Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   C6W75_RS01155 Genome accession   NZ_CP027462
Coordinates   239551..240789 (+) Length   412 a.a.
NCBI ID   WP_000815987.1    Uniprot ID   Q3YWN2
Organism   Escherichia coli isolate 07-4299     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 234551..245789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6W75_RS01130 mrcA 234831..237383 (-) 2553 WP_000673804.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  C6W75_RS01135 hofM 237503..238282 (+) 780 WP_001315880.1 DNA utilization protein HofM -
  C6W75_RS01140 hofN 238282..238821 (+) 540 WP_001069315.1 DNA utilization protein HofN -
  C6W75_RS01145 hofO 238805..239245 (+) 441 WP_001055763.1 DNA utilization protein HofO -
  C6W75_RS01150 hofP 239235..239639 (+) 405 WP_001264141.1 DNA utilization protein HofP -
  C6W75_RS01155 comE 239551..240789 (+) 1239 WP_000815987.1 DNA uptake porin HofQ Machinery gene
  C6W75_RS01160 aroK 241190..241711 (+) 522 WP_000818618.1 shikimate kinase AroK -
  C6W75_RS01165 aroB 241768..242856 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  C6W75_RS01170 damX 242948..244234 (+) 1287 WP_000343202.1 cell division protein DamX -
  C6W75_RS01175 dam 244341..245177 (+) 837 WP_000742143.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44716.24 Da        Isoelectric Point: 6.3183

>NTDB_id=234700 C6W75_RS01155 WP_000815987.1 239551..240789(+) (comE) [Escherichia coli isolate 07-4299]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=234700 C6W75_RS01155 WP_000815987.1 239551..240789(+) (comE) [Escherichia coli isolate 07-4299]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGTCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGCTTGCTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YWN2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.005

100

0.388
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.53

100

0.376
  pilQ Vibrio cholerae strain A1552

37.53

100

0.376
  comE Glaesserella parasuis strain SC1401

35.952

100

0.367

Multiple sequence alignment