Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CEP47_RS06755 Genome accession   NZ_CP022013
Coordinates   1437270..1437737 (-) Length   155 a.a.
NCBI ID   WP_261920343.1    Uniprot ID   A0A8E3MDR2
Organism   Mergibacter septicus strain 27517-4-I1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1432270..1442737
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEP47_RS06725 (CEP47_06660) - 1432670..1432942 (-) 273 WP_261920967.1 GNAT family N-acetyltransferase -
  CEP47_RS06730 (CEP47_06665) yacG 1432982..1433188 (-) 207 WP_261920348.1 DNA gyrase inhibitor YacG -
  CEP47_RS06735 (CEP47_06670) coaE 1433181..1433819 (-) 639 WP_261920347.1 dephospho-CoA kinase -
  CEP47_RS06740 (CEP47_06675) - 1433858..1434547 (-) 690 WP_261920346.1 prepilin peptidase -
  CEP47_RS06745 (CEP47_06680) - 1434589..1435815 (-) 1227 WP_261920345.1 type II secretion system F family protein -
  CEP47_RS06750 (CEP47_06685) pilB 1435830..1437248 (-) 1419 WP_261920344.1 GspE/PulE family protein Machinery gene
  CEP47_RS06755 (CEP47_06690) pilA 1437270..1437737 (-) 468 WP_261920343.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CEP47_RS06760 (CEP47_06695) ampD 1437864..1438463 (+) 600 WP_373463079.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CEP47_RS06765 (CEP47_06700) - 1438626..1439693 (+) 1068 WP_261920342.1 LysM-like peptidoglycan-binding domain-containing protein -
  CEP47_RS06770 (CEP47_06705) cgtA 1439755..1440915 (-) 1161 WP_261920341.1 Obg family GTPase CgtA -
  CEP47_RS06775 (CEP47_06710) - 1440959..1441891 (-) 933 WP_261920340.1 DMT family transporter -
  CEP47_RS06780 (CEP47_06715) rpmA 1442063..1442320 (-) 258 WP_261920339.1 50S ribosomal protein L27 -
  CEP47_RS06785 (CEP47_06720) rplU 1442341..1442652 (-) 312 WP_261920338.1 50S ribosomal protein L21 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16212.65 Da        Isoelectric Point: 7.5827

>NTDB_id=235890 CEP47_RS06755 WP_261920343.1 1437270..1437737(-) (pilA) [Mergibacter septicus strain 27517-4-I1]
MKRSTLTQAALSRVGLSQGFTLIELMIVIAIIAVLATVAIPSYQNYTKKAAMSELIQAASPYKAEVELCIYNSGSKTNCS
AGQNGIQAAVTESEKVKYLKTVGVNAGVISVTGKGTLENIGYTLTPNGGENGEVIDWAVNCTGDKGIFPVGFCAN

Nucleotide


Download         Length: 468 bp        

>NTDB_id=235890 CEP47_RS06755 WP_261920343.1 1437270..1437737(-) (pilA) [Mergibacter septicus strain 27517-4-I1]
ATGAAAAGATCAACCTTAACTCAAGCAGCTTTATCTCGAGTTGGATTATCTCAAGGCTTTACTTTAATTGAATTGATGAT
TGTCATCGCAATCATTGCTGTATTAGCCACGGTGGCTATTCCTTCCTATCAAAACTATACCAAGAAAGCTGCAATGTCAG
AATTGATTCAAGCTGCTTCGCCGTATAAGGCTGAAGTTGAGTTATGTATTTATAATTCAGGTTCTAAGACAAATTGCTCA
GCAGGGCAAAATGGTATTCAAGCGGCGGTTACTGAGAGTGAGAAAGTAAAATATTTGAAAACGGTCGGAGTAAATGCAGG
TGTTATTTCAGTAACAGGTAAAGGAACATTGGAGAATATTGGATATACACTGACGCCAAATGGTGGCGAAAATGGAGAGG
TCATTGATTGGGCTGTAAACTGTACGGGGGATAAAGGAATATTTCCAGTAGGTTTTTGTGCTAATTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(17-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

55.396

89.677

0.497
  pilA Glaesserella parasuis strain SC1401

53.901

90.968

0.49
  pilA Haemophilus influenzae Rd KW20

53.957

89.677

0.484
  pilA Vibrio campbellii strain DS40M4

38.356

94.194

0.361

Multiple sequence alignment