACCTGTGTCTGGGGACGCTGCAC
GCGAACAATTCGAATCAGACGCTCGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCAACCAGCTCCTGATGGACCT
TTCGTCGAACCTGCGGGCGATCGTCTCGCAGCGCCTCGTCCGCACCGAGGACGGCGCCGGGCGCAAGGCCGCGATCGAAA
TCCTCCTGAACACCCCGACGATCGCCGAAATGATCTTCAAGGGACAGTTCCATTCGATCAAGGAGATCATGGCGAAGTCG
CGCGAACTGGGGATGTGCACGTTCGATCAGGCGCTGTTCGCCCTGTATGACGCGCACCACATCAGCTACGAAGAGGCGAT
CCGGAATGCCGACTCGGCGAACGAGTTGCGCCTGCAGATCAAGCTGAAGGCCGGCCGCGGGCAGCCGTCGTCGGGCATTG
GCAGCGAGTTCAGCCTGTCGATCGAGGAGTCCGTGGAGGAGGATTCAGGCGAAGCGGAAAAAGCGGAGCTGCTGCCCGCG
TCCGGCGCCTCGACGGCACACACGTCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(90-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5P769

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.942

84.841

0.509
  pilU Acinetobacter baylyi ADP1

55.085

86.553

0.477
  pilU Vibrio cholerae strain A1552

51.136

86.064

0.44
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.662

86.797

0.379

Multiple sequence alignment