Detailed information NTG0023745

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: M6_RS00575
• Genome accession: NC_006086
• Coordinates: 95268..96266 (+)
• Length: 332 a.a.
• NCBI ID: WP_002986679.1
• Uniprot ID: A2RC05
• Organism: Streptococcus pyogenes MGAS10394
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 90268..101266

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M6_RS00560 (M6_Spy0081)	-	91152..92492 (+)	1341	WP_011184068.1	hypothetical protein	-
M6_RS00565 (M6_Spy0082)	purB	92708..94000 (+)	1293	WP_011184069.1	adenylosuccinate lyase	-
M6_RS00570 (M6_Spy0083)	comR	94131..95042 (+)	912	WP_002986681.1	transcriptional regulator Rgg4/ComR	Regulator
M6_RS10415	comS	95126..95221 (+)	96	WP_198462994.1	quorum-sensing system DWW-type pheromone	Regulator
M6_RS00575 (M6_Spy0084)	ruvB	95268..96266 (+)	999	WP_002986679.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
M6_RS00580 (M6_Spy0085)	-	96404..96841 (+)	438	WP_002994668.1	low molecular weight protein-tyrosine-phosphatase	-
M6_RS00585 (M6_Spy0086)	-	96864..97265 (+)	402	WP_002986671.1	MORN repeat-containing protein	-
M6_RS00590 (M6_Spy0087)	-	97262..99037 (+)	1776	WP_011184071.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37565.11 Da
• Isoelectric Point: 5.3005

>NTDB_id=23745 M6_RS00575 WP_002986679.1 95268..96266(+) (ruvB) [Streptococcus pyogenes MGAS10394]
MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEAAKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAVEKAGDLVAILNELEPGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPRIANRLLKRVRDYAQIIGDGIIT
AQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGGPVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQK
AYRHLGYPYQNT

Nucleotide

• Length: 999 bp

>NTDB_id=23745 M6_RS00575 WP_002986679.1 95268..96266(+) (ruvB) [Streptococcus pyogenes MGAS10394]
ATGGCTAGAATTTTAGACAATAATGTAATGGGAAATGAGGAGTTTTCGGATCGGACTCTTCGCCCTCAATATCTACACGA
ATATATTGGACAAGATAAAGTTAAGGAACAATTCGCTATTTTTATAGAAGCAGCTAAAAGACGTGACGAATCTTTAGACC
ATGTTTTATTATTTGGACCTCCAGGACTGGGAAAAACAACAATGGCATTTGTGATTGCCAATGAATTGGGAGTTAATTTA
AAGCAAACTTCAGGTCCTGCCGTTGAGAAAGCTGGAGATTTGGTAGCTATTTTAAATGAATTAGAACCAGGGGATATTTT
GTTTATTGATGAAATTCACCGCATGCCAATGTCTGTCGAGGAAGTACTATATAGTGCTATGGAAGACTTCTATATTGATA
TTATGATTGGCGCTGGGGATACTAGTCGTAGTATTCATTTGGATCTGCCTCCATTTACATTGATTGGTGCTACTACACGT
GCAGGCATGTTATCTAATCCCCTCAGAGCTCGTTTTGGTATCACTGGTCACATGGAATACTATCAAGAAAAGGATTTGAC
AGAGATTGTGGAGCGTACAGCTACTATTTTTGAAATAAAAATAGACCATGAAGCTGCACGGAAATTAGCTTGTCGCAGTA
GAGGAACGCCCCGCATTGCTAATCGATTACTAAAACGAGTGCGTGACTATGCTCAAATTATTGGTGATGGCATCATAACG
GCTCAAATAACTGATAGAGCTTTGACTATGTTAGATGTTGACCGCGAAGGTCTCGATTACATTGATCAGAAAATTTTACG
AACGATGATTGAGATGTATCAGGGAGGACCAGTTGGTTTGGGGACATTATCTGTGAATATTGCCGAGGAGCGTAATACAG
TTGAAGAGATGTATGAACCCTACTTAATTCAAAAAGGCTTTCTCATGCGAACACGAACAGGTCGTGTAGCGACGCAAAAG
GCTTATCGTCATCTTGGCTATCCCTATCAAAACACTTAA

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: A2RC05

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae D39	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae TIGR4	84.545	99.398	0.84
ruvB	Bacillus subtilis subsp. subtilis str. 168	58.824	97.289	0.572
ruvB	Helicobacter pylori 26695	48.936	99.096	0.485
ruvB	Synechocystis sp. PCC 6803	50.16	94.277	0.473