Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CEK71_RS16265 Genome accession   NZ_CP022129
Coordinates   3590871..3591932 (-) Length   353 a.a.
NCBI ID   WP_088620362.1    Uniprot ID   A0A1Z4C1W7
Organism   Methylovulum psychrotolerans strain HV10_M2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3585871..3596932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEK71_RS16240 (CEK71_16240) aroK 3585987..3586511 (-) 525 WP_088620357.1 shikimate kinase AroK -
  CEK71_RS16245 (CEK71_16245) pilQ 3586659..3589022 (-) 2364 WP_088620358.1 type IV pilus secretin PilQ -
  CEK71_RS16250 (CEK71_16250) - 3589038..3589598 (-) 561 WP_198328271.1 pilus assembly protein PilP -
  CEK71_RS16255 (CEK71_16255) - 3589595..3590269 (-) 675 WP_088620360.1 type 4a pilus biogenesis protein PilO -
  CEK71_RS16260 (CEK71_16260) - 3590266..3590871 (-) 606 WP_088620361.1 PilN domain-containing protein -
  CEK71_RS16265 (CEK71_16265) pilM 3590871..3591932 (-) 1062 WP_088620362.1 pilus assembly protein PilM Machinery gene
  CEK71_RS16270 (CEK71_16270) - 3592282..3595422 (-) 3141 WP_088620363.1 DUF748 domain-containing protein -
  CEK71_RS16275 (CEK71_16275) - 3595527..3596822 (-) 1296 WP_088620364.1 ammonium transporter -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38733.32 Da        Isoelectric Point: 4.8849

>NTDB_id=237078 CEK71_RS16265 WP_088620362.1 3590871..3591932(-) (pilM) [Methylovulum psychrotolerans strain HV10_M2]
MKWFSHKQNTVLSIDISTAAVKMLELSRQGPRYRVESYAVAPLPKDAVIDKNVANVNMIADTIKIALKQSGTKLKQATVA
VAGSSVMTKIIPMDASLSEDEMEEQIMIEADQYVPYSLDEVNFDFEVQGFNDSNPGMVDVLLAASRRENVDNRVEALAKA
GLKAQIVDVEAFAMENAFTLLVEQFADSIEGQTVAIADIGATITTLNVLHSSRSVYTREQGFGGKQLTEEIQRRYGLSYE
EAGLAKRHGGLPDNYTTDVLDPFKRAMLQQIQRSLQFFVSSSANRSIDRIILAGGCASIQGVDQLIEKNLGIPVYIANPF
LNMKLSNKVKPQILSNDAPAMMIACGLALRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=237078 CEK71_RS16265 WP_088620362.1 3590871..3591932(-) (pilM) [Methylovulum psychrotolerans strain HV10_M2]
ATGAAATGGTTTAGTCATAAACAGAATACTGTTTTGAGTATTGACATCAGTACGGCAGCGGTAAAAATGCTGGAATTAAG
CCGTCAAGGCCCACGTTACCGGGTCGAAAGCTATGCAGTTGCCCCTTTGCCTAAAGATGCTGTCATTGATAAAAACGTCG
CCAATGTCAACATGATTGCCGACACCATCAAAATTGCCTTAAAACAATCTGGCACAAAATTAAAGCAAGCTACCGTAGCC
GTAGCAGGCTCTTCAGTCATGACCAAAATTATCCCCATGGACGCTTCCCTCAGTGAAGACGAAATGGAAGAGCAAATCAT
GATTGAAGCTGACCAATATGTGCCCTATTCCCTTGATGAGGTCAATTTTGATTTTGAAGTACAGGGGTTCAATGACAGCA
ACCCCGGTATGGTTGATGTCCTGCTGGCGGCTTCCAGACGGGAAAATGTCGACAACCGTGTCGAGGCATTGGCAAAGGCA
GGCTTAAAAGCCCAAATCGTCGATGTTGAAGCTTTTGCTATGGAAAATGCCTTTACTTTATTGGTCGAACAGTTTGCCGA
TTCCATAGAAGGCCAAACTGTCGCTATTGCCGATATTGGCGCTACCATTACCACCCTCAACGTCCTGCATAGCAGCCGCA
GTGTCTACACCCGCGAGCAAGGCTTTGGCGGCAAGCAACTGACCGAAGAAATCCAACGCCGTTACGGGCTGTCTTACGAA
GAGGCGGGGCTGGCAAAACGTCATGGCGGCTTACCGGACAATTACACCACTGATGTCTTAGACCCCTTTAAACGCGCTAT
GCTCCAGCAAATCCAACGGTCTTTACAGTTTTTTGTCTCGTCAAGCGCCAACCGTAGTATTGACCGGATTATCTTGGCAG
GCGGCTGCGCTTCAATACAAGGTGTCGACCAGCTTATTGAAAAAAACTTGGGGATACCTGTTTACATCGCCAACCCATTC
CTGAACATGAAGCTATCCAACAAAGTAAAACCCCAGATTTTAAGCAATGATGCACCAGCAATGATGATTGCCTGTGGCTT
GGCATTAAGGAGTTTTGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z4C1W7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

49.014

100

0.493
  comM Acinetobacter nosocomialis M2

49.014

100

0.493
  comM Acinetobacter baylyi ADP1

47.887

100

0.482
  pilM Legionella pneumophila strain ERS1305867

41.36

100

0.414

Multiple sequence alignment