Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiA   Type   Regulator
Locus tag   STU_RS16130 Genome accession   NC_006448
Coordinates   1276242..1278209 (-) Length   655 a.a.
NCBI ID   WP_011225333.1    Uniprot ID   Q5M6C6
Organism   Streptococcus thermophilus LMG 18311     
Function   internalize XIP (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1256209..1326298 1276242..1278209 within 0
IScluster/Tn 1278565..1285928 1276242..1278209 flank 356


Gene organization within MGE regions


Location: 1256209..1326298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STU_RS16040 (stu1422) liaF 1256217..1256915 (-) 699 WP_011226289.1 cell wall-active antibiotics response protein LiaF -
  STU_RS20685 (stu1423) - 1257181..1257336 (-) 156 WP_011226290.1 ion channel -
  STU_RS16045 (stu1425) stkP/pknB 1257973..1259844 (-) 1872 WP_011226292.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  STU_RS16050 (stu1426) - 1259844..1260581 (-) 738 WP_002946881.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  STU_RS16055 (stu1427) rsmB 1260625..1261947 (-) 1323 WP_014608533.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  STU_RS16060 (stu1428) fmt 1261937..1262872 (-) 936 WP_002951411.1 methionyl-tRNA formyltransferase -
  STU_RS16065 (stu1429) - 1262890..1265286 (-) 2397 WP_011226295.1 primosomal protein N' -
  STU_RS16070 (stu1430) rpoZ 1265428..1265742 (-) 315 WP_002951415.1 DNA-directed RNA polymerase subunit omega -
  STU_RS16075 (stu1431) gmk 1265764..1266393 (-) 630 WP_011226296.1 guanylate kinase -
  STU_RS16080 (stu1432) ftsY 1266626..1268017 (-) 1392 WP_011226297.1 signal recognition particle-docking protein FtsY -
  STU_RS16085 (stu1433) - 1268031..1268846 (-) 816 WP_011226298.1 Cof-type HAD-IIB family hydrolase -
  STU_RS16090 (stu1434) - 1268839..1269633 (-) 795 WP_011226299.1 HAD hydrolase family protein -
  STU_RS16095 (stu1435) - 1269817..1270194 (+) 378 WP_011226300.1 GntR family transcriptional regulator -
  STU_RS16100 (stu1436) - 1270199..1270897 (+) 699 WP_011226301.1 ABC transporter ATP-binding protein -
  STU_RS16105 (stu1437) - 1270909..1271694 (+) 786 WP_002951425.1 hypothetical protein -
  STU_RS16110 (stu1438) amiF 1271744..1272673 (-) 930 WP_002951426.1 ATP-binding cassette domain-containing protein Regulator
  STU_RS16115 (stu1439) amiE 1272666..1273751 (-) 1086 WP_011226304.1 ABC transporter ATP-binding protein Regulator
  STU_RS16120 (stu1440) amiD 1273761..1274687 (-) 927 WP_002946409.1 oligopeptide ABC transporter permease OppC Regulator
  STU_RS16125 (stu1441) amiC 1274687..1276180 (-) 1494 WP_002946410.1 ABC transporter permease Regulator
  STU_RS16130 (stu1442) amiA 1276242..1278209 (-) 1968 WP_011225333.1 peptide ABC transporter substrate-binding protein Regulator
  STU_RS16135 (stu1444) - 1278565..1279814 (+) 1250 Protein_1243 ISL3 family transposase -
  STU_RS16140 (stu1445) amiA3 1279859..1281832 (-) 1974 WP_011226306.1 peptide ABC transporter substrate-binding protein Regulator
  STU_RS19635 - 1282036..1282226 (-) 191 Protein_1245 IS3 family transposase -
  STU_RS16145 (stu1447) - 1282220..1282630 (-) 411 Protein_1246 ATP-binding cassette domain-containing protein -
  STU_RS20240 - 1282662..1282976 (-) 315 Protein_1247 TatD family hydrolase -
  STU_RS16155 (stu1450) - 1283326..1284531 (+) 1206 WP_011226310.1 L-lactate MFS transporter -
  STU_RS19640 - 1284577..1285928 (-) 1352 Protein_1249 IS3 family transposase -
  STU_RS16175 (stu1455) pta 1286014..1286997 (-) 984 WP_011226315.1 phosphate acetyltransferase -
  STU_RS16180 (stu1456) - 1287011..1287907 (-) 897 WP_011226316.1 RluA family pseudouridine synthase -
  STU_RS16185 (stu1457) - 1287904..1288740 (-) 837 WP_041827075.1 NAD kinase -
  STU_RS16190 (stu1459) - 1288712..1289386 (-) 675 WP_002951443.1 GTP pyrophosphokinase -
  STU_RS16195 (stu1458) - 1289482..1290057 (+) 576 WP_002951444.1 CYTH domain-containing protein -
  STU_RS16200 (stu1460) - 1290422..1291393 (+) 972 WP_002951446.1 ribose-phosphate diphosphokinase -
  STU_RS16205 (stu1461) - 1291397..1292509 (+) 1113 WP_002948029.1 cysteine desulfurase family protein -
  STU_RS16210 (stu1462) - 1292511..1292858 (+) 348 WP_011226321.1 DUF1831 domain-containing protein -
  STU_RS16215 (stu1463) - 1293003..1293662 (+) 660 WP_041828288.1 redox-sensing transcriptional repressor Rex -
  STU_RS16220 (stu1464) - 1293655..1294350 (+) 696 WP_011226323.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
  STU_RS16225 (stu1465) radC 1294403..1295089 (-) 687 WP_011226324.1 RadC family protein -
  STU_RS16230 (stu1467) - 1295138..1296922 (-) 1785 WP_011226325.1 rhamnan synthesis F family protein -
  STU_RS16235 (stu1468) - 1296919..1297974 (-) 1056 WP_002948022.1 glycosyltransferase family 2 protein -
  STU_RS16240 (stu1469) - 1297994..1299199 (-) 1206 WP_011226326.1 ABC transporter ATP-binding protein -
  STU_RS16245 (stu1470) - 1299199..1300008 (-) 810 WP_002948020.1 ABC transporter permease -
  STU_RS16250 (stu1471) - 1299992..1300948 (-) 957 WP_011226328.1 glycosyltransferase family 2 protein -
  STU_RS16255 (stu1472) cps2T 1300945..1302093 (-) 1149 WP_041828290.1 beta 1-4 rhamnosyltransferase Cps2T -
  STU_RS16260 (stu1473) - 1302202..1303212 (-) 1011 WP_011226330.1 glycosyltransferase family 2 protein -
  STU_RS16265 (stu1480) - 1303212..1304486 (-) 1275 WP_011226331.1 lipopolysaccharide biosynthesis protein -
  STU_RS16270 (stu1481) - 1304479..1304811 (-) 333 WP_011226332.1 DUF2304 domain-containing protein -
  STU_RS16275 (stu1482) - 1304817..1305512 (-) 696 WP_173940715.1 glycosyltransferase family 2 protein -
  STU_RS16280 (stu1483) rfbD 1305589..1306440 (-) 852 WP_014621820.1 dTDP-4-dehydrorhamnose reductase -
  STU_RS16285 (stu1484) - 1306517..1307851 (-) 1335 WP_041828293.1 DUF3329 domain-containing protein -
  STU_RS16290 (stu1485) - 1307964..1308881 (-) 918 WP_002946425.1 glycosyltransferase family 2 protein -
  STU_RS16295 (stu1486) - 1308944..1310359 (-) 1416 WP_011226336.1 DUF2142 domain-containing protein -
  STU_RS16300 (stu1487) - 1310693..1311028 (-) 336 WP_002891474.1 metal-sulfur cluster assembly factor -
  STU_RS19995 (stu1488) rpoD 1311082..1312191 (-) 1110 WP_011226338.1 RNA polymerase sigma factor RpoD -
  STU_RS20000 (stu1489) dnaG 1312195..1314006 (-) 1812 WP_011226339.1 DNA primase -
  STU_RS16315 (stu1490) rpsU 1314377..1314553 (-) 177 WP_011226340.1 30S ribosomal protein S21 -
  STU_RS16320 (stu1492) - 1314748..1315542 (-) 795 WP_011226341.1 transporter substrate-binding domain-containing protein -
  STU_RS16325 (stu1493) - 1315635..1316744 (-) 1110 WP_041828295.1 aminotransferase -
  STU_RS16330 (stu1494) - 1317091..1317888 (-) 798 WP_011226343.1 transporter substrate-binding domain-containing protein -
  STU_RS16335 (stu1495) - 1317885..1318715 (-) 831 WP_041828297.1 transporter substrate-binding domain-containing protein -
  STU_RS16340 (stu1496) - 1318928..1319392 (-) 465 WP_011226345.1 8-oxo-dGTP diphosphatase -
  STU_RS16345 (stu1497) uvrB 1319437..1321428 (-) 1992 WP_002953358.1 excinuclease ABC subunit UvrB -
  STU_RS20995 - 1322115..1323051 (-) 937 Protein_1285 CPBP family intramembrane glutamic endopeptidase -
  STU_RS16365 (stu1501) - 1323250..1325460 (+) 2211 WP_011226350.1 ABC transporter substrate-binding protein/permease -
  STU_RS16370 (stu1502) - 1325460..1326200 (+) 741 WP_002945131.1 amino acid ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 655 a.a.        Molecular weight: 71760.87 Da        Isoelectric Point: 5.0409

>NTDB_id=23928 STU_RS16130 WP_011225333.1 1276242..1278209(-) (amiA) [Streptococcus thermophilus LMG 18311]
MKLKKILGITGVALLSVGMLAACSSKSSTSGTTYSNIYGSDPETLDYITSIMGGTKAVLTNGVDGLMEADKYGNLVPSVA
EDWSVSKDGLTYTYKIRKGIKWYTSEGEEYANVTAKDFVTGLKHAADAKSGALYLVQDSITGLSDYLSGANKDFSNVGVK
AIDDYTLQYTLKKQEPYWNSKTTYGVLFPVNEDFLKNKGKDFGKSTDPTSILYNGPFLLKSLTAKSSIELTKNENYWDKK
NVHFDSIKFSYSDGSDLESIERSFSDGALSIARVFPTSSNYASVEKKYKDNIFYTEPGASTSVIGVNIDRQSYKFSAKKT
DAEKTSTKKALLNKDFRQSINFAIDRTAYQSQVNGKDGAALALRNLFVPYDFVSAGDKTFGDLVTEKMSSYGDEWSGVNF
ADGQEGLYNAEKAKTEFAKAKEALQGEGVQFPVHLDLPVDQSSKLSVAQAQSLKQTIEKSLGSENVVIDINQMSSDDMNN
VTSNAANAAAEDWDISNGVGWGPDYQDPSTYLDILKTTSSENTKIFNGYDDPNNAAAAQVGLKDYDALLDSAASETTDIN
ARYDRYAQAQAWLEDSSLIIPLTVSNGAAPVISRLTPFTGASIQVGDKGSSYVKYVKSQEKVVTKKEYEQSREKWLKERK
ASNEKAQKDLEKHVK

Nucleotide


Download         Length: 1968 bp        

>NTDB_id=23928 STU_RS16130 WP_011225333.1 1276242..1278209(-) (amiA) [Streptococcus thermophilus LMG 18311]
ATGAAACTTAAAAAAATTCTTGGAATTACGGGTGTAGCCCTTCTAAGTGTTGGTATGTTAGCGGCTTGTTCTTCTAAGTC
AAGTACTAGTGGAACGACTTATAGTAATATTTATGGCTCAGATCCAGAAACCTTGGATTATATTACATCAATTATGGGAG
GGACAAAAGCTGTTTTGACTAATGGTGTAGATGGTCTTATGGAAGCGGACAAATATGGTAATCTTGTTCCATCAGTAGCT
GAAGACTGGTCTGTGTCTAAAGATGGTCTGACTTATACCTATAAAATTCGTAAAGGGATCAAATGGTATACTTCTGAAGG
TGAAGAGTACGCTAATGTTACTGCTAAGGACTTTGTAACGGGTCTTAAGCATGCAGCTGATGCCAAATCTGGAGCCCTTT
ATTTGGTACAAGATTCTATTACTGGATTGTCTGACTACCTTTCTGGTGCCAATAAAGATTTCTCAAATGTTGGCGTTAAA
GCAATAGACGATTACACTCTGCAATACACACTAAAAAAACAGGAACCATACTGGAACTCTAAGACAACCTATGGTGTGCT
CTTCCCAGTTAACGAAGACTTTCTTAAGAACAAAGGGAAAGATTTTGGTAAGTCAACAGACCCAACCTCTATCCTATATA
ACGGTCCTTTCCTTCTTAAATCTTTGACAGCTAAATCATCTATCGAGTTGACTAAGAATGAGAATTATTGGGATAAGAAA
AACGTGCATTTTGACTCTATCAAATTCTCATATTCTGATGGATCGGATCTAGAATCAATAGAACGTAGTTTCTCTGACGG
CGCTCTCAGCATTGCACGTGTCTTCCCTACGAGTTCAAACTATGCATCGGTAGAAAAAAAATATAAAGATAATATTTTTT
ACACAGAACCAGGTGCATCTACATCGGTTATTGGTGTAAACATTGACCGCCAAAGCTATAAATTCTCAGCTAAGAAGACA
GATGCTGAAAAGACATCAACTAAGAAGGCTCTTCTAAACAAAGACTTTCGTCAATCAATCAACTTCGCTATTGATCGTAC
AGCCTATCAATCACAAGTAAACGGTAAAGATGGCGCAGCTCTTGCTCTACGTAACCTTTTTGTTCCTTATGATTTTGTCA
GTGCAGGCGATAAGACCTTTGGTGATCTTGTGACAGAAAAAATGAGCTCTTATGGTGATGAGTGGTCTGGTGTTAACTTT
GCAGATGGTCAAGAGGGTCTCTACAATGCTGAAAAAGCTAAGACTGAGTTTGCCAAAGCCAAAGAGGCTCTTCAAGGAGA
AGGGGTACAATTCCCAGTTCACTTAGACCTTCCAGTTGATCAAAGTTCTAAACTTAGTGTTGCTCAAGCTCAATCTTTGA
AACAAACCATTGAAAAATCATTGGGTAGCGAAAATGTTGTTATAGATATTAACCAAATGTCATCTGATGACATGAATAAT
GTGACTAGTAATGCGGCAAATGCTGCAGCAGAAGACTGGGATATTTCTAACGGTGTGGGTTGGGGTCCAGACTATCAAGA
TCCATCAACTTATCTTGATATCTTAAAAACAACATCAAGTGAAAATACCAAGATTTTCAATGGTTATGATGATCCAAACA
ATGCAGCAGCAGCCCAAGTAGGTCTTAAGGATTATGATGCCTTGCTTGATTCAGCAGCTTCAGAAACAACAGACATCAAC
GCTCGTTACGACCGTTATGCTCAAGCCCAAGCTTGGTTGGAAGATAGCTCTCTTATCATTCCATTGACTGTTAGTAACGG
TGCAGCACCAGTTATCTCACGTTTGACACCATTTACGGGTGCTAGCATACAGGTGGGTGATAAGGGCTCATCCTACGTCA
AATATGTGAAATCACAAGAAAAAGTAGTTACTAAGAAAGAATATGAACAAAGTCGTGAAAAATGGCTTAAAGAAAGGAAA
GCATCTAACGAAAAAGCTCAAAAAGATCTTGAGAAACACGTTAAATAA

Domains

Predicted by InterproScan.

Bacterial extracellular solute-binding proteins, family 5 Middle (SBP_bac_5)

(75-520)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M6C6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiA Streptococcus salivarius strain HSISS4

88.72

100

0.889
  amiA3 Streptococcus thermophilus LMG 18311

86.301

100

0.866
  amiA3 Streptococcus thermophilus LMD-9

86.301

100

0.866

Multiple sequence alignment