Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   MPAO1_RS21650 Genome accession   NZ_CP027857
Coordinates   4690275..4690946 (-) Length   223 a.a.
NCBI ID   WP_003085524.1    Uniprot ID   A0A0H2Z6L5
Organism   Pseudomonas aeruginosa strain MPAO1     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4685275..4695946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPAO1_RS21625 (MPAO1_22200) - 4686048..4686500 (-) 453 WP_003114181.1 protein YgfX -
  MPAO1_RS21630 (MPAO1_22205) - 4686484..4686738 (-) 255 WP_003085532.1 succinate dehydrogenase assembly factor 2 -
  MPAO1_RS21635 (MPAO1_22210) - 4687011..4687955 (+) 945 WP_003114180.1 folate-binding protein YgfZ -
  MPAO1_RS21640 (MPAO1_22215) - 4688056..4688892 (+) 837 WP_003114179.1 HDOD domain-containing protein -
  MPAO1_RS21645 (MPAO1_22220) - 4688900..4690282 (-) 1383 WP_003114178.1 sensor histidine kinase -
  MPAO1_RS21650 (MPAO1_22225) ciaR 4690275..4690946 (-) 672 WP_003085524.1 response regulator Regulator
  MPAO1_RS21655 (MPAO1_22230) - 4691157..4692440 (+) 1284 WP_003114177.1 OprD family porin -
  MPAO1_RS21660 (MPAO1_22235) - 4692470..4693453 (+) 984 WP_003085519.1 tripartite tricarboxylate transporter substrate binding protein -
  MPAO1_RS21665 (MPAO1_22240) - 4693502..4693972 (+) 471 WP_003114175.1 tripartite tricarboxylate transporter TctB family protein -
  MPAO1_RS21670 (MPAO1_22245) - 4693983..4695500 (+) 1518 WP_003114173.1 tripartite tricarboxylate transporter permease -

Sequence

Protein


Download         Length: 223 a.a.        Molecular weight: 24431.24 Da        Isoelectric Point: 5.1881

>NTDB_id=237528 MPAO1_RS21650 WP_003085524.1 4690275..4690946(-) (ciaR) [Pseudomonas aeruginosa strain MPAO1]
MRILLVEDHPQLAESVVQALKGAGWTVDLLQDGVAADLALASEEYALAILDVGLPRMDGFEVLARLRGRGKTLPVLMLTA
RGEVKDRVHGLNLGADDYLAKPFELSELEARVKALLRRSVLGGEQLQRCGALVYDLGTRRFSLDEQPLTLTSREQAVLEA
MIARPGRVMSKEQLAAQVFGLDEEASADAIEIYVHRLRKKLEGGAVRIVTFRGLGYLLEAQGD

Nucleotide


Download         Length: 672 bp        

>NTDB_id=237528 MPAO1_RS21650 WP_003085524.1 4690275..4690946(-) (ciaR) [Pseudomonas aeruginosa strain MPAO1]
ATGCGCATCCTTCTGGTGGAAGATCATCCGCAGCTCGCCGAAAGCGTGGTCCAGGCGCTCAAGGGCGCCGGCTGGACGGT
AGACCTGCTGCAGGACGGGGTGGCCGCCGACCTGGCCCTGGCCAGCGAGGAGTACGCCCTGGCGATCCTCGACGTCGGCC
TGCCGCGGATGGACGGCTTCGAGGTGCTGGCGCGCCTGCGCGGGCGCGGCAAGACCCTGCCGGTGCTGATGCTGACTGCG
CGCGGCGAGGTGAAGGACCGGGTGCACGGCCTCAATCTCGGCGCCGACGACTACCTGGCAAAGCCTTTCGAACTGTCCGA
GCTGGAGGCGCGGGTCAAGGCCCTGCTGCGCCGCAGCGTGCTCGGTGGCGAACAGTTGCAGCGCTGCGGCGCCCTGGTCT
ACGACCTCGGCACCCGGCGCTTCAGCCTCGACGAGCAGCCGCTGACCCTGACCTCGCGGGAACAGGCGGTGCTGGAAGCG
ATGATCGCCCGTCCGGGGCGGGTGATGAGCAAGGAGCAACTGGCCGCCCAGGTCTTCGGCCTGGACGAGGAGGCCAGCGC
CGACGCCATCGAGATCTACGTCCACCGCCTGCGCAAGAAGCTGGAAGGCGGCGCCGTGCGCATCGTCACCTTCCGCGGCC
TCGGCTACCTGCTGGAGGCGCAGGGTGACTGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(146-218)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z6L5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.668

100

0.377
  ciaR Streptococcus pneumoniae D39

37.668

100

0.377
  ciaR Streptococcus pneumoniae R6

37.668

100

0.377
  ciaR Streptococcus pneumoniae TIGR4

37.668

100

0.377
  covR Lactococcus lactis subsp. lactis strain DGCC12653

37.668

100

0.377

Multiple sequence alignment