Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   C8257_RS30930 Genome accession   NZ_CP028162
Coordinates   6426241..6426864 (-) Length   207 a.a.
NCBI ID   WP_003095838.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain MRSN12280     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6421241..6431864
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C8257_RS30910 (C8257_30905) aroB 6421836..6422942 (-) 1107 WP_003095831.1 3-dehydroquinate synthase -
  C8257_RS30915 (C8257_30910) aroK 6422992..6423510 (-) 519 WP_003095833.1 shikimate kinase AroK -
  C8257_RS30920 (C8257_30915) pilQ 6423522..6425666 (-) 2145 WP_003095835.1 type 4a pilus secretin PilQ Machinery gene
  C8257_RS30925 (C8257_30920) comL/pilP 6425720..6426244 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  C8257_RS30930 (C8257_30925) pilO 6426241..6426864 (-) 624 WP_003095838.1 type 4a pilus biogenesis protein PilO Machinery gene
  C8257_RS30935 (C8257_30930) pilN 6426861..6427457 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  C8257_RS30940 (C8257_30935) pilM 6427457..6428521 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  C8257_RS30945 (C8257_30940) - 6428706..6431177 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22805.23 Da        Isoelectric Point: 4.7787

>NTDB_id=238747 C8257_RS30930 WP_003095838.1 6426241..6426864(-) (pilO) [Pseudomonas aeruginosa strain MRSN12280]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=238747 C8257_RS30930 WP_003095838.1 6426241..6426864(-) (pilO) [Pseudomonas aeruginosa strain MRSN12280]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGGTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

44.211

91.787

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.684

91.787

0.401

Multiple sequence alignment