Detailed information NTG0239316

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: Asalp_RS20650
• Genome accession: NZ_CP022426
• Coordinates: 4414045..4414920 (+)
• Length: 291 a.a.
• NCBI ID: WP_021140338.1
• Uniprot ID: T0QW70
• Organism: Aeromonas salmonicida subsp. pectinolytica 34mel
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4409045..4419920

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Asalp_RS20630 (Asalp_40380)	-	4409319..4409741 (+)	423	WP_021139037.1	pilin	-
Asalp_RS20635 (Asalp_40400)	-	4409861..4410892 (+)	1032	WP_001809438.1	IS630-like element ISAhy2 family transposase	-
Asalp_RS20640 (Asalp_40390)	pilB	4410929..4412611 (+)	1683	WP_034524152.1	PilB family type IVa pilus assembly ATPase TapB	Machinery gene
Asalp_RS20645 (Asalp_40410)	pilC	4412740..4413981 (+)	1242	WP_021140339.1	type II secretion system F family protein	Machinery gene
Asalp_RS20650 (Asalp_40420)	pilD	4414045..4414920 (+)	876	WP_021140338.1	A24 family peptidase	Machinery gene
Asalp_RS20655 (Asalp_40430)	coaE	4414940..4415554 (+)	615	WP_021140337.1	dephospho-CoA kinase	-
Asalp_RS20660 (Asalp_40440)	zapD	4415592..4416314 (+)	723	WP_021140336.1	cell division protein ZapD	-
Asalp_RS20665 (Asalp_40450)	yacG	4416324..4416518 (+)	195	WP_005314167.1	DNA gyrase inhibitor YacG	-
Asalp_RS20670 (Asalp_40460)	mutT	4416596..4417003 (-)	408	WP_005314168.1	8-oxo-dGTP diphosphatase MutT	-
Asalp_RS20675 (Asalp_40470)	secA	4417170..4419890 (-)	2721	WP_021140335.1	preprotein translocase subunit SecA	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 32550.84 Da
• Isoelectric Point: 7.0807

>NTDB_id=239316 Asalp_RS20650 WP_021140338.1 4414045..4414920(+) (pilD) [Aeromonas salmonicida subsp. pectinolytica 34mel]
MTLLLELAHSLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYRSYFSSDTPQPEADERYNLMVPRSCCPHCNH
PITALENIPLLSWLWLKGRCRSCHATISARYPLVELLTALLSVVVAMVITPGWGTLAALLLTWVLVALTFIDLDKMLLPD
QLTLPLLWSGLLFNLLGGYVPLGDAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSS
LVGAIFGIGLILLRNHHQSKPIPFGPYLAIAGWIALLWGDSITRWYLSTVL

Nucleotide

• Length: 876 bp

>NTDB_id=239316 Asalp_RS20650 WP_021140338.1 4414045..4414920(+) (pilD) [Aeromonas salmonicida subsp. pectinolytica 34mel]
ATGACACTTCTTCTCGAGCTGGCACACAGCTTGCCATGGCTCTACTTCTCGCTGGTATTTCTCTTCTCCCTGATGATCGG
CAGCTTTCTCAATGTGGTGATCCACCGCCTGCCCATCATGCTGGAACGGGAGTGGCAGGCCGAATATCGCAGCTATTTCA
GTTCAGACACGCCGCAACCAGAGGCTGACGAGCGCTACAATCTGATGGTACCGCGCTCCTGCTGCCCTCACTGCAATCAC
CCCATAACGGCACTGGAAAACATTCCCTTGCTGAGCTGGCTCTGGCTCAAAGGTCGCTGCCGGAGCTGCCATGCCACCAT
TTCGGCTCGCTATCCGCTGGTGGAGCTGCTGACCGCTCTGCTCTCTGTGGTGGTTGCCATGGTAATAACACCAGGCTGGG
GCACGCTGGCTGCTCTTCTGCTGACCTGGGTGCTGGTGGCGCTCACCTTTATCGATCTGGACAAGATGCTGCTGCCGGAC
CAGCTCACCTTGCCGCTGCTGTGGAGCGGGCTGCTGTTCAACCTGCTTGGCGGATACGTGCCTCTCGGCGATGCGGTCAT
CGGCGCCATGGCAGGCTATCTGGTGCTGTGGAGCCTCTACTGGGCCTTCAAGCTGCTTACCGGCAAGGAGGGCATGGGCT
ATGGGGACTTCAAACTGCTGGCGGCGCTGGGAGCCTGGCTTGGCTGGCAGGCGCTGCCCATAGTACTGCTGCTCTCTTCT
CTGGTCGGCGCCATCTTCGGCATTGGCCTCATTTTGCTGCGCAATCATCACCAGAGCAAACCCATCCCCTTTGGCCCCTA
TCTCGCCATCGCAGGCTGGATCGCGCTGCTCTGGGGCGATAGCATCACCCGCTGGTATCTCTCCACTGTCCTCTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-127)

• Type IV leader peptidase family (Peptidase_A24) (140-248)

3D structure

• AlphaFold DB: T0QW70

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	60.764	98.969	0.601
pilD	Vibrio campbellii strain DS40M4	54.861	98.969	0.543
pilD	Acinetobacter baumannii D1279779	53.929	96.22	0.519
pilD	Acinetobacter nosocomialis M2	53.929	96.22	0.519
pilD	Neisseria gonorrhoeae MS11	50.373	92.096	0.464