Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   CFI09_RS02370 Genome accession   NZ_CP022279
Coordinates   462450..463574 (+) Length   374 a.a.
NCBI ID   WP_000228508.1    Uniprot ID   A0A0D8VMA7
Organism   Escherichia coli strain STEC299     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 457450..468574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFI09_RS02345 (CFI09_02355) mscL 457567..457980 (-) 414 WP_000022450.1 large-conductance mechanosensitive channel protein MscL -
  CFI09_RS02350 (CFI09_02360) trkA 458110..459486 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  CFI09_RS02355 (CFI09_02365) rsmB 459508..460797 (-) 1290 WP_000744767.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  CFI09_RS02360 (CFI09_02370) fmt 460849..461796 (-) 948 WP_101973432.1 methionyl-tRNA formyltransferase -
  CFI09_RS02365 (CFI09_02375) def 461811..462320 (-) 510 WP_000114984.1 peptide deformylase -
  CFI09_RS02370 (CFI09_02380) dprA 462450..463574 (+) 1125 WP_000228508.1 DNA-protecting protein DprA Machinery gene
  CFI09_RS02375 (CFI09_02385) smg 463546..464019 (+) 474 WP_000460672.1 DUF494 family protein Smg -
  CFI09_RS02380 (CFI09_02390) yrdD 464048..464590 (+) 543 WP_001312137.1 type I DNA topoisomerase -
  CFI09_RS02385 (CFI09_02395) tsaC 464595..465167 (+) 573 WP_001296460.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  CFI09_RS02390 (CFI09_02400) aroE 465172..465990 (+) 819 WP_000451247.1 shikimate dehydrogenase -
  CFI09_RS02395 (CFI09_02405) yrdB 465987..466244 (+) 258 WP_001070567.1 DUF1488 domain-containing protein -
  CFI09_RS02400 (CFI09_02410) yrdA 466220..466774 (-) 555 WP_001286216.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40892.82 Da        Isoelectric Point: 6.0395

>NTDB_id=237872 CFI09_RS02370 WP_000228508.1 462450..463574(+) (dprA) [Escherichia coli strain STEC299]
MVDTEIWLRLISISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=237872 CFI09_RS02370 WP_000228508.1 462450..463574(+) (dprA) [Escherichia coli strain STEC299]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATTAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCTCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT
TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (DprA_WH)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D8VMA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Haemophilus influenzae Rd KW20

43.917

90.107

0.396
  dprA Legionella pneumophila strain ERS1305867

44.242

88.235

0.39

Multiple sequence alignment