Detailed information NTG0238630

Bioinformatically predicted

Overview

• Name: pknB
• Type: Regulator
• Locus tag: LL14B4_RS10685
• Genome accession: NZ_CP028160
• Coordinates: 2166437..2168320 (-)
• Length: 627 a.a.
• NCBI ID: WP_058211607.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain 14B4
• Function: modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology) Competence regulation

Genomic Context

Location: 2161437..2173320

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL14B4_RS10655 (LL14B4_10645)	-	2161843..2162523 (-)	681	WP_012898438.1	5'-methylthioadenosine/adenosylhomocysteine nucleosidase	-
LL14B4_RS10660 (LL14B4_10650)	macP	2162555..2162833 (-)	279	WP_003130796.1	cell wall synthase accessory phosphoprotein MacP	-
LL14B4_RS10665 (LL14B4_10655)	-	2162826..2163410 (-)	585	WP_033899834.1	NUDIX hydrolase	-
LL14B4_RS10670 (LL14B4_10660)	glmU	2163515..2164891 (-)	1377	WP_003130798.1	bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU	-
LL14B4_RS10675 (LL14B4_10665)	proC	2165119..2165907 (+)	789	WP_033899830.1	pyrroline-5-carboxylate reductase	-
LL14B4_RS10680 (LL14B4_10670)	rpsO	2165986..2166255 (-)	270	WP_015427039.1	30S ribosomal protein S15	-
LL14B4_RS10685 (LL14B4_10675)	pknB	2166437..2168320 (-)	1884	WP_058211607.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator
LL14B4_RS10690 (LL14B4_10680)	-	2168320..2169096 (-)	777	WP_003130802.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
LL14B4_RS10695 (LL14B4_10685)	rsmB	2169177..2170451 (-)	1275	WP_109991297.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
LL14B4_RS10700 (LL14B4_10690)	-	2170650..2171156 (-)	507	WP_012898444.1	Asp23/Gls24 family envelope stress response protein	-
LL14B4_RS10705 (LL14B4_10695)	-	2171177..2171365 (-)	189	WP_033899826.1	DUF2273 domain-containing protein	-
LL14B4_RS10710 (LL14B4_10700)	amaP	2171376..2171936 (-)	561	WP_109991298.1	alkaline shock response membrane anchor protein AmaP	-
LL14B4_RS10715 (LL14B4_10705)	-	2171964..2172206 (-)	243	WP_003131476.1	GlsB/YeaQ/YmgE family stress response membrane protein	-

Sequence

Protein

• Length: 627 a.a.
• Molecular weight: 67948.01 Da
• Isoelectric Point: 4.7401

>NTDB_id=238630 LL14B4_RS10685 WP_058211607.1 2166437..2168320(-) (pknB) [Lactococcus lactis subsp. lactis strain 14B4]
MIQIGKIFADRYRIIKEIGRGGMANVYQGEDTFLGDRLVAIKVLRSNFENDDIAIARFQREAFAMAELSHPNIVGISDVG
EFESQQYIVMEFVDGMTLKQYINQNAPLANDEAIEIITEILSAMDMAHSHGIIHRDLKPQNVLVSSSGTVKVTDFGIAKA
LSETSLTQTNTMFGSVHYLSPEQARGSNATVQSDIYAIGIILFELLTGQIPFDGDSAVAIALKHFQESIPSIINLNPEVP
QALENVVIKATAKDIKNRYTDVEEMMTDVATSTSLDRRGEEKLVFNKDHDETKIMPANLINPYDTKPLIDKKEDNDSQTD
EKAASSEVVNKNKKSKKGLIIGLIILLLVVGGATLAWVVSTPTNVKIPNVTNSTLDQAKSKIKDAKLKVGTVHKQQSSTI
AEGKVIKTDPTSGTTVRSNSSVDIYVSTGNEDIIKMKDFVGEKIDEAMATLLKDYGIDESQVTQTSVPSDSYPAGTIIKQ
SPKKGSSFDTKGSEKITFEVSSGKQVEVPDYKPNGQYMTYSQYQAALKAAGFTNITLDPQATTNQQADGYVYSVYPNVGT
SVDPTQEIVVTYSVYTAPSSSSTTSESTTTSETSSSTTSSTSSSTTSQPSTDNNNSSKESSTTSSSS

Nucleotide

• Length: 1884 bp

>NTDB_id=238630 LL14B4_RS10685 WP_058211607.1 2166437..2168320(-) (pknB) [Lactococcus lactis subsp. lactis strain 14B4]
ATGATTCAAATCGGTAAAATCTTTGCCGATCGTTATCGGATTATCAAAGAAATCGGACGTGGCGGAATGGCGAACGTTTA
TCAAGGTGAAGATACTTTTCTTGGTGACCGCTTAGTTGCAATAAAAGTGCTACGTTCAAATTTTGAAAACGATGATATCG
CGATTGCTCGTTTTCAGCGAGAAGCATTCGCCATGGCAGAACTTTCACATCCAAATATCGTAGGTATTTCTGATGTTGGT
GAATTTGAAAGTCAACAATACATTGTCATGGAATTTGTTGACGGCATGACACTTAAACAATACATCAATCAAAATGCACC
TTTAGCCAATGATGAAGCTATTGAAATTATTACTGAAATTTTATCAGCAATGGACATGGCCCATTCACATGGTATTATTC
ACCGGGATTTAAAACCACAAAATGTTTTAGTTTCCAGTAGTGGAACTGTAAAAGTAACGGATTTTGGAATTGCGAAAGCA
CTCTCTGAAACTTCACTTACTCAAACAAATACAATGTTTGGTTCGGTTCATTATTTATCACCAGAGCAAGCGCGTGGTTC
AAATGCAACTGTTCAATCAGATATTTATGCGATTGGAATTATTTTATTTGAACTTTTAACAGGTCAAATTCCATTTGATG
GAGATTCTGCAGTTGCTATCGCTCTAAAACATTTCCAAGAGAGTATTCCAAGCATCATCAATCTCAATCCAGAAGTTCCG
CAAGCTTTGGAAAATGTTGTCATTAAAGCTACAGCGAAAGATATTAAAAATCGCTATACTGATGTAGAAGAAATGATGAC
TGATGTGGCAACTTCAACGAGTCTTGACCGTCGTGGGGAAGAAAAATTAGTTTTCAATAAGGACCATGATGAAACAAAAA
TTATGCCTGCCAATCTAATTAATCCTTATGACACCAAGCCTCTGATTGATAAAAAAGAAGATAATGATTCACAAACAGAT
GAAAAAGCTGCAAGCTCAGAAGTAGTAAATAAAAACAAAAAATCTAAAAAAGGCTTGATTATTGGTCTTATTATCCTTCT
TTTAGTTGTTGGCGGTGCTACTTTGGCTTGGGTTGTCTCAACACCAACTAATGTTAAGATACCTAATGTGACAAACTCGA
CGCTTGACCAAGCAAAAAGCAAAATTAAAGATGCAAAACTAAAAGTGGGAACAGTTCATAAACAACAAAGTTCAACGATT
GCTGAAGGAAAAGTTATCAAGACTGATCCAACTAGTGGAACAACCGTGAGATCTAATAGCTCCGTTGATATTTATGTTTC
TACGGGAAATGAAGATATCATCAAAATGAAGGACTTTGTCGGTGAAAAGATTGATGAGGCGATGGCAACATTACTTAAGG
ATTATGGTATTGATGAAAGCCAAGTCACTCAAACTTCCGTTCCTAGTGATTCTTACCCAGCAGGTACAATCATTAAACAA
AGCCCTAAAAAAGGTTCTAGTTTTGATACCAAAGGGAGTGAAAAAATTACCTTTGAAGTTTCAAGTGGAAAACAAGTTGA
AGTTCCGGATTATAAACCGAACGGTCAATATATGACTTATTCACAGTATCAAGCTGCCTTAAAAGCTGCAGGATTCACTA
ATATTACACTAGACCCTCAAGCAACAACGAATCAACAAGCTGATGGTTATGTTTACTCAGTGTATCCAAATGTAGGGACA
TCTGTTGATCCAACTCAAGAAATTGTAGTCACTTATAGCGTCTATACTGCTCCAAGTTCAAGTTCAACAACTTCTGAATC
AACTACGACGTCTGAAACTTCATCAAGTACAACTTCAAGTACTTCAAGCTCTACAACAAGTCAGCCGTCAACAGATAACA
ACAATAGCAGCAAAGAATCTTCAACAACGTCATCAAGTAGTTGA

Domains

Predicted by InterProScan.

• Protein kinase domain (Pkinase) (12-265)

• PASTA domain (PASTA) (365-428)

• PASTA domain (PASTA) (506-573)

• PASTA domain (PASTA) (434-501)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pknB	Streptococcus mutans UA159	49.081	86.762	0.426
stkP/pknB	Streptococcus salivarius strain HSISS4	51.252	82.775	0.424
stkP	Streptococcus pneumoniae TIGR4	48.024	80.702	0.388
stkP	Streptococcus pneumoniae D39	47.826	80.702	0.386
stkP	Streptococcus pneumoniae R6	47.826	80.702	0.386
stkP	Streptococcus pneumoniae Rx1	47.628	80.702	0.384