Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CEQ48_RS05845 Genome accession   NZ_CP022353
Coordinates   81233..82249 (+) Length   338 a.a.
NCBI ID   WP_089070616.1    Uniprot ID   A0AAU8WQG5
Organism   Vibrio tarriae strain 2521-89     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 76233..87249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEQ48_RS05830 (CEQ48_05830) - 76773..77504 (-) 732 WP_198301215.1 glutathione peroxidase -
  CEQ48_RS05835 (CEQ48_05835) oxyR 77644..78537 (+) 894 WP_085603459.1 DNA-binding transcriptional regulator OxyR -
  CEQ48_RS05840 (CEQ48_05840) - 78613..81090 (-) 2478 WP_198301216.1 penicillin-binding protein 1A -
  CEQ48_RS05845 (CEQ48_05845) pilM 81233..82249 (+) 1017 WP_089070616.1 type IV pilus assembly protein PilM Machinery gene
  CEQ48_RS05850 (CEQ48_05850) pilN 82239..82823 (+) 585 WP_181710719.1 PilN domain-containing protein Machinery gene
  CEQ48_RS05855 (CEQ48_05855) pilO 82816..83406 (+) 591 WP_181710720.1 type 4a pilus biogenesis protein PilO Machinery gene
  CEQ48_RS05860 (CEQ48_05860) pilP 83396..83914 (+) 519 WP_089070619.1 pilus assembly protein PilP Machinery gene
  CEQ48_RS05865 (CEQ48_05865) pilQ 83940..85655 (+) 1716 WP_181710721.1 type IV pilus secretin PilQ Machinery gene
  CEQ48_RS05870 (CEQ48_05870) aroK 85842..86366 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37791.47 Da        Isoelectric Point: 7.0725

>NTDB_id=238615 CEQ48_RS05845 WP_089070616.1 81233..82249(+) (pilM) [Vibrio tarriae strain 2521-89]
MGKTLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEPREQEFAVYQAFSHQSPFPVEELSLDFVKATEKSLARSSTTTFQVYATKKEVVDSRLQAIKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLIDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEESGVISLNSPLQQDPTQR
FIHEFVEKVARQIQLFTSVHGAQSLGGLWLSGGGATLPGLEEALYQRLSLHCEILNPFSLFKNNVAKRKRQVIDGQRFST
AAGLALRGLAWLESDHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=238615 CEQ48_RS05845 WP_089070616.1 81233..82249(+) (pilM) [Vibrio tarriae strain 2521-89]
ATGGGTAAAACATTAGTTACGGGTATTGATATCGGCCACCACAGTATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGGTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCCGCGCGAGCAGGAATTTGCTGTCTATCAAGCCTTCTCCCA
TCAATCTCCGTTTCCCGTTGAGGAGCTTAGCCTCGATTTTGTCAAAGCGACGGAAAAAAGCCTCGCTCGCAGTAGCACCA
CGACATTCCAAGTGTATGCCACCAAAAAAGAGGTGGTCGACAGCCGTTTACAAGCTATTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTGCATAGTTTGCTTCATCTATGGCAACTCGCTAGCCGAGCTTATCGACGTCCAGACTGGAT
GCTCATTGATATTGGCTACACCCAAAGTTCATTGTGCCTTGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAC
TAGGAACTCGTCAATTGGAATCGGAAGAAAGTGGCGTGATCTCATTGAATTCGCCACTGCAACAAGACCCAACACAACGT
TTCATTCACGAATTTGTCGAAAAAGTTGCGCGACAAATTCAGCTCTTTACTTCGGTGCATGGTGCTCAAAGCCTTGGTGG
TTTATGGCTATCGGGTGGTGGTGCCACACTTCCTGGCCTAGAAGAGGCGCTCTACCAACGCTTATCACTACATTGTGAAA
TTTTGAACCCGTTTTCGCTGTTCAAAAACAATGTGGCAAAGCGTAAACGCCAAGTGATTGATGGCCAGCGTTTTAGTACT
GCAGCAGGTTTGGCGCTGCGAGGCTTAGCTTGGTTGGAGAGTGACCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

92.012

100

0.92
  pilM Vibrio campbellii strain DS40M4

48.406

100

0.494

Multiple sequence alignment